FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003037305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003037305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146
Sequences flagged as poor quality0
Sequence length211-220
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCG85.47945205479452No Hit
TCGCCGATACGGTTGTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATC74.794520547945205No Hit
CGAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT74.794520547945205No Hit
CCTCCTCAAGTATACTTCAAAGGACATTTAACTAAAACCCCTACGCATTT74.794520547945205No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTAT64.10958904109589No Hit
AAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAG64.10958904109589No Hit
CGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACCCCAT64.10958904109589No Hit
AAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCT53.4246575342465753No Hit
AATCCTACCTCCATCGCTAACCCCACTAAAACACTCACCAAGACCTCAAC53.4246575342465753No Hit
GGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGCGCTTGT53.4246575342465753No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCAGCTAAATAA42.73972602739726No Hit
ACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTTTGCCGCC42.73972602739726No Hit
GCGAAGGCTTCTCAAATCATGAAAATTATTAATATTACTGCTGTTAGAGA42.73972602739726No Hit
GGACATAGCCTATGAAGGCTGTTGCTATAGTTGCAAGCAGGAGGATAATG42.73972602739726No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGT42.73972602739726No Hit
CCTATCCCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATA32.054794520547945No Hit
AACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGCTTTTAT32.054794520547945No Hit
TATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAGGAGGG32.054794520547945No Hit
TAATTCTTCTCACCCTAACAGGTCAACCTCGCTTCCCCACCCTTACTAAC32.054794520547945No Hit
TATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATG32.054794520547945No Hit
CACCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCA21.36986301369863No Hit
TTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTT21.36986301369863No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTATTTAGCTGACCTTACTTTAGG21.36986301369863No Hit
TATGAGATTGTTTGGGCTACTGCTCGCAGTGCGCCGATCAGGGCGTAGTT21.36986301369863No Hit
AAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATC21.36986301369863No Hit
ATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTG21.36986301369863No Hit
TTGTTGATGAGATATTTGGAGGTGGGATCAATAGAGGGGGGAATAGAATG10.684931506849315No Hit
GCCTAGCCATGTGATTTCACTTCCACTCCATAACGCTCCTCATACTAGGC10.684931506849315No Hit
TCAGGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGC10.684931506849315No Hit
AGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATT10.684931506849315No Hit
CCTCCTCAAGTATACTTAAAAGGACATTTAACTAAAACCCCTACGCATTT10.684931506849315No Hit
CCTCCTCAAATATACTTCAAAGGACATTCAACTAAAACCCCTACGCATTT10.684931506849315No Hit
TCTCCGTGCCACCTATCACACCCCATCCTAAAGTAAGGTCACCTAAATAA10.684931506849315No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAG10.684931506849315No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGATAGGATAATATAAATAGTTAAA10.684931506849315No Hit
AATACTTCACTCGGCCATTTTACCTCACCCCCACTGATGTTGGCCGTCCG10.684931506849315No Hit
CAAGCAATCCTATACAACCGTATCGGCGATATCGATTTCATCCTCGCCTT10.684931506849315No Hit
ACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACT10.684931506849315No Hit
CAAGCAATCCTATACAACCGTATCGGCGCTATCGGTTTCATCCTCGCCTT10.684931506849315No Hit
TGAATCGGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACATGT10.684931506849315No Hit
GCGAAGGCTTCTCAAATCATTAAAAATATCAATGTTAGTGCTGTTAGAGA10.684931506849315No Hit
AATCCTAGGCCTACCCGCCGCAGTACTGTTCATTCTATTTCCCCCTCTCT10.684931506849315No Hit
AGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATAAATAGTTAAA10.684931506849315No Hit
AGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCTGTTGGTCAAG10.684931506849315No Hit
CCTATCCCTCAACCCCGACATAATTAGCATGTTTTCCTCTTTTAAATATA10.684931506849315No Hit
TCTCCGTGCCACCTATCACACTCCATCCTAAAGTAAGGTCAGCTAAATAA10.684931506849315No Hit
TGCAGCCTTCATGGTTTCGACCTACGCACCGACCTACTGTAGATGACGTG10.684931506849315No Hit
GAGGGTGAACCCACTGGAATGGGGATGCTTGCATGTGTAATCTTACTAAG10.684931506849315No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGGTCTAG10.684931506849315No Hit
AGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACA10.684931506849315No Hit
CCTATAGCAACAGCCTTCATAGGCTATGTCCTCCCGTGAGGCGAAATTTC10.684931506849315No Hit
AGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAG10.684931506849315No Hit
CATTTTCGGGGTATGGGCCCGATAGCTTCTTTAGCTGACCTTACTTTAGG10.684931506849315No Hit
CTAGCAGTAGCCCAAACAATCTCATATGAAGTCACCCTAGCCATCATTCT10.684931506849315No Hit
TCAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAAC10.684931506849315No Hit
ATTAAGTTGTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAG10.684931506849315No Hit
CGAGCAACAGCCCAGACAATCTCACCTGAAGTCACCCTAGCCATCATTCT10.684931506849315No Hit
GAGGGTGAACTCACTGGAACGGGGATGCTTGCATGTGTAATCTTACTAAG10.684931506849315No Hit
AATCCTAGGCCTACCAGCCGCAGTACTGATCATTCTATTTCACCCTCTAT10.684931506849315No Hit
GGGCTATTTAGGCTTTATGGCCCTGAAGTAGGAACCAGATGTCGGATACA10.684931506849315No Hit
AACTCCTGATCCACCAACTTAACATGACTAGCTTACACAATCGCTTTTAT10.684931506849315No Hit
GGGCTATTTAGGCTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACA10.684931506849315No Hit
TCGCCGATACGGTTGTATAGGATTGATTGAATGGATGCTGTGTTGGCATC10.684931506849315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)