FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003057930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16915208
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC267245515.799125851718761RNA PCR Primer, Index 6 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA4678572.76589563663657No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA3871772.2889284009986754RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC2719061.6074647145929273No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2212121.3077699074111298RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1856921.097781357462468RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1808571.0691976119950757No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC1323690.7825443234277699RNA PCR Primer, Index 6 (100% over 44bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1312710.7760531233195594RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC1253200.7408717646274288No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC1212630.7168874305299704RNA PCR Primer, Index 6 (100% over 36bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1107260.6545943744824184RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1103470.6523537871955225RNA PCR Primer, Index 1 (100% over 30bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1088230.643344143329482RNA PCR Primer, Index 6 (100% over 34bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1045130.6178641137608241No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA1023910.6053191896901298RNA PCR Primer, Index 6 (100% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA1019040.6024401237040655No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1000720.5916096331774342RNA PCR Primer, Index 1 (100% over 28bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTAT885770.5236530345946677RNA PCR Primer, Index 6 (100% over 48bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA872270.5156720508550648RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC721390.4264742118453406RNA PCR Primer, Index 5 (97% over 35bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA716850.4237902365729112No Hit
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC708850.4190607647272206RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC702340.4152121570127899No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA675030.3990669224995637RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG654780.38709544689015946RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC625630.3698624338524244RNA PCR Primer, Index 5 (97% over 35bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG596660.3527358339312174RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC589620.3485738987070097RNA PCR Primer, Index 1 (100% over 33bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT588250.34776397665343517RNA PCR Primer, Index 1 (100% over 30bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA574150.3394282825254055No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAAG561400.3318906867713362No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG485130.28680108456248365RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC425150.25134186939941855RNA PCR Primer, Index 1 (100% over 26bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG416950.24649416075758573RNA PCR Primer, Index 1 (100% over 29bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC415410.24558373742729028No Hit
GGGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC407100.2406709985475792RNA PCR Primer, Index 6 (100% over 44bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA407090.2406650867077721RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA386930.22874681765663185RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA382380.22605693054439532RNA PCR Primer, Index 1 (100% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG379750.22450211667512454RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC364010.21519688081872834No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC363680.21500179010509357RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC362250.2141563970126764RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT348440.20599214623905304RNA PCR Primer, Index 6 (100% over 43bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC345750.20440186133093957RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA342260.20233862923825707RNA PCR Primer, Index 1 (100% over 28bp)
AGCTGGTGTTGTGAATCAGGCCGTGGAATTCTCGGGTGCCAAGGAACTCC333980.19744362587796735RNA PCR Primer, Index 1 (100% over 27bp)
GCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT322970.19093469025033566RNA PCR Primer, Index 6 (100% over 46bp)
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG321910.19030803523078169RNA PCR Primer, Index 1 (100% over 29bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG316790.18728117324953972RNA PCR Primer, Index 6 (100% over 45bp)
GTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT313920.1855844752248982RNA PCR Primer, Index 6 (100% over 43bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC308510.18238616988924997RNA PCR Primer, Index 1 (100% over 33bp)
GGGGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT305870.18082544418017207RNA PCR Primer, Index 6 (100% over 43bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC301520.17825379386407783RNA PCR Primer, Index 6 (100% over 36bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC292250.17277351836288388RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG282170.16681438383731373RNA PCR Primer, Index 1 (100% over 29bp)
CCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTA277260.16391167049202116RNA PCR Primer, Index 6 (100% over 47bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCCCGCCAATATCTCGTATGC276950.16372840345800063RNA PCR Primer, Index 6 (98% over 50bp)
AATATAACACAGATGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG267730.15827768715584223RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA257810.15241314206718592No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC253190.14968187207629963RNA PCR Primer, Index 6 (100% over 50bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG247140.14610520899299612RNA PCR Primer, Index 1 (100% over 29bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA245330.14503516598790864RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC242410.14330890876423158RNA PCR Primer, Index 6 (100% over 44bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC242200.14318476012828218RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA241050.1425048985504642RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA238120.14077272948698RNA PCR Primer, Index 1 (100% over 28bp)
GGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC237800.1405835506131524RNA PCR Primer, Index 6 (100% over 44bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAA229900.13591319716553293RNA PCR Primer, Index 6 (100% over 38bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA218630.12925055370291633RNA PCR Primer, Index 1 (100% over 28bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA215930.12765435695499577RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA214460.12678531650335012No Hit
TATACAAGGGCAAGCTCTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA211800.125212767114658RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG210530.12446196345915461RNA PCR Primer, Index 1 (100% over 29bp)
GCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG209790.12402448731342824RNA PCR Primer, Index 6 (100% over 45bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC207940.1229307969491123RNA PCR Primer, Index 5 (97% over 35bp)
GCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT207820.12285985487142694RNA PCR Primer, Index 6 (100% over 43bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA207370.12259382208010684RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT204990.12118680420601391RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA201760.11927727994831634No Hit
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG184990.10936312459178747RNA PCR Primer, Index 6 (100% over 34bp)
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA184100.10883697084895438RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA183020.10819849214978615RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC182490.10788516464000915RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCC181480.10728806881949071RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT181200.10712253730489155RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC180640.1067914742756932No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA179090.10587513910559065RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA175520.10376461229445123RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATGGAATTCTCGGGTGCCA172260.10183735251733234No Hit
TGAGACCTCTGGGTTCTGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA171200.10121069749777832RNA PCR Primer, Index 1 (100% over 28bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC170530.10081460423070174RNA PCR Primer, Index 6 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)