FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003035303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003035303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298200
Sequences flagged as poor quality0
Sequence length208-223
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT159975.3645204560697515No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT148434.977531857813548No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT122184.097250167672703No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC112223.763246143527834No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC95813.2129443326626426No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT93843.146881287726358No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG91963.083836351441985No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT88352.9627766599597587No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC88332.9621059691482228No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG83452.7984574111334677No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT79892.6790744466800804No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG77952.6140174379611003No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT70242.355466130114017No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT67852.2753185781354794No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG67512.2639168343393696No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG67112.250503018108652No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC66712.237089201877934No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC66522.2307176391683434No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG65502.1965124077800136No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA61562.0643863179074446No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG59601.9986586183769282No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG57581.9309188464118041No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG56691.9010731052984575No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG54061.812877263581489No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT50851.7052313883299797No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT49981.676056338028169No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC47201.5828303152246814No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC47051.5778001341381624No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC46711.5663983903420522No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA45321.5197853789403086No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT44051.47719651240778No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC43981.4748490945674044No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT42631.4295774647887325No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA42411.4221998658618376No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA40051.3430583501006037No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA38341.2857142857142856No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA37861.2696177062374245No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG35541.1918175720992623No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT33181.112676056338028No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA32421.0871898054996645No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT26030.8729040912139503No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG22910.7682763246143528No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT20280.6800804828973843No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG19400.6505700871898055No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG16490.5529845741113347No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG11470.3846411804158283No Hit
GGGCTATTTAGGCTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACA9440.3165660630449363No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT3580.12005365526492287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)