FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003057947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18064990
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT7565104.187713361590569Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA6112803.383782664701171No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT6039913.3434339017071144Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG5293712.930369737265285Illumina DpnII expression Sequencing Primer (96% over 26bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT3486441.9299429448895349Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2652711.4684259443265675Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT2454771.358854890038688Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA2193751.2143654660201861No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG2083441.1533026035442036Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1842151.0197348573124039Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1834301.0153894355878414No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT1717060.9504904237422772Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG1382490.7652868891707109Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG1245320.6893554881569268Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG1145110.6338835504475784Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG1060310.5869419246841543Illumina DpnII expression Sequencing Primer (95% over 24bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG1056420.5847885883136387Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG971600.5378358914120628Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG901180.49885441397974756No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC721680.39949094906778254Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT718270.397603320012909Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT707790.3918020436213914Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG671320.3716138232016735Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT662360.3666539533096891Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA660450.36559665961619686No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC658900.36473864640943615Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC647920.3586605915641249Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT622140.34438989448651786Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC614600.3402160754033077No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT606320.33563262420848283Illumina DpnII expression Sequencing Primer (96% over 28bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT589520.326332868161012Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG557230.30845851561501003Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA554500.306947305257296Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC550100.3045116548639108Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG529980.2933740898832493Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG528870.29275964171582713Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG513820.2844286102566345Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA513650.28433450558234463No Hit
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC490420.2714753786190859Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT474850.2628564975679477Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG473160.26192098639412476Illumina DpnII expression Sequencing Primer (96% over 29bp)
TATTCTCGCACGGACTACAACCACGACCAATGATCGTCGGACTGTAGAAC449630.24889579235858975No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA448770.24841973341806445No Hit
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG447000.2474399376916345Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT444450.24602836757728624Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC436670.2417216948362551No Hit
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG433980.24023262675484458Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG430340.23821767961122592No Hit
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT419680.23231676297634263Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGATCGTCGGACTGTAGAACTCTGA415780.23015789103675122Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC400210.22153900998561304Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG398770.22074188803868697Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG385380.2133297610460897Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT375460.20783847652282122Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA373920.20698599888513639Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT373040.20649886880645935Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG356580.19738732210756832Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG353190.1955107641908465Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT351760.1947191778129963Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA343220.18999180182219863No Hit
AGACTAGTCAAGTGCAGTAGTGAGAAGGATCGTCGGACTGTAGAACTCTG334430.18512603660450405Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA330780.18310555389180952No Hit
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG321970.1782287175359632Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT319420.17681714742161497Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA304910.16878503669251962No Hit
GAGCTAGCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC304290.16844183140981533Illumina RNA PCR Primer (100% over 32bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA301840.16708561698622584No Hit
CAGTGCCTTATCCATTAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG301760.16704133243361885Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACG295560.16360927960657604Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC295460.16355392391581727Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT291210.1612013070585702Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC289330.1601606200723056Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT283300.15682267191955268Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA282500.15637982639348263No Hit
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT282480.1563687552553309Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT280030.1550125408317414Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA274590.15200119125446512No Hit
AAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT269420.14913930204223752Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT265460.1469472166881908Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT261550.1447828091795235Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGTG260170.14401890064705267Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT257500.1425409037037939Illumina RNA PCR Primer (100% over 31bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA251260.1390867086004476No Hit
GCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGTG250590.13871582547236394Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA241850.13387773810004877No Hit
AAAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCT237040.13121512937455265Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA235030.1301024799903017No Hit
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG234840.12999730417786004Illumina DpnII expression Sequencing Primer (96% over 26bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT226830.1255633133480838Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT223320.12362032860245148Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCAGTCAAATTTAGCAGTGGGGGATCGTCGGACTGTAGAACTCTGAA212230.11748138249730557Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGGCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGT210660.1166122981523931No Hit
AGCTGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGT207970.1151232300709826Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACGTG207900.11508448108745147Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA207000.11458627987062268No Hit
CAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT201280.11141993435922189Illumina RNA PCR Primer (100% over 31bp)
AGACTCATTTGAGACGATGATGGATCGTCGGACTGTAGAACTCTGAACGT199640.11051210103077833Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG198950.11013014676454291Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAGACTAGTCAAGTGCAGTAGTGAGAAGGATCGTCGGACTGTAGAACTCT195280.1080985929136966Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT194080.10743432462459156Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG191310.10590097199057404Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG191240.1058622230070429No Hit
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT189260.10476618033001955Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT188200.10417941000797676Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT186790.10339889476827832No Hit
ATAGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAAC186690.10334353907751954Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG182490.10101860006565185Illumina DpnII expression Sequencing Primer (96% over 26bp)
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG182420.10097985108212072No Hit
CAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTGAACG182110.10080824844076858Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAA181930.10070860819740282No Hit
AAGCCAGTCAAATTTAGCAGTGGGGATCGTCGGACTGTAGAACTCTGAAC181850.10066432364479583Illumina DpnII expression Sequencing Primer (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)