FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003036565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003036565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248087
Sequences flagged as poor quality0
Sequence length208-223
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT161966.528354972247639No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG136995.521853220845913No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT125285.049841386287874No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG110114.438362348692192No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG93693.776497760866148No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA89553.609620818503186No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT87403.5229576721069624No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA85833.459673421017627No Hit
GGACATAGCCTATGAAGGCTGTTGCTATGGTTGCAAGCAGGAGGATAATG81243.2746576805717345No Hit
GAGGGTGAACTCACTGGAGTGGGGATGCTTGCATGTGTAATCTTACTAAG81183.2722391741606773No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC81013.2653867393293483No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT72472.92115266015551No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG70012.821993897302156No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC67252.7107426023935153No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG65042.621660949586234No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG62432.5164559207052366No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT59672.4052046257965953No Hit
CGCAACCTTAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT56792.28911631806584No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT53162.1427966801968665No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA44991.8134767238912157No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG44581.7969502634156567No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA43821.7663158488755961No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG42511.713511792234176No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG39541.5937957248868342No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA36751.4813351767726646No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT35171.417647841281486No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT34771.4015244652077699No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG34591.3942689459745976No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT31461.2681035281977693No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT31301.261654177768283No Hit
AGTTGAGTAGTAGGAATGCGGTGGTAGTTAGGATAATATAAATAGTTAAA30721.2382752824613945No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATTAGGGCGTAGTT29381.1842619726144457No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAACA27931.1258147343472251No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG22090.8904134436709702No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT21370.8613913667382813No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC20450.8243076017687343No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC19270.7767436423512719No Hit
AATATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT18810.7582017598664985No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA16370.6598491658168304No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT16050.6469504649578575No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC13350.538117676460274No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAGTG11930.480879691398582No Hit
ACCATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC11100.4474236860456211No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC10870.43815274480323435No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG7930.3196459306614212No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCTACCCTTACTAAC7150.28820534731767483No Hit
CGCAACCTTAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAC6810.2745004776550162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)