FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003057925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24060497
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA12967485.3895312303814835No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA9336313.880347941274862No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG8491843.529370153908292Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT5075612.109520015318054Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT4486711.864761978940003Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT4293421.7844269800411854Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC3194331.3276242797478373No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA2831181.1766922354097673No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2701761.1229028228303013Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA2591101.0769104229226854No Hit
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC2469361.0263129643581343Illumina RNA PCR Primer (100% over 32bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT2371460.9856238630482156Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC2246990.9338917645799253Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT2041970.8486815546661401Illumina RNA PCR Primer (100% over 31bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA2010930.8357807405225255No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1976630.8215250083986212Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1898030.7888573540272257Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA1712080.7115729986791213No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA1708590.7101224883259893No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT1379590.5733838332599697No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT1289460.535924091676078Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC1237810.5144573696877499Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1217610.5060618656381038Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG1186490.49312780197350037Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA1142790.47496525113342425No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT1096870.4558800260859117Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA1055220.43856949422117086No Hit
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG1021050.42436779256887336Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC932940.38774760138994635Illumina RNA PCR Primer (100% over 39bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG902420.37506290913275814Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC900650.3743272634808832Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA876460.3642734395719257Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT843900.3507408845295257Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA797550.3314769433066989No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT765910.31832675775566893Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG757970.3150267427975407No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA748250.31098692599741395No Hit
AGGTTTCAATTTCTATCGCCTATGATCGTCGGACTGTAGAACTCTGAACG729180.3030610714317331Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG708080.29429151027096406Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA679490.2824089627076282No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT672090.27933338201617364Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA618200.2569356734401621Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG607630.25254258047953043Illumina DpnII expression Sequencing Primer (96% over 29bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT590340.2453565277558481Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG590100.245256779192882Illumina DpnII expression Sequencing Primer (96% over 26bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT584590.24296671843478546Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA583180.24238069562735964No Hit
AGCGACGCTCAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT577590.24005738534827442Illumina RNA PCR Primer (100% over 31bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA573190.238228661693896No Hit
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA563150.23405584680981445Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG532580.2213503736020083Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG527810.2193678709130572Illumina DpnII expression Sequencing Primer (95% over 24bp)
TGGTATTCTCGCACGGACTACAACCACGATCGTCGGACTGTAGAACTCTG521180.21661231686111887Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT517270.21498724652279627Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA504500.2096797917349754No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC503770.20937638985595353Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT503320.20918936130039212Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG495010.2057355673076911Illumina DpnII expression Sequencing Primer (96% over 29bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG492340.20462586454469334Illumina RNA PCR Primer (100% over 33bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC481030.19992521351491618Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT461480.19179986182330316Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG460430.1913634618603265Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC453800.18860790780838818Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC443080.18415247199590265No Hit
AGCGACGCTCAGACAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT432750.17985912759823705Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG431660.17940610287476605Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG431480.17933129145254148Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA424670.17650092597837858No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT414880.17243201584738668Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC413000.17165065210415228No Hit
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA392510.1631346185409221No Hit
AACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGT391970.16291018427424836Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAG386570.16066584160751127Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT384190.15967666835809752Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT379610.15777313328149456Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT371120.154244527866569Illumina RNA PCR Primer (100% over 38bp)
ACAAACCCTTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG370160.15384553361470463Illumina RNA PCR Primer (100% over 34bp)
AAGCCAGTCAAATTTAGCGATCGTCGGACTGTAGAACTCTGAACGTGTAG369320.1534964136443233Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGTGGATCTCGGACTGTAGAACTCTGAACGTGTAGATC363860.1512271338368447Illumina RNA PCR Primer (100% over 30bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG356820.14830117598983927Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGTG351720.1461815190268098Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT349610.1453045629107329Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG339620.14115252897726926Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT337020.14007191954513656Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG335010.13923652533029554Illumina DpnII expression Sequencing Primer (96% over 29bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA334980.13922405675992477Illumina RNA PCR Primer (100% over 45bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG324740.13496811807337145Illumina DpnII expression Sequencing Primer (96% over 29bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT316950.13173044596709702Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG315980.13132729552510905Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT297210.12352612666313584Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT291010.12094928878651177Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT290870.12089110212478153Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG284650.11830595186791029Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG283910.11799839379876484Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA282240.11730431004812578No Hit
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT281400.11695519007774444Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTG280610.11662685105798104Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC277350.11527193307769162Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT276970.11511399785299531No Hit
AGCGACGCTCAGACAGGCGTAGATCGTCGGACTGTAGAACTCTGAACGTG272150.11311071421342626Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAG256780.1067226499934727Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT255320.10611584623542895Illumina RNA PCR Primer (100% over 38bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC253400.10531785773170023No Hit
TGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC249100.10353069597855771Illumina RNA PCR Primer (100% over 41bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTGGACTGTAGAAC248940.10346419693658031No Hit
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA246170.10231293227234667No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGATCGTCGGACTGTAGAACT245860.10218409037851546No Hit
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA244680.10169365994393217No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)