FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003057987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18744229
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG9542645.090974934205082Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT8867654.730869431866203Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA7650604.081576254750195No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT6204583.3101281466418273Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT6082053.244758693462398Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT3644441.9442997628763499Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT3610461.9261715165771822Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA3050491.6274289009166503No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2100721.1207289454263498Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT1809560.9653958026227699Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG1696600.9051319208701515Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1695990.904806487372727Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG1535660.8192708273036997Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG1388320.740665300237209Illumina DpnII expression Sequencing Primer (96% over 26bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT1350700.7205951229042283Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT1308020.6978254480352326Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG1222150.6520140145534927Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT1061850.5664943594105685Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG1034850.5520899259179985Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG1017710.5429457781379005Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG926720.49440283726793993Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC905200.48292197027682493No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG827350.4413891870399151Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGTG808810.4314981427083504Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC797740.4255923249763967Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA791360.4221886106918561No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT783020.41773924123526235Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT782730.4175845269496014Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG778450.41530115749226065Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA761140.40606631513091307No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT755790.40321210330923724Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT746420.3982132313897787Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG712980.3803730737604625Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGT706460.37689466982077524Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC665460.35502127081353946Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG654280.34905676835254196Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA651600.3476269949540203No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA616850.329087955551546No Hit
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC613950.3275408126949367Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA599410.3197837585104194No Hit
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT553750.2954242609818734Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC551440.29419188167195354Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA542810.2895877979296988No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT526830.28106250729224447Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG519490.27714663537241246Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT508890.2714915614827369Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT505070.26945360089230663Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG499040.2662366107456327Illumina DpnII expression Sequencing Primer (96% over 26bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT486240.25940784227508107Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG477160.2545636846412835Illumina DpnII expression Sequencing Primer (96% over 29bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG473070.2523816797159275Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC469890.2506851575490248No Hit
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT467870.24960749252476588Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC464800.247969655086907Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACG464050.24756953193433565Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG463210.24712139400345565Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG459380.24507809843765782Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG453660.24202649252738004Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA445260.23754511321858052No Hit
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT438330.2338479752888209Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACG400040.21342035460620973Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA396100.21131837431136807Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC395860.2111903349025452Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC394910.21068351224262147Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA394360.21039008859740244Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA393600.20998463046946345No Hit
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG385840.20584468958419147Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT371880.1983970639709961Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA371340.19810897530114469No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG370710.19777287185298473No Hit
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT365240.19485464032689742Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT358040.19101345806221212Illumina RNA PCR Primer (100% over 31bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT353160.18840999008281428No Hit
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG351780.18767376348208295Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT333010.17766001471706305Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT330770.17646498023471652Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA329530.1758034432891318No Hit
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT329360.1757127487078823Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT325040.17340803934907112Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACG317320.16928943836526966Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT314010.16752356151858794Illumina DpnII expression Sequencing Primer (96% over 28bp)
TACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGT311980.1664405615189614Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG304770.16259404427890847Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA289540.1544688767940255No Hit
TGGTGTCCTTGGAAAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGA281520.15019022654919548Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT280120.14944332999772889Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT279820.14928328073670036Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA279820.14928328073670036No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC278830.14875511817530612No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT278430.14854171916060138No Hit
AACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGTG277030.1477948226091348Illumina DpnII expression Sequencing Primer (96% over 29bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA273150.14572485216649883Illumina RNA PCR Primer (100% over 45bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT269240.14363887679776Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT269050.14353751226577524Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT261300.13940290635587094Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCAGTTATCACAGTACTGTAGATCGTCGGACTGTAGAACTCTGAACGT250620.1337051526632544Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTG232200.12387812803610113Illumina DpnII expression Sequencing Primer (96% over 29bp)
CACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGT228020.12164810833243662Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC226830.12101324626369002No Hit
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT223330.11914600488502354Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAAC222990.11896461572252451Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGGTGTCCTTGGAAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT219580.11714538912216661Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG219090.11688397532915332Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCTGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGT214240.11429651227585834Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG212430.11333088173431939Illumina DpnII expression Sequencing Primer (96% over 26bp)
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC211060.11259999010895566Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG206610.11022592607036544No Hit
CAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT206540.11018858124279211Illumina RNA PCR Primer (100% over 31bp)
AACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAAC202280.10791588173618663Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA202000.10776650242589332No Hit
TCCTCAAGGAGCTTCAGTCTAGGATCGTCGGACTGTAGAACTCTGAACGT199340.1063473989781068Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT197970.10561650735274308Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTGTA197720.1054831329685526Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC196060.10459752705752794Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGACCCAGTAGCCAGATGTAGCTGATCGTCGGACTGTAGAACTCTGAAC192230.10255423149173007Illumina DpnII expression Sequencing Primer (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)