FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003034834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003034834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805757
Sequences flagged as poor quality0
Sequence length223-233
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGA600517.452743196770243No Hit
GACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT446165.537153260846632No Hit
CAATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCAC376144.668156776794989No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTA348984.331082447934055No Hit
CAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTA297073.686843552088285No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA279243.4655609569634516No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACT275533.419517298639664No Hit
TCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT263753.2733193754444576No Hit
CTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG261573.246264072170642No Hit
GATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT245793.050423390674856No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTAGTATTGGTTATGGTTC243533.022375232235029No Hit
AGCCTTCTCCACTTCAAGCCAACTAGGACTCATAATAGTTACAATCGGCA217652.7011865860302797No Hit
CTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTA209512.600163572888601No Hit
TGCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGT207092.57012970411675No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA195332.4241799947130462No Hit
TATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTT190752.367339036458883No Hit
CCTGCCCCTACTCCTCCTAGACCAAACCTGACTAGAAAAGCTATTACCTA186932.3199302022818293No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC183272.274507078436799No Hit
CATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACT180972.245962492413966No Hit
TAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTAT165832.058064652246273No Hit
CTAGCCCCTACCCCCCAACTAGGAGGGCACTGGCCCCCAACAGGCATCAC162112.011896887026734No Hit
GAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTACTAAGAAGA160971.9977487009110686No Hit
GGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCACGGTTAGGGT160791.9955147767875425No Hit
GGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAG158731.9699487562627442No Hit
ACTGATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATG136161.6898394925517246No Hit
CGCCCACGGACTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACT123111.527879993596084No Hit
TACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCAT121361.5061612868395806No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCA117361.4565185285390012No Hit
CAGGTGGTCAAGTATTTATGGTACTGTACAATATTCATGGTGGCTGGCAG112361.3944650806632768No Hit
TAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATA104061.2914563571895745No Hit
CCCCCTCTACCCCCTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACC103581.285499226193505No Hit
AGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGG96141.1931636957544272No Hit
AACCCCCCTCCTAATACTAACTACCTGACTCTTACCCCTCACAATCATGG92021.1420316547048304No Hit
GTCATTGGTGTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATT92011.141907547809079No Hit
ACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAGAAATTTAGGT74400.9233553043907778No Hit
ATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTA73990.9182669216649685No Hit
CATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAACGAA72090.8946866114721933No Hit
TTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGC70410.8738366529859499No Hit
AAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGC62950.7812529087553692No Hit
GGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAAT59760.7416628090106571No Hit
GGTGATAAGCTCTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTT57420.7126217954048181No Hit
TTGGCTTGCCATGATTGTGAGGGGTAAGAGTCAGGTAGTTAGTATTAGGA53200.6602486853977068No Hit
TACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCTCC21610.2681950017188805No Hit
TTTAAGTGCTGTGGCCAGAAGTGGGGGGAGGGGGGGGTTTGGCGGAAATT18990.23567899503200096No Hit
TCCCCCAGGCTCCTGGCAGCTCTAGGCAATATCAAGCCTCCTAGAGAGGA14710.18256124365038096No Hit
AGGGGCAGGTTTTGGCTCGTAAGAAGGCCTAGATAGGGGATTGTGCGGTG12410.15401665762754777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)