FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003057939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23094797
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA240491310.413224242672495No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA22160959.595646153547053No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT7790613.373318241333752Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG7108603.078009302268385Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC6064782.6260373711013782No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA4721462.0443825507537476No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT4716542.0422522007879094Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA4613031.997432581892796No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA3996141.730320470017554No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA3821061.6545111870868576No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA3151961.3647922516920152No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG2938661.2724337867096214No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2688611.164162646677518Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA2446761.0594420899218122No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA2416041.046140392574137No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT2237900.9690061358841994Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2118500.9173061793961644Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA1993610.8632290641047851No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA1665820.7212966626205894No Hit
TGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA1618320.7007292594951148No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA1605600.695221525437093No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC1502580.6506140755426427No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA1278000.5533713935653992No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT1150690.49824642320952206No Hit
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA1078060.4667977813357701No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1071340.4638880350409662Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC928240.40192602688822077No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA882740.3822246196838188No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG873840.3783709378350457Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG858810.371862978488185Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC845310.3660175060209449No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG831680.36011574381883504Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA772400.3344476247182428No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT766250.3317846872609445No Hit
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG737790.31946156530408126No Hit
GCAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAG704300.3049604636057204No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT690920.29916695089374457Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA678990.2940012852245465No Hit
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA676610.29297075007847007No Hit
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA665280.2880648831855937No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC654120.28323262594600856Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA635450.2751485540227957No Hit
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG596950.2584781325421479Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTGGACTGTAGAAC581000.2515718150715938No Hit
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA545270.23610079794163163No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG524820.22724598964866416Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC470790.20385110984088756Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG467930.2026127356737537Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG457760.19820914641509946Illumina DpnII expression Sequencing Primer (95% over 24bp)
CGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA440000.19051910263597469No Hit
TGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC439670.1903762133089977No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC432690.18735388754445428Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT401020.17364084213426947Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA399470.17296969529543818No Hit
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT395820.17138925273948066No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA379180.16418416667615654No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG375420.16255609434454002No Hit
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG364630.15788404635035327Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC364260.15772383710495483Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT353140.15290889978379113Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT352900.15280498027326242Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACATGCGATCGTCGGACTGTAGAACT351500.1521987831285116No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT351070.15201259400548098Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG344600.14921109720081108Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA335480.14526215580071997No Hit
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT327330.1417332224223491Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC321220.13908760488347224Illumina DpnII expression Sequencing Primer (96% over 30bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT320150.13862429706569837Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT314720.13627311813998624Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC313600.13578816042418557Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAAC310980.13465370576758046No Hit
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT310690.13452813635902494Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG308340.1335105911517646Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA307360.13308625315043904No Hit
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG306390.1326662451287188Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA306090.13253634574055792Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT298070.12906370209705675Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA290650.12585085722987738No Hit
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG285860.12377679699890845Illumina DpnII expression Sequencing Primer (96% over 26bp)
CCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG281460.1218716059725487Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT279640.12108354968437263Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCCGTCGGACTGTAG275690.11937320774025421No Hit
AGGCTGATGCCTTATCCATTAGGCCACTGGGTCGATCGTCGGACTGTAGA275040.11909175906590563No Hit
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG264880.11469249978685676Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG263630.11415125233618638Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG263070.11390877347828604Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT261870.11338917592564249Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT259520.11237163071838215Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG251560.10892496695251315Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAAC247810.10730122460050201No Hit
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT246430.10670368741496192Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG244970.10607151039257891Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAAACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTA237760.10294959509711213No Hit
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT234290.10144709217405115Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCAGCACGCTTCCGCTGCGCACTCTGCTGATCGTCGGACTGTAGAACT233650.10116997347930791No Hit
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG231800.10036892725231576Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA231260.10013510835362614No Hit
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG231010.10002685886349207Illumina DpnII expression Sequencing Primer (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)