FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003037245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003037245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140243
Sequences flagged as poor quality0
Sequence length210-223
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAG103147.354377758604707No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG46803.337064951548384No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC42473.028315138723501No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT41192.93704498620252No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA40492.887131621542608No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT37882.7010260761677944No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG34372.450746204801666No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG32772.336658514150439No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT32472.315267072153334No Hit
CATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGA29922.1334398151779412No Hit
ATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATGAC28892.059995864321214No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT28052.0000998267293197No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT26991.9245167316728822No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT26161.8653337421475582No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC25081.7883245509579802No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG24111.7191588885006739No Hit
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG22711.6193321591808505No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC22371.5950885249174647No Hit
TAGGATTGGTGCTGTGGGTGAAAGAGTATGATGGGGTGGTGGTTGTGGTA22261.5872449961851929No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA21741.550166496723544No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT21581.5387577276584214No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG21521.5344794392590004No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA21451.5294881027930092No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG20881.4888443629985098No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA20681.4745834016671062No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC20631.4710181613342554No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT20101.4332266138060368No Hit
GCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTG19771.4096960276092212No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT19481.3890176336786864No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT18671.3312607402865027No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG18631.3284085480202221No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG18541.3219911154210906No Hit
GTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTG17281.2321470590332495No Hit
TTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAAT16221.1565639639768115No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG16101.1480073871779697No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT15821.1280420413140049No Hit
ATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGG15571.1102158396497508No Hit
ACTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATC15101.076702580520953No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC14991.0688590517886811No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC13420.9569105053371647No Hit
AACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATT12430.8863187467467183No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA12210.8706316892821746No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA12110.863501208616473No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA10660.7601092389637986No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG10370.7394308450332637No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTT9700.6916566245730625No Hit
GCAGTCTCTAGCAGCTGTCTTCCCAAACGGGTGTGTGCCTGCAAAGCCGG9210.6567172693111243No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT8190.5839863665209671No Hit
CCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG7170.5112554637308101No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC6700.4777422046020123No Hit
ACGACCCCAATACGCAAAACTAACCCCACCTACGCACCGACCTACTGTAG5320.37934157141532915No Hit
TTACCTCACCTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTAC5240.37363718688276776No Hit
TCATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG5230.37292413881619757No Hit
TCTCTGAAAATATAAATTTTAGATGGCTGTTGACTTATCTTGAAAATGGT4900.3493935526193821No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC4090.2916366592271985No Hit
AATATTTTATCATCTGTAGGCTCATTCATCTCCCTAAAAGCAGTAGTGCT4050.28878446696091786No Hit
ACATCGCCACTTATCCAGTGAACCACCTACGCACCGACCTACTGTAGATG3890.2773756978957952No Hit
TTACTCCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGA3700.26382778463096196No Hit
AATATTCTATCATCTGTAGGCTCATTTATTTCCCTAACAGCAGTAATACT3200.22817538130245357No Hit
ACGACCCAATACGCAAAACTAACCCCACCTACGCACCGACCTACTGTAGA3080.2196188045036116No Hit
TTTGTATAACCTAATATTTATGGACATCTATCAAATTATTAAAGACTTTT2790.1989404105730767No Hit
CTAGTGATGTGAAGTTCAGCAAATGTCTTCACTCCTCTGTGCCTCTGTTT2610.18610554537481372No Hit
TTTCATGATCACGCCCTCATAATCAACCTACGCACCGACCTACTGTAGAT2280.1625749591779982No Hit
TTACCTCACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGA2020.14403570944717384No Hit
TATTTTTGGGCTGTTGCTTTTTACTTGTCATTTTTGTGTTGTAAACCCTT1870.13333998844862133No Hit
CGTAACCTCAACACCACCTTCTTCGACCCAGCCGGAGGAGGAGACCCCAT1790.12763560391606No Hit
TTACTTCAAGTCAACTAGGACACCTACGCACCGACCTACTGTAGATGACG1540.1098094022518058No Hit
ATTAAGTTATTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTAAGAGTGAG1520.10838330611866547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)