FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003034930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003034930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425755
Sequences flagged as poor quality0
Sequence length223-233
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTA209634.923723737830443No Hit
CCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT185594.359079752439783No Hit
CACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCA167663.93794553205482No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA164773.8700661178377236No Hit
CTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG159913.7559159610574158No Hit
GACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT154733.6342497445714086No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACT147743.4700708153750397No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTA146603.4432948526734863No Hit
GATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT138723.258211882420641No Hit
TAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGA129923.0515202405139106No Hit
CAATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCAC129363.038367136028937No Hit
AGCCTTCTCCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCA101342.380242158048643No Hit
CGCCCACGGGCTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACT97432.28840530351963No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC92232.1662693333020164No Hit
GGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCACGGTTAGGGT91122.1401980011978723No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA90062.115301053422743No Hit
GAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTACTAAGAAGA85292.003264788434663No Hit
CTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCAC81441.9128371951004686No Hit
TATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTT79301.8625735458186046No Hit
CTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTA77851.8285164002771546No Hit
TGCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGT77731.8256978778875177No Hit
CCTGCCCCTACTCCTCCTAGACCTAACCTGACTAGAAAAGCTATTACCTA76411.794694131601508No Hit
TTGGCTTGCCATGATTGTGAGGGGTAGGAGTCAGGTAGTTAGTATTAGGA73711.7312773778346704No Hit
TACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCT71871.6880600345268992No Hit
CATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACT69991.6439031837559162No Hit
ATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTA68101.5995114561191297No Hit
ACTGATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATG67901.5948139188030674No Hit
TAACCAATATTACCAATCAATACTCATCATTAATAATCATAATGGCTATA64541.515895291893225No Hit
GGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAG61331.440499817970429No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTAATATTGGTTATGGTTC60701.4257025754248336No Hit
GTCATTGGTGTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATT58441.3726204037533323No Hit
GGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAAT52991.2446125118906413No Hit
TTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGC51401.2072670902279479No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCA47691.120127773014997No Hit
CAGGTGGTCAAGTATTTATGGTACCGTACAATATTCATGGTGGCTGGCAG47481.1151953588331316No Hit
CATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAACGAA45921.0785545677678476No Hit
CCCCCTCTACCCCCTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACC45701.0733872767201793No Hit
AGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGG41130.9660485490481615No Hit
AAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGC39330.923770713203603No Hit
GGTGATAAGCTCTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTT33830.7945884370118965No Hit
TAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTAT31670.7438550339984263No Hit
AGGGGCAGGTTTTGGCTCGTAAGAAGGCCTAGATAGGGGATTGTGCGGTG31340.736104097426924No Hit
TACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCAT30500.7163744406994633No Hit
AACCCCCCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGG30260.7107373959201888No Hit
ATCAAAACACAAATGTGTTGATGGAGAGGCCTCAGGGGCCTTATCTGGGC21950.5155547204378105No Hit
ACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAGAAATTTAGGT19340.45425185846320065No Hit
TTTAAGTGCTGTGGCCAGAAGCGGGGGGAGGGGGGGGGTTTGGTGGAAAT12800.30064238822797146No Hit
TTTTAGCAAAATAGTAACTATTTTTGCTTAAATGAGATTGCACATGGATT9650.22665617549999412No Hit
GGCGATTAGCTCTTCTATGATAGGGGATGTGGCGTCTTGAAGACCTAGCT9600.22548179117097863No Hit
GATGATTAGTTCTTCTATGATAGGAGAAGTAGCGTCTTGTAGACCTACTT8640.20293361205388077No Hit
TCAGCCCCATTTTATTTTTTTAATTTATTTTTGAGACAGTGTCTCACTCT8050.1890758769714977No Hit
AATATTTTTTTATTATAGGAAATGGCTGAGTGTGGTGGCTCTGTAATCCC6170.1449190262005144No Hit
GGCCTTCTCCACTTCAAGCCAACTAGGACTTATAATAGTCACAATCGGCA5860.13763784336061818No Hit
CAGGTGGTCAAGTATTTATGGTACTGTACAATATTCATGGTGGCTGGCAG4530.10639922020880554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)