FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003057931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16915208
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA248537414.693132948764212Illumina RNA PCR Primer (100% over 45bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA4283962.532608526008075No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT3650122.157892471674011Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA2565321.5165760893983686No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT1850221.0938204247917023Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG1814591.0727565395589578Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC1663280.9833044914375277No Hit
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC1307700.7730912915761958Illumina RNA PCR Primer (100% over 39bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1211860.7164322188648227Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT1169620.6914606075195765Illumina RNA PCR Primer (100% over 44bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA1125510.6653834821304No Hit
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT1083340.6404532536638036Illumina RNA PCR Primer (100% over 31bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT1060880.6271752614570273Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1020860.6035160785489602Illumina DpnII expression Sequencing Primer (96% over 30bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT984570.5820620118889464Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA933630.5519470999115116No Hit
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC929860.5497183363042298Illumina RNA PCR Primer (100% over 32bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA919240.5434399624290757No Hit
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG843910.49890607316209173Illumina RNA PCR Primer (100% over 43bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT788750.46629636478605524Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG687790.40661043009344017Illumina DpnII expression Sequencing Primer (96% over 26bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT671540.39700369040688116Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA670620.3964598011446268No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT656500.38811228333698294Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC652650.3858362250112443Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC621220.36725531249748744Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA611410.3614557976467094No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG577800.3415861040550019Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA571280.337731584500764Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG557770.32974468892135406Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT539590.3189969641520222Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT538150.3181456592197979No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA527830.3120446405388571No Hit
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG472090.279092045454008Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG402740.23809343639167782Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC400740.2369110684302552Illumina DpnII expression Sequencing Primer (100% over 26bp)
ATCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC393880.2328555463225755Illumina RNA PCR Primer (100% over 39bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA383760.22687276443777693No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT377480.22316012903890983Illumina DpnII expression Sequencing Primer (96% over 28bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT373910.22104960222777043Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG352950.20865838599206113Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT350640.20729275099661795Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGGATCTCGGACTGTAGAACTCTGAACGTGTAGATC346450.20481569011743753Illumina RNA PCR Primer (100% over 30bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG339570.20074834433014363Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT338630.20019263138827498Illumina RNA PCR Primer (100% over 38bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA329280.19466506116862412No Hit
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG327190.19342948664893747Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA326750.19316936569742446Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT312150.18453807957903917Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG311200.1839764547973634Illumina RNA PCR Primer (100% over 40bp)
TGGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT309840.18317244458359602Illumina RNA PCR Primer (100% over 38bp)
TGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC303450.17939477894685069Illumina RNA PCR Primer (100% over 41bp)
AGCGACGCTCAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT299820.17724878109686856Illumina RNA PCR Primer (100% over 31bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG297450.17584767506258273Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC292940.17318143530957467Illumina DpnII expression Sequencing Primer (100% over 26bp)
ATCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT283440.16756518749281712Illumina RNA PCR Primer (100% over 38bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG275990.1631608668365178Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAACGTGGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA265910.15720173231094764Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT260850.15421034136854833Illumina RNA PCR Primer (100% over 46bp)
TGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCC257800.15240723022737881Illumina RNA PCR Primer (100% over 42bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG254320.15034990997450343Illumina DpnII expression Sequencing Primer (95% over 24bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG248790.1470806625611698Illumina DpnII expression Sequencing Primer (96% over 29bp)
GATCGTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT247290.14619388659010282Illumina RNA PCR Primer (100% over 44bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG235390.1391587972196381Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA232980.1377340438261238No Hit
GCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC232840.1376512780688242Illumina RNA PCR Primer (100% over 39bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA231750.13700688752984888Illumina RNA PCR Primer (97% over 45bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT230660.13636249699087355Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGT229320.13557031045672036Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC229180.1354875446994208Illumina RNA PCR Primer (100% over 39bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG223420.13208232497052358Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT220890.13058662949932393Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG218620.12924464186310922Illumina RNA PCR Primer (100% over 33bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC210390.12437919770185504Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG208250.12311406398313281Illumina RNA PCR Primer (100% over 40bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT207870.12288941407046251Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT205970.12176616450711099Illumina RNA PCR Primer (100% over 38bp)
AGCGACGCTCAGACAGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAG205210.12131686468177039Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT204680.12100353717199339Illumina RNA PCR Primer (100% over 39bp)
ACAGAGAGCTTGCCCTTGTATAGATCGTCGGACTGTAGAACTCTGAACGT199600.11800032254997987Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC197870.1169775742633493No Hit
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG197200.11658148099627272Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC194740.11512716840372285Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGTCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGT190470.11260281280608551Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC183320.10837584734399955No Hit
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG176740.10448585675091905Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT174980.10344537294486712Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT172040.10170729204157583Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT171920.10163634996389048Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT171680.10149446580851977Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA170550.10082642791031597No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)