FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003057927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17815511
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG8262064.6375655461131595Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT5296932.9732125000512193Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT4609232.5872005579856787Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT3968992.2278283233077065Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC2972041.668231688667252Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2573411.4444772311049623Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT2402471.3485271345851377Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2216971.2444043844714865Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA2172931.2196843525846661Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1893141.0626358121302275Illumina DpnII expression Sequencing Primer (96% over 26bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC1820341.0217725441610965Illumina RNA PCR Primer (100% over 32bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT1683410.9449125540098176Illumina RNA PCR Primer (100% over 31bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG1506700.8457237067182637Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG1152550.6469362568382125Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT1126410.6322636493558899Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA1070720.6010043719767566No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC1069300.6002073137279083Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT1058500.5941451805676525Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG985110.5529507405092113Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG963060.5405738853070227Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT936010.525390486975086Illumina DpnII expression Sequencing Primer (96% over 30bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT906640.5089048526309462Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG881480.4947823276020542Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT872940.4899887519364446Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC834740.46854676242516985Illumina DpnII expression Sequencing Primer (100% over 26bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG788700.4427041132864502Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG777730.4365465576597831Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA774900.4349580542483457Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG773970.4344360372262126Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT734030.41201737070578554Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC707240.39697991261659576Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA702810.39449331540363897Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG689000.386741643279275Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC637990.3581092902695859Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT634530.35616716242380025Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT633260.35545430046884424Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC617220.3464509101086127Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC603270.33862065477661574Illumina RNA PCR Primer (100% over 39bp)
CCACGGGAACGTGGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA594690.33380462676596817Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGCTCAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT594420.33365307343696177Illumina RNA PCR Primer (100% over 31bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC560640.3146920680523842Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT539770.30297755702881607Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG531240.29818959444946597Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGTTTCAATTTCTATCGCCTATGATCGTCGGACTGTAGAACTCTGAACG521910.2929525849693562Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGACGCTCAGACAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT487220.2734807887351645Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT480750.2698491219252706Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG471510.26466263022149633Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG471030.2643932020810405Illumina DpnII expression Sequencing Primer (96% over 26bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG469930.26377576259249597Illumina RNA PCR Primer (100% over 33bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT454990.2553898117208089Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA446460.25060184914145883No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT441860.24801982946209064Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT424990.2385505529423209Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC407920.22896901469736122Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT391630.21982529718064223Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG386310.21683913529059032Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT371050.2082735656585994Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG367810.2064549257105227Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAACCCTTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG357650.20075203007087475Illumina RNA PCR Primer (100% over 34bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT349300.19606510304419558Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG343690.19291616165261832Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA339780.1907214449251554No Hit
AAGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAG337970.1897054763121866Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT334110.18753882501602115Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGACCCAGTAGCCAGATGTAGCTGATCGTCGGACTGTAGAACTCTGAAC326050.18301467749086736Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG325500.1827059577465951Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGGAGTGAAGACACGGAGCCAGAGATCGTCGGACTGTAGAACTCTGAACG323690.1816899891336263Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA323230.18143178716568947No Hit
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG320320.17979837906417617Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT319980.17960753413135327Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT317510.17822110182525777Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT312900.17563346905963012Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG301820.16941416948410853Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG298100.16732610139557602Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT297220.16683214980474037Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT294360.1652268071345245Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTG284270.15956320309869304Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA272250.15281627341477882No Hit
CAGGGGAAAGCGCGAACGCGATCGTCGGACTGTAGAACTCTGAACGTGTA266020.14931932067511283Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAGAGAGCTTGCCCTTGTATAGATCGTCGGACTGTAGAACTCTGAACGT262800.1475119068995551Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT254270.14272394432020502Illumina RNA PCR Primer (100% over 38bp)
AGCTGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGT252380.14166307101716027Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCAGCACCAGGATATTGTTGATCGTCGGACTGTAGAACTCTGAACGTG249760.14019244241717232Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAAGCCTACAGCACCCGGTATTCCGATCGTCGGACTGTAGAACTCTGAAC249030.13978268712022912Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGGTATTCTCGCACGGACTACAACCACGATCGTCGGACTGTAGAACTCTG246130.13815489210497528Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG245280.13767777977291812Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT244460.13721750669963945Illumina RNA PCR Primer (100% over 31bp)
TGGTGTCCTTGGAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC238870.13407979148058116Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT238610.13393385123783427Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT231030.1296791318531363Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACG230090.12915150174474369Illumina DpnII expression Sequencing Primer (96% over 26bp)
GTGAGCCCGTCTAAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT224650.12609798281957785Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT223430.12541318629591933Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT222300.12477890754859627Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG220270.12363945103791858No Hit
CCACGGGAACGTGGTGGATCTCGGACTGTAGAACTCTGAACGTGTAGATC219580.12325214808601337Illumina RNA PCR Primer (100% over 30bp)
AACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGTG218610.12270767871884224Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT218080.12241018514708897No Hit
AGCGACGCTCAGACAGGCGTAGATCGTCGGACTGTAGAACTCTGAACGTG217110.12186571577991784Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGTTCGTCCAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG215180.12078239013183512Illumina RNA PCR Primer (100% over 33bp)
AGACTCATTTGAGACGATGATGGATCGTCGGACTGTAGAACTCTGAACGT213570.11987868324405625Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAG211680.11881780994101152Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT209500.11759415713644139Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC207560.11650521840209915Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAAC205800.11551731522042787Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG204230.114636060677687Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG204080.11455186438379455Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT204050.11453502512501607Illumina RNA PCR Primer (100% over 38bp)
TGGTGTCCTTGGAAAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA203790.11438908488226916Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG200590.11259289727923044Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTG200530.11255921876167346Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTTAGCAGTTCTTGTGAGCTTTCTCGATCGTCGGACTGTAGAACTCTGA200270.11241327851892657Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA199760.11212701111969227No Hit
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT196630.11037011512047001Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCAGTTATCACAGTACTGTAGATCGTCGGACTGTAGAACTCTGAACGT188370.10573370587012633Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGTCTCCGTAGAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT186680.10478509429227148Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAACCCAGCAGACAATGTAGCTGATCGTCGGACTGTAGAACTCTGAACGT183750.10314046001823918Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCGGTCGAGGTCCGGTCGAGATCGTCGGACTGTAGAACTCTGAACGTG180820.10149582574420683Illumina DpnII expression Sequencing Primer (96% over 29bp)
CACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGT179340.10066508897780142Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)