FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003037141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003037141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306380
Sequences flagged as poor quality0
Sequence length208-223
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG3188310.406358117370585No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC168355.494810366211893No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG148204.837130360989621No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT127924.175207258959462No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT117603.838370650825772No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT115403.766564397153861No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG98853.2263855343038057No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT95393.113453880801619No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG93963.066779815914877No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT93443.0498074286833345No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA88012.872576538938573No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT83542.7266792871597363No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG74982.4472876819635747No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA74062.41725961224623No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC73292.3921274234610617No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT66342.1652849402702525No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC64582.107839937332724No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG60721.9818526013447353No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG53641.750767021346041No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA52071.6995234675892683No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA47731.5578693126183172No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG46901.530778771460278No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC44621.456361381291207No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT40851.3333115738625236No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT39241.2807624518571707No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC39121.2768457471114303No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT38901.2696651217442392No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA37701.2304980742868332No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC37351.2190743521117566No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA36261.1834976173379463No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC35951.1733794634114498No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT35411.1557542920556172No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG35261.1508584111234414No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG33821.1038579541745543No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG30701.0020236307852992No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT29930.9768914420001307No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG28860.9419674913506103No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT25890.845029048893531No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA25470.8313205822834389No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT24660.8048828252496899No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC23690.7732227952216202No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC22780.7435211175664208No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA22650.7392780207585351No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT13820.45107382988445716No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG8870.28950975912265814No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG4760.15536262158104314No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCA3140.10248710751354527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)