FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003037304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003037304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146
Sequences flagged as poor quality0
Sequence length223-233
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGG96.164383561643835No Hit
TAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGGAGA85.47945205479452No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTA85.47945205479452No Hit
CAGTCTCTTCCCCACAACAATATTCATGTGCCTAGACCAAGAAGTTATTA85.47945205479452No Hit
GATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT74.794520547945205No Hit
ACTCCTACCTATCTCCCCTTTTATACTAATAATCTTATAGAAATTTAGGT64.10958904109589No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTC64.10958904109589No Hit
TCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAA53.4246575342465753No Hit
CATTATGATTATTAATGATGAGTATTGATTGGTAGTATTGGTTATGGTTC53.4246575342465753No Hit
GTCATTGGTGTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATT53.4246575342465753No Hit
AGCCTTCTCCACTTCAAGTCAACTAGGACTCATAATAGTTACAATCGGCA53.4246575342465753No Hit
CATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACT42.73972602739726No Hit
TTGGCTTGCCATGATTGTGAGGGGTAGGAGTCAGGTAGTTAGTATTAGGA42.73972602739726No Hit
CAGGTGGTCAAGTATTTATGGTACCGTACAATATTCATGGTGGCTGGCAG42.73972602739726No Hit
GACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCT32.054794520547945No Hit
CCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAA32.054794520547945No Hit
CTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTA32.054794520547945No Hit
ACTGATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATG32.054794520547945No Hit
CAATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCAC21.36986301369863No Hit
CCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT21.36986301369863No Hit
TCCGTGGGCGATTATGAGAATGACTGCGCCGGTGAAGCTTCAGGGGGTTT21.36986301369863No Hit
CGCCCACGGACTTACATCCTCATTACTATTCTGCCTAGCAAACTCAAACT21.36986301369863No Hit
CAATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCAC21.36986301369863No Hit
AGGGGCAGGTTTTGGCTCGTAAGAAGGCCTAGATAGGGGATTGTGCGGTG21.36986301369863No Hit
CATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAACGAA21.36986301369863No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACT21.36986301369863No Hit
AAGGCTACGATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGC21.36986301369863No Hit
CACTTAAACACATCTCTGCCAAACCCCAAAAACAAAGAACCCTAACACCA21.36986301369863No Hit
GAGGGCAATTTCTAGATCAAATAATAAGAAGGTAATAGCTACTAAGAAGA21.36986301369863No Hit
GGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGAACGGTTAGGGT10.684931506849315No Hit
TACTTGATCACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCT10.684931506849315No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCTCTCATAATCATTTT10.684931506849315No Hit
TAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTAT10.684931506849315No Hit
CCCCCTCTACCCCCTCTAGAGCCCACTGTAAAGCTAACTTAGCATTAACC10.684931506849315No Hit
GATTTAACTGTTGAGGTTTAGGACTAAGCATAGTGGGGTATCTAATCCCA10.684931506849315No Hit
ACTGATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGCATG10.684931506849315No Hit
GGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCACGGTTAGGGT10.684931506849315No Hit
TATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTT10.684931506849315No Hit
TAACCAATACTACCAATCAATACTCATCATCAATAATCATAATGGCTATA10.684931506849315No Hit
CATCTTTCTTTTTACCGTAGGTGGCCTGACTGGCATTGTATTAGCAAACT10.684931506849315No Hit
TACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACCAT10.684931506849315No Hit
TGCGATTAGAATGGGTACAATGAGGAGTAGGAGGTTGGCCATGGGTATGT10.684931506849315No Hit
TACTTGGCCACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCT10.684931506849315No Hit
AACCCCCCTCCTAATACTAACTACCTGACTCCTACCCCTCACAATCATGG10.684931506849315No Hit
GATTTAACTGTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCA10.684931506849315No Hit
TAGAAGTAGGGTCTTGGTGACAAAATATGTTGAGTATAGTTCAGGGAAGA10.684931506849315No Hit
CTTGCGATGCTCTCTGTGGCCGGGGCACCTACGGCATCGCCACTCATCGT10.684931506849315No Hit
TAACCAATACTACCAATCAATACTCATCATTAATAATCATAATGGCTATA10.684931506849315No Hit
GATAAGGGGCGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTT10.684931506849315No Hit
TCCGGCCTCCACTATAGCAGATGCGAGCAGGAGAAGGAGAGAGGGAGGTA10.684931506849315No Hit
GGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAAT10.684931506849315No Hit
CAGGTGGTCAAGTATTTATGGTACCGTGCAATATTCATGGTGGCTGGCAG10.684931506849315No Hit
CACTATGATTATTAATGATGAGTATTGATTGGTAGTATTGGTTATGGTTC10.684931506849315No Hit
ATTGTCTGGGTCGCCTAGGAGGACTGGTGAGAATAGTGTTAAGGTCATTA10.684931506849315No Hit
AGGGGCAGGTTTTGGCTCGCGAGAAGGCCTAGATAGGGGATTGTGCGGTG10.684931506849315No Hit
TTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGC10.684931506849315No Hit
AGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGAGTTTTAGGGG10.684931506849315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)