FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003057989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17121665
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT6419793.749512678819496Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT4860072.8385498723401024Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG4421042.5821320531618857Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA4355572.5438939495662365No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA4251762.4832631639504688No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA3750032.190225074488959No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT3348461.9556859686251309Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG3191591.8640652062752074No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2624261.532713086022884Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGT2611851.52546495916139Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT2433861.4215089478739362Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG2226631.3004751582278942No Hit
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA2165151.2645674354684546No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT1872381.0935735514040252Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC1608810.9396340834842873No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT1582450.9242383845262712Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1516400.8856615288291179Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC1502160.8773445806818438No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1441020.8416354367405272Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG1202290.7022039036507255No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG1031020.6021727442979407Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT982010.5735481917208403Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG973720.5687063728907207Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT910760.531934248217098Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA878930.5133437665086895No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG869380.5077660379408194Illumina DpnII expression Sequencing Primer (95% over 24bp)
CAGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACG811140.4737506545070237Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC773170.4515740729654505Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG764030.4462358070900231Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG700470.40911324920794795Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA675870.3947454876613928No Hit
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG638710.3730419909512305Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA622370.3634985265743723No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC622190.3633933966118365No Hit
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA619390.3617580416390579No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG589890.3445284088901401Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG571210.33361825500031683No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG554680.3239638201074486Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT549520.3209500945147566Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT530390.30977711571859395Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG529390.309193060371173Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT521530.3046023853404444Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA521370.30450893648485705No Hit
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC508760.29714399855387896Illumina DpnII expression Sequencing Primer (96% over 30bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT503620.2941419540681353Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG491170.2868704649927446Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA487650.28481459016982286No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC467600.2731042804540329Illumina DpnII expression Sequencing Primer (100% over 26bp)
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG461560.2695765861556104No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA461090.2693020801423226No Hit
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG448510.26195466387176713Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA445890.2604244388615243No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT430960.2517044925245296No Hit
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA429910.25109123440973763No Hit
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT398010.23245986882700953Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT395260.23085371662160195Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG376370.21982091110882032Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT376060.21963985395111985Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG374470.21871120594872054Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG368620.215294482166308Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC362490.21171422288661762Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG355580.2076784004359389Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAG341440.1994198578234068No Hit
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT331820.1938012453812173Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT326390.19062982484472157Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAATCAGCTAACTACACTGCCTGATCGTCGGACTGTAGAACTCTGAACG315440.18423441879046226Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT314590.18373797174515447Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG313470.183083829756043Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA311810.18211429787932423Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA309040.18049646456696822Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA280610.16389177103979083Illumina RNA PCR Primer (100% over 45bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT276050.16122847865555132Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT269080.15715761288402735Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC260050.15188359309681623Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAA251350.14680231157425402No Hit
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT237110.1384853634269798Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACG234760.1371128333605406Illumina DpnII expression Sequencing Primer (96% over 26bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT233170.13618418535814128Illumina RNA PCR Primer (100% over 31bp)
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG227300.13275578046878034Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT226930.13253967999023458Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA226050.13202571128450416No Hit
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAACTCTGAACGT222530.12996983646158244Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG221920.12961356269965568Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG220230.12862650916251428Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC217990.12731822518429137Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGTG217430.12699115418973564Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT217270.1268977053341483Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGAGAGCTTGCCCTTGTATAGATCGTCGGACTGTAGAACTCTGAACGT214760.12543172641212172Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA212940.1243687456798156No Hit
AACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGTG212800.12428697793117668Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG211130.1233116055009837Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG208010.12148935281703035No Hit
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT205490.12001753334152958Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGA202010.11798502073250469No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA200590.11715566213916695No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA198520.1159466675700056No Hit
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT198210.11576561041230511No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA196420.11472015134042163No Hit
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT196160.11456829695009217Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTAGCCGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC196140.11455661584314376Illumina RNA PCR Primer (100% over 32bp)
TGGCGACGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT196140.11455661584314376Illumina RNA PCR Primer (100% over 35bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT187700.109627188710911Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT186570.10896720616832534Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT181100.10577242341793278Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGA177880.10389176519923733No Hit
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA176980.10336611538655849No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG176970.10336027483308428No Hit
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC171860.10037575200776327Illumina DpnII expression Sequencing Primer (100% over 26bp)
TACATCTGGACAGCCGAATGAAGATCGTCGGACTGTAGAACTCTGAACGT171660.10025894093827908Illumina DpnII expression Sequencing Primer (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)