FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003035841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003035841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192057
Sequences flagged as poor quality0
Sequence length209-223
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC95434.96883737640388No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG80674.200315531326638No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG76153.964968733240652No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT76093.961844660699688No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG63393.30058263952889No Hit
GAGGGTGAACTCACTGGAATGGGGATGCTTGCATGTGTAATCTTACTAAG59863.1167830383688178No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT59853.11626235961199No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG56752.954851944995496No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT55642.8970566029876545No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT52592.738249582155298No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT46602.426363006815685No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG45032.344616441993783No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG43592.2696387010106376No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT43112.2446461206829222No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC43002.238918654357821No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG41792.1759165247817056No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT41662.169147700942949No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA40622.1149971102328995No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT40282.097294032500768No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA37771.9666036645370903No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA37521.9535866956164056No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG34111.776035239538262No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA33171.7270914363964864No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG32631.6989747835278068No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG31731.652113695413341No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC31241.6266004363287982No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA29991.5615155917253731No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT29711.5469365865342062No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT27941.4547764465757562No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA26911.4011465346225338No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC26441.3766746330516462No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT24221.2610839490359633No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG23731.2355706899514207No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC21871.1387244411815243No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA21291.1085250732855352No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT21021.094466746851195No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT17330.9023362855818845No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT16910.8804677777951337No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC16660.8674508088744487No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA16570.8627647000630021No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT16140.8403755135194239No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT14880.7747699901591715No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC13840.7206193994491219No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG13790.7180160056649848No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG12970.675320347605138No Hit
TCAACTTAAACTCCAGCACCACAACCCTACTACTATCTCGCACCTGAAAC11960.6227317931655706No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG10090.5253648656388469No Hit
AATATTCTATCATCTGTAGGCTCATTTATTTCCCTAACAGCAGTAATACT6380.3321930468558813No Hit
AATATTTTATCATCTGTAGGCTCATTCATCTCCCTAAAAGCAGTAGTGCT6170.3212587929625059No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC6140.3196967566920237No Hit
CTAGTGATGTGAAGTTCAGCAAATGTCTTCACTCCTCTGTGCCTCTGTTT5300.2759597411185221No Hit
TTTGTATAACCTAATATTTATGGACATCTATCAAATTATTAAAGACTTTT5180.2697115960365933No Hit
GGCTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT3060.15932769958918447No Hit
CCTCCTCAAGTATACTTCAAAGGACACTTAACTAAAACCCCTACGCATTT2900.15099683947994605No Hit
TATTTTTGGGCTGTTGCTTTTTACTTGTCATTTTTGTGTTGTAAACCCTT2860.14891412445263644No Hit
GTGCAAGGAGTTCAGTTATGTGTTTGGGATTTTTTAGATGACAGGTGTCG2420.12600425915223085No Hit
ATTAAGTTACTAGCTCAGGAGTTTGATAGCTCTTGGGCAGTAAGGGTGAG2120.11038389644740884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)