FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003035431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003035431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373965
Sequences flagged as poor quality0
Sequence length210-223
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT177034.733865468693595No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC171174.577166312355434No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG163524.37260171406415No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG140423.754896848635567No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG135193.6150441886272784No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT134163.587501504151458No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG116203.1072426563983258No Hit
TTACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAG112273.0021526078643723No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT95542.5547845386600354No Hit
AATCCTAGGCCTACCCGCCACAGTACTGATCATTCTATTTCCCCCTCTAT93432.4983621461901513No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT90322.415199283355394No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC75062.0071397055874214No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG73561.9670290000401107No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT70721.8910860642038694No Hit
GGGCTATTTAGACTTTATGGACCTGAAGTAGGAACCAGATGTCGGATACA67701.8103298437019508No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT67381.8017728931851913No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG66741.784658992151672No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT66481.777706469856805No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG66361.77449761341302No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA64081.713529340981108No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG63931.709518270426377No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA62701.676627491877582No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT61891.6549677108820344No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG60291.6121829582982365No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG55611.4870375569906276No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG53371.4271389033733104No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA53171.4217908093003357No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC51751.3838193413822149No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC50961.3626943697939646No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA48791.3046675491021886No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC48731.3030631208802963No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC47721.2760552458117738No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA46451.242094848448384No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT45201.208669260492292No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT42441.1348655622852406No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA41581.1118687577714492No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA38951.0415413207118314No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT36150.9666680036901848No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG35740.9557044108405867No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG33840.9048975171473267No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT33010.8827029267444815No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC29160.7797521158397176No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT28830.7709277606193092No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG25980.6947174200794192No Hit
ATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGAC25750.6885671118954982No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT25700.6872300883772545No Hit
CATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGA25360.6781383284531974No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC24070.6436431216825104No Hit
ATAAAGTCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG21670.5794659928068135No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC12410.33184923722808285No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT11300.302167315123073No Hit
AATATTCTATCATCTGTAGGCTCATTTATTTCCCTAACAGCAGTAATACT7500.20055352773655286No Hit
TTTGTATAACCTAATATTTATGGACATCTATCAAATTATTAAAGACTTTT6930.18531145962857487No Hit
AATATTTTATCATCTGTAGGCTCATTCATCTCCCTAAAAGCAGTAGTGCT6880.18397443611033118No Hit
TTACCTCACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTAC6650.17782412792641022No Hit
ACGACCCCAATACGCAAAACTAACCCCACCTACGCACCGACCTACTGTAG6330.16926717740965064No Hit
CCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG5590.14947922933964408No Hit
TTACTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGA5510.1473399917104542No Hit
TCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG4810.12862166245504258No Hit
CTAGTGATGTGAAGTTCAGCAAATGTCTTCACTCCTCTGTGCCTCTGTTT4580.12247135427112163No Hit
TATTTTTGGGCTGTTGCTTTTTACTTGTCATTTTTGTGTTGTAAACCCTT4390.11739066490179563No Hit
ACGACCCAATACGCAAAACTAACCCCACCTACGCACCGACCTACTGTAGA4280.11444921316165953No Hit
GGCTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT4140.1107055473105772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)