FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003057941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16825247
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT7853804.667866094328363Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG6789314.035191875637843Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT5897933.5054047052028423Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT4071442.4198396611948696Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT2588431.538420208630518Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2542121.5108960956115534Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1939641.152815171153208Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1874931.1143551116961314Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT1647380.9791119262617659Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA1619630.9626188548673312No Hit
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG1545780.9187264828861057Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG1218490.7242033356181933Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1088180.646754249729588No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG1062970.6317708144195446Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC972610.5780658078897741Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG947670.5632428457068119Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG877030.5212583209031048Illumina DpnII expression Sequencing Primer (95% over 24bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA870850.5175852693276954Illumina DpnII expression Sequencing Primer (96% over 30bp)
TATTCTCGCACGGACTACAACCACGACCAATGATCGTCGGACTGTAGAAC833520.49539837364646117No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA806760.47949370371799No Hit
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT800350.4756839528121043Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC777430.46206156735767384Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA755730.44916428269968345Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG746140.4434645149637328Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT744600.44254922379445605Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT736360.4376518216939103Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC716000.42555095922217373Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC686510.40802372767543915Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG673460.4002675265331914No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT665260.3953938982292504Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG647170.3846421987148242Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT641700.38139113202914643Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA637660.3789899785720828No Hit
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT614000.3649277778804674Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG575790.34221785867393206Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT552320.3282685835161885Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG527080.3132673178586918Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG513180.3050059235385965Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC497090.2954429138543999Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA495270.294361206108891No Hit
GTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGAA482000.286474249085318No Hit
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG471200.28005532400207855Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG462570.2749261273846381Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG455480.2707122219364744Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT454670.2702308025552314Illumina RNA PCR Primer (100% over 31bp)
AATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGT452900.2691788120554783No Hit
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG448710.2666885068611474Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT444850.2643943354888044Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA430000.2555683134993501No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC421580.2505639293140838Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT409340.243289147553079Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG408840.24299197509552165No Hit
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG400280.2379043826221392No Hit
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG399980.2377260791476048Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG386020.22942902413260266Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG385640.22920317306485902Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC377570.22440680959988282Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA369550.21964016338066242No Hit
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT366250.21767882516078368Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG364490.2166327781101817Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT362820.21564022210194003Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA349820.20791373820544806No Hit
GGTGTCAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG322180.19148604475167585Illumina RNA PCR Primer (100% over 36bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG314280.1867907199222692Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT313850.18653515160876985Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC313170.1861309970664918Illumina RNA PCR Primer (100% over 32bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC303280.18025292585600675No Hit
AAGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACG303190.18019943481364642Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT299110.17777450755997817Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG298950.1776794123735598No Hit
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA296450.1761935500857729Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC296320.17611628524680795Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT296270.1760865680010522Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT293040.17416683392523152Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG283040.16822338477408386Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT281670.16740913224037662Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT276770.16449684215631427Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT272730.1620956886992506Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT268000.15928443725075775Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG266900.1586306578441315Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTGATCGTCGGACTGTAGAACT261210.15524883527712846No Hit
GAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT260760.15498138006532683Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG254160.15105870362556936No Hit
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT252740.1502147338461064Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT252230.14991161793939786Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGA245170.1457155428386876No Hit
AGGTTTCAATTTCTATCGCCTATGATCGTCGGACTGTAGAACTCTGAACG244260.14517468896593316Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT238210.14157890222948882Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA237220.1409905007635252No Hit
GAGACCCAGTAGCCAGATGTAGCTGATCGTCGGACTGTAGAACTCTGAAC232310.13807226723031168Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGTG229800.13658046149337363Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGAGAGCTTGCCCTTGTATAGATCGTCGGACTGTAGAACTCTGAACGT228790.1359801731291077Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC227910.13545714960380673Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGT222520.13225363051133812Illumina DpnII expression Sequencing Primer (96% over 28bp)
TATTCTCGCACGGACTACAACCACGACCAGATCGTCGGACTGTAGAACTC218960.13013776261352952Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA213180.1267024490041662No Hit
AGACTCATTTGAGACGATGATGGATCGTCGGACTGTAGAACTCTGAACGT210610.1251749825723212Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC208010.12362968579302284No Hit
CAATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAGA205920.12238750492043297No Hit
CAACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAG205320.1220308979713641No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA204340.12144843995455162No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT197920.11763274559951484Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCT197590.11743661177752696Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG197560.1174187814300735Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG194250.11545149976104363Illumina DpnII expression Sequencing Primer (96% over 26bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG194000.11530291353226495Illumina RNA PCR Primer (100% over 33bp)
TCAATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAG191550.11384676849023376No Hit
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT188350.11194486476186649Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGTG188320.11192703441441307Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT186930.11110089498240353Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATAGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAAC186390.11077994872824157Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT183980.10934757748281496Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT183500.10906229192355987Illumina RNA PCR Primer (100% over 31bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT183380.1089909705337461Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGA183280.10893153604223463No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA182780.10863436358467723No Hit
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA182470.10845011666099166No Hit
CCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG181330.10777256345776082Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCAGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTG180970.10755859928831951Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGTGTTTATGGGGTGATGTGAGCCCGTCTAAGATCGTCGGACTGTAGAAC179600.10674434675461228No Hit
TACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGT179500.10668491226310081Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGGCCTCGATCAGAAGGACTTGGGCCCCCCAGATCGTCGGACTGTAGAAC173200.10294053929787778No Hit
AAGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT172720.10265525373862266Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGAGTGAAGACACGGAGCCAGAGATCGTCGGACTGTAGAACTCTGAACG172530.10254232820475087Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGACCATGGCTGTAGACTGTTAGATCGTCGGACTGTAGAACTCTGAACGT171920.10217977780653087Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGACTAGTCAAGTGCAGTAGTGAGAAGGATCGTCGGACTGTAGAACTCTG171250.10178156671340396Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCGACGCTCAGACAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT170450.10130609078131217Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)