FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003057945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19652208
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA7229563.6787520262354234No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA6895793.508913604008262No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT6174853.142064240313353Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG4945012.5162617859530085Illumina DpnII expression Sequencing Primer (96% over 26bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT3736821.9014759054046242Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT3711881.8887852194521857Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG3376581.718168258752401No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT3071871.5631169790183375Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC3013451.533390039429666No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA2716781.382429903041938No Hit
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA2615311.3307970279980752No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2103351.0702868603873925Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT1994351.0148223548214022Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC1582150.8050749310204737No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT1385380.7049487772569881Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1376320.700338608262237Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT1322830.6731202926409083Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG1300920.6619714181734694No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1236480.6291812095617958Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC1200070.6106540293080553No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG1079560.5493326754937664Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA1053950.5363010609291332No Hit
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG1034260.5262818305200108Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG903790.45989234390354505No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG895490.45566889990173115Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA820950.4177393196733924No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA817380.4159227299039375No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG753660.38349889233820444Illumina DpnII expression Sequencing Primer (96% over 26bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA702590.3575119905101758Illumina RNA PCR Primer (100% over 45bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG701230.3568199563122882Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC696240.3542808014244506Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG691470.3518535932450949Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA625460.3182644922138011No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC606490.30861163285061916Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG591020.30073974384964786Illumina DpnII expression Sequencing Primer (96% over 29bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT556740.2832964112734813Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA540080.2748189923493584Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG532560.27099245031398No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG527350.26834134871766063Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT517200.2631765346672496Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA506270.25761481865040303No Hit
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC489880.24927478886850782Illumina RNA PCR Primer (100% over 39bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG489850.2492595234082603Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT486500.24755488034728718Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCGACGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT483470.24601306886228763Illumina RNA PCR Primer (100% over 35bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT458570.2333427368568458No Hit
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA458520.23331729442309995No Hit
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG450280.229124381341781Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT449830.22889539943806822Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT445420.22665137678168273No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT438810.2232878870404791Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG431180.21940537165085977Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC420320.21387927504125745Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT414470.21090251029299098Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT400910.20400252226111185Illumina RNA PCR Primer (100% over 31bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG396750.20188571177345566Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG393870.20042022758969374Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT391000.198959831892681Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG386690.1967666941037872Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA385350.19608483687939796No Hit
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA385110.19596271319741784No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT384440.19562178458522322Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC380390.19356094745180796Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG368810.18766847979626514Illumina RNA PCR Primer (100% over 40bp)
GGCGACGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG362740.18457976833951686Illumina RNA PCR Primer (100% over 36bp)
CAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAA352360.17929791909387485No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG346880.17650942835532782Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG343370.17472336950636794Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC337470.1717211623243556Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGTGTCAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG329880.167859000881733Illumina RNA PCR Primer (100% over 36bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT326190.165981349271288Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT324780.16526387263965456Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT318060.16184440954420998Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA301120.1532245129911102No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA292740.1489603610953029No Hit
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA283680.14435019210055175Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT281660.1433223177772187Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT280370.14266590298657536Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCTAGCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC280030.14249289443710345Illumina RNA PCR Primer (100% over 32bp)
ACAAACCCTTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG274320.13958736850332543Illumina RNA PCR Primer (100% over 34bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA274230.13954157212258286No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA273900.13937365205986013No Hit
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT272930.13888006884519033Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG272210.13851369779924982Illumina RNA PCR Primer (100% over 33bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT266720.13572011857395364Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCCCGTACGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG264820.13475330609161068Illumina RNA PCR Primer (100% over 34bp)
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG256260.13039756143431822Illumina RNA PCR Primer (100% over 40bp)
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG256230.13038229597407072Illumina RNA PCR Primer (100% over 43bp)
GGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT256130.13033141110657898Illumina RNA PCR Primer (100% over 38bp)
TGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA252610.1285402637708699No Hit
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG249330.12687124011714102Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGTG247700.12604181677702578Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTGGACTGTAGAAC247320.1258484542805572No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTGGACTGTAGAAC246360.1253599595526365No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA243430.12386903293512871No Hit
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT240880.1225714688140895Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT236080.12012899517448625Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGT235930.12005266787324864Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG231170.1176305481806421Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC228200.1161192676161376No Hit
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG227630.11582922387143468Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCAGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTG219220.11154980651537985Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG214450.10912259833602413Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC210450.10708720363635477Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC209720.1067157441036651Illumina RNA PCR Primer (100% over 32bp)
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG202710.10314871489249453No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC200900.10222769879089415Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAGAATTCTACCACTGAACCACATGCGATCGTCGGACTGTAGAACT197260.10037548961419501No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)