FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003034492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003034492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90
Sequences flagged as poor quality0
Sequence length211-220
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT55.555555555555555No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT55.555555555555555No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC44.444444444444445No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA33.3333333333333335No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT33.3333333333333335No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC33.3333333333333335No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT33.3333333333333335No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT33.3333333333333335No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC33.3333333333333335No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC33.3333333333333335No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA33.3333333333333335No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT33.3333333333333335No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA22.2222222222222223No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG22.2222222222222223No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC22.2222222222222223No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG22.2222222222222223No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG22.2222222222222223No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG22.2222222222222223No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG22.2222222222222223No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT22.2222222222222223No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTAGTCGAGTGCTATAGGCGCTTGT11.1111111111111112No Hit
GAGGGTGAACTCACTGGACCGGGGATGCTTGCATGTGTAATCTTACTAAG11.1111111111111112No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC11.1111111111111112No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA11.1111111111111112No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTTATTAGGAGGG11.1111111111111112No Hit
ACTCCCTTTTAGCCAAGATTGTGCCTATTGCCATACTTGTCTTTGCCGCC11.1111111111111112No Hit
ATTAAGTGGTTGGCTCAGGAGTTTTATAGTTCTTGGACAGTGAGAGTGAG11.1111111111111112No Hit
ACCTACGCGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT11.1111111111111112No Hit
TAGGGAGTGGAAGGGAAATAACATGGCTAGGCCGGGGGTCATTAGGAGGG11.1111111111111112No Hit
TCGCCGATACGGTTGTATAGGATTGCTAGAATGGCTGCTGTGTTGGCATC11.1111111111111112No Hit
CGCAACCTCACCACCACCTTCTTCGACCCCGCCGGAGGCGGAGACCCCAT11.1111111111111112No Hit
CATTTTCGGGTTATGGGCCCGATAGCTGATTTAGCTGACCTTACTTTAGG11.1111111111111112No Hit
AGTGGGTGTTAGGGGGTATGAGAGAAGAGATAAGGAACAGCTATAGTATT11.1111111111111112No Hit
CACCCTCTAAATCAGCGCGAGCAAAAGGGACAAGCATCAATGACGCAGCA11.1111111111111112No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG11.1111111111111112No Hit
CGAGCAGTAGCCCAAACAATCTCATACGAAGTTACCCTAGCCATCATTCT11.1111111111111112No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG11.1111111111111112No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG11.1111111111111112No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT11.1111111111111112No Hit
TATAGGCTTCAGGGTGACTGAAAAATGAGAATAGGAGTTGGTATAGAATG11.1111111111111112No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG11.1111111111111112No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT11.1111111111111112No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGAACATTCTATTTCCCCCTCTAT11.1111111111111112No Hit
TCTCCTTTCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA11.1111111111111112No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCGTCGCCTT11.1111111111111112No Hit
TAATTCTTCTCACCCTAACAGGTCAATCTCGCTTCCCCACCCTTACTAAC11.1111111111111112No Hit
AAGTATACTTGAGGAGGGTGACGGGAGGTGTGTACGCGCTTCAGGGCCCT11.1111111111111112No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA11.1111111111111112No Hit
TTGAGGGATAGTACGAGAATGGGGGGTAGGGGTATGAACATGAGGGTGTT11.1111111111111112No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC11.1111111111111112No Hit
GGGCTATTTAGGCTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACA11.1111111111111112No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG11.1111111111111112No Hit
AATCCTATCCTTACTCGCCTCATTACTTTTCCTTCTATCTCCCCCTCTAT11.1111111111111112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)