FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003057929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28201454
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA26216909.29629373010342No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA11381914.0359302041660685No Hit
AGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGT4943491.7529202572321272Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT4229761.499837561566861Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA3638211.290078873238238No Hit
CAGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACG3515591.2465988455772528Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT3348241.1872579335803042Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG2993381.0614275419983665Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC2978171.0560342030591756No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA2504990.8882485278950512No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA2304040.8169933365847024No Hit
TATTCTCGCACGGACTACAACCACGACCAATGATCGTCGGACTGTAGAAC2154030.7638010437334188No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG2053110.7280156547956712No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA2001700.7097860982628769No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT1893520.6714263739734837Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA1788890.6343254500282148No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1759060.6237479812211101Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC1714760.6080395712930262Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA1679960.5956997820041476No Hit
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA1606720.5697294898341058No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA1603100.5684458680747454No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT1528340.5419365966024304Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT1483540.5260508908512306Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1453210.5152961262210097Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA1446550.5129345458571036No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA1430760.5073355437630982No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1268020.4496292992552795Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAATCAGCTAACTACACTGCCTGATCGTCGGACTGTAGAACTCTGAACG1266160.44896975879328777Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC1201540.4260560466137668No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1185770.4204641363526859Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG1182040.4191415095122401Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC1014740.35981832709760286No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA979630.3473686143983924No Hit
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA933750.33109994966926176Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT887460.3146859023651759No Hit
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA852530.30230001616228724No Hit
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC845450.2997895073069637Illumina RNA PCR Primer (100% over 32bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG752220.26673092812874116Illumina DpnII expression Sequencing Primer (96% over 29bp)
TACATCTGGACAGCCGAATGAAGATCGTCGGACTGTAGAACTCTGAACGT746540.2647168475781426Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC741780.26302899134207763Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC699900.24817869319787556Illumina RNA PCR Primer (100% over 39bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG691870.24533132227863144Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCTAGACCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG678750.2406790798800658Illumina RNA PCR Primer (100% over 37bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC677710.24031030456798433Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA673520.23882456557027168No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG665630.23602683748150005Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT656290.23271495150569188Illumina RNA PCR Primer (100% over 31bp)
AGGCGAACGTGATAACCACTACACTACGGAAGATCGTCGGACTGTAGAAC630830.22368704819262156No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA630830.22368704819262156No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT629840.22333600246285173Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT624620.22148503406951994No Hit
AGGCGAACGTGATAACCACTACACTACGGAAAGATCGTCGGACTGTAGAA607320.21535059858970393No Hit
GTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGAA594720.21088274384717895No Hit
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA575340.20401075774320004No Hit
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA569040.20177683037193755No Hit
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA565530.20053221369366273No Hit
GGTGTCAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG551420.19552892556532722Illumina RNA PCR Primer (100% over 36bp)
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA549330.19478782902470204No Hit
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG508820.180423321435838Illumina RNA PCR Primer (100% over 33bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT505160.1791255160106284Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAA499260.17703342529785876No Hit
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG482170.17097345406375147No Hit
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA461110.16350575399410258No Hit
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG433080.15356655015021564Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTTAGCAGTTCTTGTGAGCTTTCTCGATCGTCGGACTGTAGAACTCTGA419020.14858099160419175Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG409140.14507762613941821Illumina DpnII expression Sequencing Primer (96% over 29bp)
TATTCTCGCACGGACTACAACCACGACCAGATCGTCGGACTGTAGAACTC408550.14486841706814124Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG402970.1428897956821659No Hit
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG402350.14266994886150194Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG401560.14238982146097856Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA398540.1413189546893575Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG392890.1393155118881459Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG362320.12847564526282934Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC360150.1277061813905056No Hit
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG349690.12399715277091741Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA341620.12113559818582405No Hit
AGGTTTCAATTTCTATCGCCTATGATCGTCGGACTGTAGAACTCTGAACG341160.12097248602855726Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC340930.12089092994992386Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT336400.11928462979249226Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT331150.11742302364977351Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT330530.11720317682910959Illumina RNA PCR Primer (100% over 31bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC323980.11488060154628908Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG318950.1130970055657414Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC317650.11263603642563962Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT315910.11201904696119568Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT308760.10948371669063588Illumina RNA PCR Primer (100% over 38bp)
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG307960.10920004337365016No Hit
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG307940.10919295154072553Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT303840.10773912579117376Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG303320.10755473813513303Illumina RNA PCR Primer (100% over 40bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTGGACTGTAGAAC302310.10719660057243857No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCCGTCGGACTGTAG299780.10629948370747125No Hit
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG299570.10622501946176251Illumina DpnII expression Sequencing Primer (96% over 29bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT289980.10282448557439626Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT287490.10194155237527823Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCTGATGCCTTATCCATTAGGCCACTGGGTCGATCGTCGGACTGTAGA283590.1005586449549729No Hit
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA283360.10047708887633951No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)