FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003057933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16841660
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT7704994.5749587629722965Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG6964994.135572146688628Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT4973672.953194637583231Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT3668042.177956329720467Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2930271.739893810942627Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT2781291.6514345973021662Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2072501.2305794084431105Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC2039361.2109020132219745Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1806151.0724299148658742Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT1533510.9105456350502266Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT1474570.8755490848289301Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG1446070.8586267624450321Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1337880.7943872516129645Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG1321920.7849107510779817Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1266940.7522655130195005Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT1241410.7371066747577139Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG1142620.6784485614838442Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGTGTCAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG1134900.6738646902977498Illumina RNA PCR Primer (100% over 36bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC1073910.637650920396208Illumina RNA PCR Primer (100% over 32bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT995260.5909512482736262Illumina RNA PCR Primer (100% over 31bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG911290.5410927426393836Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA892990.5302268303718279No Hit
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC883630.5246691834415372Illumina RNA PCR Primer (100% over 39bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG833900.4951412152958794Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC793720.47128370956307164Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG744500.4420585619232308Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT739420.4390422321790132Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT720460.4277844345509884Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC699500.41533910552760234Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG680190.40387348990538935Illumina DpnII expression Sequencing Primer (95% over 24bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT674400.4004355865158185Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG671110.3984820973704492Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCTAGCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC662210.3931975826610916Illumina RNA PCR Primer (100% over 32bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT634600.3768037117481293Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG622120.36939351584107505Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG615010.36517184173056577Illumina DpnII expression Sequencing Primer (96% over 29bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG569290.3380248740326072Illumina RNA PCR Primer (100% over 33bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT559060.3319506509453343Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC525040.3117507419102392Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC521790.30982100339277724Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT511330.3036102141950378Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC510920.3033667702589887Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG507110.3011045229508255Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT503770.2991213455205722Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG489760.2908026880960665Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA480970.2855834876134538Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTAGACCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG460820.273619108805189Illumina RNA PCR Primer (100% over 37bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG443320.263228209095778Illumina DpnII expression Sequencing Primer (96% over 26bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT426460.2532173194328825Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT413400.24546273942117344Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT413090.24527867205489245Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG412020.24464334275837418Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT397060.2357606079210719Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC387430.23004264425240742Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG374650.22245431863604895Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC372740.22132022615347896Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT372560.22121334832789644Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA370450.21996050270579026No Hit
AGGTTTCAATTTCTATCGCCTATGATCGTCGGACTGTAGAACTCTGAACG366870.21783482150809363Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG364390.21636228257784565Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT355020.21079869799057813Illumina RNA PCR Primer (100% over 38bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT353600.20995555069987162Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA352190.20911834106614194Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC349300.2074023581998449Illumina RNA PCR Primer (100% over 39bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT343820.20414852217655505Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG343260.2038160133858539Illumina DpnII expression Sequencing Primer (96% over 26bp)
GTCAGAGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG341950.20303818032189228Illumina RNA PCR Primer (100% over 37bp)
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT332070.1971717752288076Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAACCCTTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG318490.18910843705430463Illumina RNA PCR Primer (100% over 34bp)
AGCGACGCTCAGACAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT312680.18565865835078016Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT305200.18121729093212902Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG304230.1806413382053788Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT299660.1779278289669783Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT299010.1775418812634859Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG298620.17731031264139047Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG296910.17629497329835658Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAACCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG286850.17032169037968942Illumina RNA PCR Primer (100% over 36bp)
CAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT281330.16704410372849232Illumina RNA PCR Primer (100% over 31bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA280860.16676503385058242Illumina RNA PCR Primer (100% over 45bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT272900.16203865889704458Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT267640.15891545132724447Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT265590.15769823164699917Illumina RNA PCR Primer (100% over 31bp)
AACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGTG261570.15531129354232304Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT260870.1548956575539466Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT255640.15179026295507686Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGTGCCTTATCCATTAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG246480.1463513691643223Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT236600.14048496407123762Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT232660.13814552722237594Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT231550.13748644729795045Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGTCAGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG227790.13525388827467127Illumina RNA PCR Primer (100% over 37bp)
TCAGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTG225090.13365072089093355Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGT223640.13278976062929662Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA220040.13065220411764636No Hit
AGTCAGTTATCACAGTACTGTAGATCGTCGGACTGTAGAACTCTGAACGT214010.12707179696063214Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGTCAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT203050.12056412491405241Illumina RNA PCR Primer (100% over 38bp)
TATTCTCGCACGGACTACAACCACGACCAATGATCGTCGGACTGTAGAAC199470.11843844371635574No Hit
GGTCAGAGAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT199330.11835531651868048Illumina RNA PCR Primer (100% over 35bp)
AAGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACG194680.11559430602446552Illumina DpnII expression Sequencing Primer (96% over 26bp)
TACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGT194450.11545773991399896Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGGATCTCGGACTGTAGAACTCTGAACGTGTAGATC192260.1141573930360784Illumina RNA PCR Primer (100% over 30bp)
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT190520.11312424072211409Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT190370.113035175867462Illumina DpnII expression Sequencing Primer (96% over 28bp)
CACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGT190050.1128451708442042Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTG188560.11196045995466004Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT188050.11165763944884294Illumina RNA PCR Primer (100% over 38bp)
GGCTAGACCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG186430.1106957390186003Illumina RNA PCR Primer (100% over 36bp)
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC184310.10943695573951737Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG183600.10901538209416413Illumina DpnII expression Sequencing Primer (96% over 29bp)
TTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTG182990.10865318501857893Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGACCATGGCTGTAGACTGTTAGATCGTCGGACTGTAGAACTCTGAACGT181580.10781597538484923Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGAGAGCTTGCCCTTGTATAGATCGTCGGACTGTAGAACTCTGAACGT179860.10679469838483856Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGACTCATTTGAGACGATGATGGATCGTCGGACTGTAGAACTCTGAACGT179030.10630187285576363Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA178830.1061831197162275No Hit
ACAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG177890.10562497996040769Illumina RNA PCR Primer (100% over 40bp)
AGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTGTA175680.10431275776853352Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGGGGAAAGCGCGAACGCGATCGTCGGACTGTAGAACTCTGAACGTGTA175040.1039327477220179Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAATGATCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT173790.10319054059991711Illumina RNA PCR Primer (100% over 35bp)
AAGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAG170410.10118361254175658Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGAGTGAAGACACGGAGCCAGAGATCGTCGGACTGTAGAACTCTGAACG170380.10116579957082615Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGTTCGTCCAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG168950.10031671462314286Illumina RNA PCR Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)