FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003057935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20478965
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA217279510.609886778946104No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA11831695.777484360171522No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT4690902.2905942756384414Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA3324001.6231289032429128No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA3314701.618587658116511No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG3243331.5837372640658356No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT3081051.5044949781397645Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG2626641.2826038815926488Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC2308941.1274690884036376No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA1959860.9570112552074775No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA1926800.9408678612420109No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC1915580.9353890687346749No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA1873740.9149583487251431No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA1872600.9144016799677132No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA1581080.7720507359624863No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1511410.7380304619886796No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1451640.7088444166978165Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT1348840.6586465673436133No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT1341580.6551014663094546Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA1338740.6537146774751557No Hit
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA1229590.6004160854808825No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA1109290.541672882394203No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT1069380.5221845928248816Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC1065710.5203925100706994No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT980840.4789499859978275Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG936650.4573717470585061Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA933550.45585799868303895No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT923780.4510872497706793Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT923160.45078450009558585Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA859970.4199284485324331No Hit
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG813600.39728570267100904Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT808320.39470744737343905Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAG801340.391299071999No Hit
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA795760.38857432492315896No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG699150.34139908926061446No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA665490.3249627117386059No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG645140.315025686112555Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC634200.3096836192649384Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA633090.30914159968533567No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT630840.308042911348303No Hit
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG626060.3057088090145181Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAAC622960.30419506063905083No Hit
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC619440.3024762237740042No Hit
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA589930.28806631585141146No Hit
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA579350.2829000391377201No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG536350.2619028842522071Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC521610.25470525487982426Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG474990.2319404325365076Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA465660.22738453823227886No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG453710.22154928239781652Illumina DpnII expression Sequencing Primer (95% over 24bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT443930.21677365042618119Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA435150.21248632438211598Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG430340.21013757287050394Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC415080.20268602441578468Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT413930.20212447259907912Illumina RNA PCR Primer (100% over 31bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT412040.20120157439597167Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA408140.1992971812784484No Hit
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG407280.19887723818073816Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC401540.19607436215648594Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC395310.1930322162277244Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTGATCGTCGGACTGTAGAACT356910.1742812686090337No Hit
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT356900.17427638554975802Illumina DpnII expression Sequencing Primer (96% over 28bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT356440.17405176482307577Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC354060.172889596715459Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT352450.1721034241720712No Hit
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT340550.16629258363398736Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA340190.16611679350006212No Hit
GCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAACT323160.15780094355354385No Hit
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG319270.15590143349529628Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC317590.15508107953697856Illumina RNA PCR Primer (100% over 32bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG312760.15272256190681513Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT311900.15230261880910487Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG310480.15160922439195534Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC303210.14805924029852094Illumina RNA PCR Primer (100% over 39bp)
GCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACTC290490.14184798889982964Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG288420.1408371956297596No Hit
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG282200.13779993276027377Illumina RNA PCR Primer (100% over 33bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG281690.13755089673721305Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGGA279360.13641314392597476No Hit
AACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGT276050.13479685130571786Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCCGTCGGACTGTAG262930.12839027753599852No Hit
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG262860.1283560961210686Illumina DpnII expression Sequencing Primer (96% over 26bp)
GTCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGT261510.12769688311884902No Hit
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA257780.12587550200901268No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTGGACTGTAGAAC253100.12359023026798474No Hit
CAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGA250080.12211554636672312No Hit
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA250040.12209601412962032Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT248420.12130495852695682Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG245690.11997188334469053Illumina RNA PCR Primer (100% over 40bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT242060.11819933282761116Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT241080.1177207930185925Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT240450.11741316028422336Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT233620.11407803079891976Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA231270.11293051186913011No Hit
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG229160.11190018636195727Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG228730.11169021481310212Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCTGATGCCTTATCCATTAGGCCACTGGGTCGATCGTCGGACTGTAGA227720.11119702582625636No Hit
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA226250.11047921611272835No Hit
GCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG223170.10897523385581254Illumina RNA PCR Primer (100% over 40bp)
AAACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACG221260.1080425695341537Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG218540.10671437741116312No Hit
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA218410.106650897640579No Hit
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT216550.10574264861529868Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT215350.1051566815022146Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT212940.10397986421677072Illumina RNA PCR Primer (100% over 38bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT211210.1031350949620745Illumina DpnII expression Sequencing Primer (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)