FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003057937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17225104
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGT6211263.6059346869545754Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT4772432.770624781133397Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT4746362.755489894284528Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACG4351522.526266314560423Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG4083982.3709464976234687Illumina DpnII expression Sequencing Primer (96% over 26bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT2754261.5989801861283393Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA2710751.5737205418324325No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT2663391.54622578766433Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2277011.3219136441788684Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1590920.923605453993195Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA1446600.8398207639268825No Hit
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC1435190.8331967110329204Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT1108680.6436419774301508Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1009150.5858600331237478Illumina DpnII expression Sequencing Primer (96% over 30bp)
TACATCTGGACAGCCGAATGAAGATCGTCGGACTGTAGAACTCTGAACGT1004070.5829108491884868Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG992640.5762751853341495Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA880160.5109751441849059Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT844310.49016249771264075Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA836520.4856400286465615No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG794860.46145439818534617Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG677170.3931297018584039Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAATCAGCTAACTACACTGCCTGATCGTCGGACTGTAGAACTCTGAACG665050.38609345987112764Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT662260.3844737308988091Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGTGTCAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG623460.3619484677712251Illumina RNA PCR Primer (100% over 36bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT613620.35623587526670375Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG560090.32515913982290034Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG557810.3238354903401454Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT557560.32369035333545737Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG553190.3211533584935104Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC519940.3018501368700009No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC513390.2980475473471742Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG511830.2971418924379209Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG503750.29245106444640334Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC485790.2820244220296145Illumina RNA PCR Primer (100% over 39bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT464000.2693742807010048Illumina RNA PCR Primer (100% over 31bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG451380.2620477647043524Illumina DpnII expression Sequencing Primer (96% over 26bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG432510.25109282359049906Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG420930.24437007753334902Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT419080.2432960636986575Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT417790.2425471567544672Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA417290.2422568827450911No Hit
GCTAGACCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG411860.23910450700326685Illumina RNA PCR Primer (100% over 37bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC409120.23751380543188594Illumina RNA PCR Primer (100% over 32bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG407180.2363875422755067Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG406330.2358940764595674No Hit
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG365960.21245735294254248Illumina DpnII expression Sequencing Primer (96% over 29bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG361410.20981585945722012Illumina RNA PCR Primer (100% over 33bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA359140.20849801545465269Illumina DpnII expression Sequencing Primer (96% over 30bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT358350.20803938251983847Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG358290.20800454963871337Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG338730.196649030391921Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC333690.19372306837741007Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG333220.1934502108085966Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA329140.19108157489208774No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA320960.18633269209869502No Hit
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA305940.1776128608570375No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT295980.17183060259026592No Hit
TATTCTCGCACGGACTACAACCACGACCAATGATCGTCGGACTGTAGAAC291690.16934005158981913No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC291400.169171692664381Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT282040.16373776320886074Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG261910.15205133159137965Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAACTCTGAACGT261070.15156367125562784Illumina DpnII expression Sequencing Primer (96% over 28bp)
GACCCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG251350.1459207445133568Illumina RNA PCR Primer (100% over 36bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT249600.1449047854805405Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT246020.14282642357340775Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGACGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG241140.13999334924189719Illumina RNA PCR Primer (100% over 36bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG239950.1393024970995821No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT238350.13837362026957864Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG233300.1354418527748802Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC232370.1349019431174407Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC231230.13424011837606323Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGTG229250.13309063329893392Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT222190.12899196428654364Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA220840.1282082244612282No Hit
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT220130.12779603536791417Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT220040.12774378604622647Illumina RNA PCR Primer (100% over 38bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT208170.12085268106363828Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC207150.12026052208451107Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGCTAGCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC201900.1172126449860622Illumina RNA PCR Primer (100% over 32bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT194590.11296883896898388Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT193690.11244634575210692Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT191740.1113142771155402Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAACCCTTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG188310.10932299741122029Illumina RNA PCR Primer (100% over 34bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG184520.10712272042014957Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT183960.10679761352964837Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAAC183610.1065944217230851Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG182450.10592098602133258Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT181490.10536365992333051Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGTG180600.10484697218664109Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA179730.10434189541032671No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGATCGTCGGACTGTAGAACTCTGA179310.10409806524245079Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGACTAGTCAAGTGCAGTAGTGAGAAGGATCGTCGGACTGTAGAACTCTG178820.10381359671326222Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA177210.10287891440307123No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA176670.10256541847294506No Hit
TGGGCCCGATAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG174980.1015842923212539Illumina RNA PCR Primer (100% over 34bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG173970.10099793882231423Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC172920.10038836340262444Illumina DpnII expression Sequencing Primer (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)