FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003034957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003034957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48994
Sequences flagged as poor quality0
Sequence length208-223
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC31646.457933624525452No Hit
TTACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAG31056.337510715597829No Hit
ATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGAC28525.821120953586154No Hit
CATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGA22824.657713189370127No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC15583.179981222190472No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC15243.1105849695881127No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG15123.086092174551986No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT13952.847287422949749No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT13442.7431930440462096No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC11952.4390741723476346No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG11222.2900763358778624No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG10782.200269420745397No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT10662.1757766257092706No Hit
ATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCAAGC7151.4593623709025596No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG6981.4246642446013797No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG6651.3573090582520309No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT6361.298118136914724No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT5981.2205576193003225No Hit
ATGAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATGAC5801.1838184267461322No Hit
TTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATG5771.1776952279871005No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG5271.0756419153365717No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT5191.0593133853124872No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG4921.0042045964812019No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG4660.9511368739029269No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT4380.893987018818631No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT4290.8756174225415356No Hit
TTTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGAT4280.8735763562885251No Hit
CCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG4090.8347960974813242No Hit
TCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG4020.8205086337102503No Hit
TCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGACGTGCA3900.7960158386741233No Hit
GCGTGGTAAGGGCGATGAGACCTACGCACCGACCTACTGTAGATGACGTG3580.7307017185777851No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC3570.7286606523247745No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT3570.7286606523247745No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC3410.6960035922766052No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT3160.644976935951341No Hit
CATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGAC2970.60619667714414No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG2880.5878270808670449No Hit
TCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGA2860.5837449483610238No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG2710.5531289545658652No Hit
TTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCA2660.5429236233008123No Hit
GGTTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTG2480.506184430746622No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC2380.4857737682165163No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA2380.4857737682165163No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA2320.4735273706984529No Hit
ACATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG2270.4633220394334No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG2080.4245417806261992No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC2000.40821325060211455No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT1880.3837204555659877No Hit
TCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCAA1740.35514552802383964No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA1730.3531044617708291No Hit
ACATCTCTGCCAAACCCCAAACCTACGCACCGACCTACTGTAGATGACGT1650.3367759317467445No Hit
GTGGTAAGGGCGATGAGACCTACGCACCGACCTACTGTAGATGACGTGCA1630.33269379924072334No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA1550.31636526921663877No Hit
TTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGCAAGCT1420.28983140792750134No Hit
TTACCTCACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTAC1390.2837082091684696No Hit
TGGTAAGGGCGATGAGACCTACGCACCGACCTACTGTAGATGACGTGCAA1340.27350287790341676No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT1310.267379679144385No Hit
GGGTTTTGCAGTCCTTAGCTGTTACAGAAATTAAGTATTGCAACTTACTG1220.24901008286728987No Hit
CAATGAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATG1200.24492795036126871No Hit
CGTGGTAAGGGCGATGAGACCTACGCACCGACCTACTGTAGATGACGTGC1200.24492795036126871No Hit
TTACTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGA1200.24492795036126871No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA1170.23880475160223702No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA1150.2347226190962159No Hit
TCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGATGAC1140.23268155284320527No Hit
TGAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATGACG1130.2306404865901947No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA1080.22043515532514185No Hit
AATGAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATGA1070.2183940890721313No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1030.21022982406008897No Hit
TGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGACGT1020.2081887578070784No Hit
ATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGATGACG1000.20410662530105728No Hit
ATTTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGA950.19390129403600442No Hit
GTAAGGGCGATGAGACCTACGCACCGACCTACTGTAGATGACGTGCAAGC940.19186022778299386No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG930.18981916152998327No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG920.1877780952769727No Hit
CCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGACGTGC850.17349063150589866No Hit
ACGACCCCAATACGCAAAACTAACCCCACCTACGCACCGACCTACTGTAG820.16736743274686697No Hit
CTCCTCCTAGACCTAACCTGACACCTACGCACCGACCTACTGTAGATGAC800.16328530024084584No Hit
GAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACTGTAGATGACGT780.15920316773482468No Hit
TCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGACG740.15103890272278236No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT740.15103890272278236No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG730.1489978364697718No Hit
ACGACCCAATACGCAAAACTAACCCCACCTACGCACCGACCTACTGTAGA720.14695677021676123No Hit
AAGATATCAATGAGGAGTAGGAGGTTGGCCAACCTACGCACCGACCTACT690.14083357145772954No Hit
GTTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGTGC690.14083357145772954No Hit
ACTCCTCCTAGACCTAACCTGACACCTACGCACCGACCTACTGTAGATGA650.13266930644568722No Hit
TGGTTCATTGTCCGGAGAGTACCTACGCACCGACCTACTGTAGATGACGT630.12858717393966607No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC610.12450504143364494No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT570.11634077642160263No Hit
AAAGGGAGCACCAAGTATAGCACAAAACCCAGACCCCCCGATCCAAGCAG550.1122586439155815No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT550.1122586439155815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)