FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003036380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003036380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642202
Sequences flagged as poor quality0
Sequence length223-233
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTA299724.667067371325533No Hit
CCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT247323.8511247239964996No Hit
CACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCA246813.8431832974671525No Hit
GACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT246303.835241870937805No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA245353.8204490175988237No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTA237353.6958776210600406No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACT228233.553866229005827No Hit
CTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG226993.5345576625423156No Hit
GATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT207463.23044774074201No Hit
CAATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCAC198213.086412063494041No Hit
TAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGA172282.6826450244627082No Hit
AGCCTTCTCCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCA150682.3463022538079916No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC147682.299587980105948No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTAGTATTGGTTATGGTTC143722.23792513881925No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA138532.157109445314714No Hit
CGCCCACGGGCTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACT138332.1539951604012444No Hit
TATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTT131642.0498223300456866No Hit
CCTGCCCCTACTCCTCCTAGACCTAACCTGACTAGAAAAGCTATTACCTA125321.9514109267800475No Hit
TTGGCTTGCCATGATTGTGAGGGGTAGGAGTCAGGTAGTTAGTATTAGGA124841.9439366429877203No Hit
CTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTA124831.9437809287420469No Hit
GGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCACGGTTAGGGT117421.8283966726979985No Hit
TGCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGT113671.7700038305704435No Hit
TACTTGACCACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCT108411.6880981373461932No Hit
GTCATTGGTGTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATT104091.6208295832152502No Hit
CATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACT104031.619895297741209No Hit
GAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTACTAAGAAGA101571.581589593305533No Hit
CTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATCAC99921.555896742769409No Hit
ACTGATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATG97721.5216396087212436No Hit
ATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGTCATTA95531.4875381889187513No Hit
TAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATA93691.4588867677148312No Hit
GGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAAT91891.4308582034936048No Hit
TTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGC89581.394888212743031No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCA82311.2816839561384112No Hit
CATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAACGAA73161.1392054213471774No Hit
GGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAG71271.1097754289148898No Hit
AAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGC68791.0711582959878667No Hit
CAGGTGGTCAAGTATTTATGGTACCGTACAATATTCATGGTGGCTGGCAG68051.0596354418080294No Hit
CCCCCTCTACCCCCTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACC66861.0411054465728853No Hit
TTTAAGTGCTGTGGCCAGAAGCGGGGGGAGGGGGGGTTTGGTGGAAATTT65131.0141668820713732No Hit
AGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGG62420.9719683214938601No Hit
TAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTAT55730.8677954911383022No Hit
AGGGGCAGGTTTTGGCTCGTAAGAAGGCCTAGATAGGGGATTGTGCGGTG52140.811894076941523No Hit
AACCCCCCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGG52130.8117383626958496No Hit
TACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCAT46440.7231369569076397No Hit
GGTGATAAGCTCTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTT44820.697911249108536No Hit
TCCCCCAGGCTCCTGGCAGCTCTAGGCAATATCAAGCCTCCTAGAGAGGA33930.5283384355701166No Hit
ATCAAAACACAAATGTGTTGATGGAGAGGCCTCAGGGGCCTTATCTGGGC31530.49096701660848147No Hit
ACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAGAAATTTAGGT18890.2941442100772031No Hit
GGCGATTAGCTCTTCTATGATAGGGGATGTGGCGTCTTGAAGACCTAGCT13650.21254994534429977No Hit
TTTTAGCAAAATAGTAACTATTTTTGCTTAAATGAGATTGCACATGGATT9820.15291138925135705No Hit
TCAGCCCCATTTTATTTTTTTAATTTATTTTTGAGACAGTGTCTCACTCT8670.1350042509989069No Hit
AATATTTTTTTATTATAGGAAATGGCTGAGTGTGGTGGCTCTGTAATCCC7410.11538425604404844No Hit
AATAAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC7220.11242568537625233No Hit
GGCCTTCTCCACTTCAAGCCAACTAGGACTTATAGTAGTCACAATCGGCA6850.10666425828633358No Hit
GCCGGCCTCCACTATAGCAGATGCAAGCAGGAGTAGGAGAGAGGGGGGTA6670.10386140186421096No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTGGTATTGGTTATGGTTC6590.1026156878988231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)