FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003036621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003036621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222922
Sequences flagged as poor quality0
Sequence length210-223
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG133245.976978494720126No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT101554.55540502956191No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG96504.328868393429092No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG94724.249019836534752No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG90384.054332905680013No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC88533.971344236997694No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT86973.9013646028655766No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG83613.750639237042553No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT74283.332107194444694No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT72173.2374552534070213No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG70123.1454948367590454No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA63852.864230538035726No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA62912.822063322597142No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG60272.70363624945048No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC59312.660571859215331No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG58132.60763854621796No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT57302.5704057921604866No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT54292.435380985277362No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT51482.309327926359893No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG44451.993970985367079No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA43941.971093028054656No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG40621.822162011824764No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA38751.738276168345879No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT35071.5731960057778056No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA33331.4951417984765971No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG32011.4359282619032667No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT31541.4148446541839748No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA30001.3457621948484222No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA26951.208943038372166No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT25941.1636357111456026No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT22331.001695660365509No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT21520.9653600811046017No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC20050.8994177335570289No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC19730.885062936811979No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG19130.8581476929150107No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG18990.8518674693390513No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC18760.8415499591785468No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA17220.7724674998429943No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT16500.7401692071666323No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC14190.6365455181633037No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG13510.6060415750800728No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT12530.5620800100483577No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC11920.5347161787531064No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT11740.526641605584016No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC10900.48896026412826016No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC7460.3346461991189743No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCAGTTTCATCCTCGCCTT5990.2687038515714017No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG3360.1507253658230233No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)