FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003057943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16274110
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT7345734.513752211334444Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT6733184.1373568201271835Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA6685124.107825251273342No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG5679373.4898191053151293Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT3314612.03673810733736Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT2893841.7781863339992172Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG2651031.628986162684165Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2315201.4226277197339825Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA2116841.300740870007638No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT1809321.1117781556103528Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1670831.0266798000013517Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG1636841.0057938652251952Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG1618340.9944261160825385Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG1294980.7957301505274328Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC1094580.672589776030763Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG1076330.6613756451197639Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG999900.6144114793374261Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC990650.6087276047660979Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT985390.6054954771720235Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC888040.5456765377645844Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT887540.5453693013012694Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT839110.5156103774645742Illumina DpnII expression Sequencing Primer (96% over 28bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT819780.5037326157928145Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGTGCCTTATCCATTAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG777520.47776498991342686Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG763120.4689165797699536Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT745180.4578929354662098Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT714660.4391392217454595Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT711890.43743713173869414Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT685750.4213748094365836Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA683140.41977103509807906Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG657520.4040282387178162Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA642880.3950323550719517No Hit
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG635740.3906450183758129Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA622160.38230047603217626No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC609870.37474860376389246Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG608780.3740788282738657Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG586700.3605112660538733Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT581920.35757408546458147Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG572970.35207455277124217Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA565020.34718949300453295No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA539030.3312193416414169No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC528560.3247858100995999No Hit
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC520200.3196488164329724Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT500190.3073532131711043Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT483800.2972820019036371Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG475680.2922924817394008Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG459040.2820676522402761Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA439480.27004856179539155Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT430230.26436468722406326Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA423380.26015554767664717No Hit
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC419110.25753174827993663Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG401510.2467170247712471Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA400300.2459735125300247No Hit
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT388450.23869200834945814Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT384120.23603134057714986Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG382280.2349007103921505Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT379800.23337681753410783Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT377620.23203726655405427Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC373590.229560940659735Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA362160.2225375151083531No Hit
TCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGTG359840.22111193791857128Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG354570.2178736655952307No Hit
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC346510.21292101380659217Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT346030.21262606680180976Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG344030.2113971209485496Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT340360.20914200530781712Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTGATCGTCGGACTGTAGAACT338590.20805438822768188No Hit
AAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT334860.20576240421135164Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA334110.20530154951637908No Hit
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG329840.20267775011966863Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGACCCAGTAGCCAGATGTAGCTGATCGTCGGACTGTAGAACTCTGAAC329520.20248111878314695Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG329190.20227834271735906Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT313350.19254509155953842Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACG312070.19175856621345191Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC311100.19116252747462073No Hit
AACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGT308720.18970008190924112Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA298280.183284984555223No Hit
AACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGTG289510.1778960569886771Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGT288990.17757653106682947Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT286860.17626770373310738Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA286530.17606492766731943No Hit
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT278980.17142565707126228Illumina RNA PCR Primer (100% over 31bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT268920.16524405942936357Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGAGAGCTTGCCCTTGTATAGATCGTCGGACTGTAGAACTCTGAACGT260580.16011935522126863Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT254680.1564939649541511Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGACTCATTTGAGACGATGATGGATCGTCGGACTGTAGAACTCTGAACGT254020.15608841282257524Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT251490.15453379631820113Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT250730.15406679689396224Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACG244580.15028778839518722Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG239620.14724000267910195Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG238740.1466992665036675Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG237020.14564237306986375No Hit
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA226980.13947306488649763No Hit
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG226710.1393071571963075Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGCCTATGGAATTCAGTTCTCAGATCGTCGGACTGTAGAACTCTGAAC225090.13831171105516676Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA220310.13537453046587494No Hit
GGGAGTGAAGACACGGAGCCAGAGATCGTCGGACTGTAGAACTCTGAACG216620.1331071253666099Illumina DpnII expression Sequencing Primer (96% over 26bp)
ATAGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAAC216060.13276302052769706Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG214770.13197035045234426Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA214600.13186589005481714Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT213630.13126985131598595No Hit
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG213600.13125141712818705Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA209180.12853544679248205No Hit
AAACCCAGCAGACAATGTAGCTGATCGTCGGACTGTAGAACTCTGAACGT206900.12713444851976544Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGT206410.1268333567857167Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTG203570.12508825367408724Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCGGTCGAGGTCCGGTCGAGATCGTCGGACTGTAGAACTCTGAACGTG198690.1220896257921324Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC197850.12157346853376312No Hit
AAACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACG197120.12112490329732317Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGGCCTCGATCAGAAGGACTTGGGCCCCCCAGATCGTCGGACTGTAGAAC196670.12084839048033963No Hit
TGGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTG195970.12041825943169857Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT195620.12020319390737805Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT188290.11569910735517949Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT187990.11551476547719045No Hit
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT178900.10992920657412295Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGTACGCTATACGGTCTACTAGATCGTCGGACTGTAGAACTCTGAACGTG178480.10967112794493832Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGTACGCTATACGGTCTACTATGATCGTCGGACTGTAGAACTCTGAACGT174050.10694901287996701Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG173390.10654346074839116Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC173060.10634068468260323No Hit
AACTATGCAACCTACTACCTCTGATCGTCGGACTGTAGAACTCTGAACGT168220.1033666357177136Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACG168070.10327446477871909Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACG165380.10162153260608416Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGACCATGGCTGTAGACTGTTAGATCGTCGGACTGTAGAACTCTGAACGT162890.10009149501877522Illumina DpnII expression Sequencing Primer (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)