FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003034937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003034937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994555
Sequences flagged as poor quality0
Sequence length209-223
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT430984.333395337613304No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC387113.8922935383161312No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG323733.2550236035211726No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT318973.207163002548879No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG307953.0963596784491556No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG303273.0493034573251356No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT272112.7359975064224704No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC250662.520323159604044No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA250592.5196193272368044No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG246582.479299787342078No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG246512.4785959549748378No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT243692.450241565323185No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT239982.412938449859485No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT234532.3581400726958286No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC229962.3121898738631854No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT224242.254676714711605No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG219982.211843487791022No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT217502.1869077124945324No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA215552.1673009536928576No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG213232.1439739380929157No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG210502.1165244757705706No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC197271.9835001583622824No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC175861.7682280014679932No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA174981.759379823136981No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG168011.6892982288561218No Hit
GGGCTATTTAGGCTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACA166591.6750204865492606No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA165071.6597372694320576No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG161141.6202221093856046No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT146031.468294865542881No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC140481.4124910135688826No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC135641.3638260327483145No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG132451.3317513862983947No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG130771.314859409484644No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA130211.309228750546727No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT129271.2997772873295093No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT127531.2822820256295528No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA125721.264082931562357No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT125081.2576478927761663No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA117061.1770088129867127No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC107801.0839018455490144No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT96030.9655574603717241No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG93360.9387112829355843No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG92120.9262433952873396No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC91850.9235286132994153No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT72520.7291703324602461No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT67260.6762823574362403No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT53330.536219716355556No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG41040.41264686216448565No Hit
AATATTCTATCATCTGTAGGCTCATTTATTTCCCTAACAGCAGTAATACT25520.2565971715993585No Hit
AATATTTTATCATCTGTAGGCTCATTCATCTCCCTAAAAGCAGTAGTGCT24950.2508659651804073No Hit
TTTGTATAACCTAATATTTATGGACATCTATCAAATTATTAAAGACTTTT24840.24975994288903078No Hit
CTAGTGATGTGAAGTTCAGCAAATGTCTTCACTCCTCTGTGCCTCTGTTT19780.1988829174857097No Hit
TATTTTTGGGCTGTTGCTTTTTACTTGTCATTTTTGTGTTGTAAACCCTT16300.16389239408579717No Hit
CCTCCTCAAGTATACTTCAAAGGACACTTAACTAAAACCCCTACGCATTT12690.12759475343243962No Hit
TCAACTTAAACTCCAGCACCACAACCCTACTACTATCTCGCACCTGAAAC12590.12658927862209732No Hit
CGTAACCTCAACACCACCTTCTTCGACCCAGCCGGAGGAGGAGACCCCAT12170.1223662844186596No Hit
GTGCAAGGAGTTCAGTTATGTGTTTGGGATTTTTTAGATGACAGGTGTCG11620.11683617296177687No Hit
GGCTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT10620.10678142485835372No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)