FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003057997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13405513
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA10498527.831494400848367No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT9322916.954534302417222Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA7949115.9297320438240595No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG3210812.3951414615762934Illumina DpnII expression Sequencing Primer (96% over 26bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT3055042.278942999048227Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2786142.0783538832120785Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA2277191.698696648162588No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2107001.571741417131892Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC1790931.3359652853270143No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1555711.1605001613888257Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA1470131.096660754422453No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT1402861.0464799071844546Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG1323340.9871610284515034Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA1275580.9515338950475077No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT1138600.8493520538900675Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1103060.8228405731283839Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT941890.7026139171249918Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA919250.6857253429988095No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA832690.6211548935128406No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA801770.5980897560578249No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG796010.5937930163508103Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC779630.5815741628089877No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG708240.5283199531416664No Hit
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG631340.4709554942060032Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG594410.44340712660530035Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT489590.3652154154786915Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT469180.34999033606546803Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG460410.34344825147683644Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA438520.3271191486666717No Hit
GGCATTCACCGCGTGCCTTAGATCGTCGGACTGTAGAACTCTGAACGTGT433190.3231431725141738Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC424020.31630270322366627Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT420560.3137216755524388Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG417810.31167028072704117Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA417060.31111080941102365No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA403430.3009433506945986No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG396090.2954679914151737Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA380050.28350276486994563No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC377770.2818019720692524Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT373920.2789300193136958Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC368080.27457360266630604Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG365240.27245507128298635Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG365090.27234317701978283Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT354920.26475674597458526Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT350860.2617281412505437Illumina DpnII expression Sequencing Primer (96% over 28bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT347120.258938244288003Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT343660.2563572166167755No Hit
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG334330.2493973934455175Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT326180.2433178051447938Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT304680.22727962741895819No Hit
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG304520.22716027353820775Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG297860.22219216825197216Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG272720.20343868973906484Illumina DpnII expression Sequencing Primer (96% over 29bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT268440.20024597342899148Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC257940.19241337500474617No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA257190.19185390368872865No Hit
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT254410.18978013001069036Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGTGCCTTATCCATTAGGATCGTCGGACTGTAGAACTCTGAACGTGTAG251630.18770635633265212Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA251530.18763176015718308Illumina RNA PCR Primer (100% over 45bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT249400.18604286161969333Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC249040.1857743153880049Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC237810.17739716488283588No Hit
GCAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAG233290.17402541775163696No Hit
GGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAAC228440.1704075032413903No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT227280.16954218760594988Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT227020.16934823754973047Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG223070.16640168861870486Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG219530.16376098400710215Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA216650.16161261415359485No Hit
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA216530.16152309874303208No Hit
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG208440.15548826814758973Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTGGACTGTAGAAC202200.15083346679832396No Hit
ACAGCCTATGGAATTCAGTTCTCAGATCGTCGGACTGTAGAACTCTGAAC199330.14869255656236358Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA197440.1472826888459994No Hit
ACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGTG184620.13771945915087322Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT182740.13631705105205597Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT180220.1344372274302371Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT178750.13334066365084274No Hit
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC177010.1320426901976821Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT173830.1296705318177678Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGGCCTCGATCAGAAGGACTTGGGCCCCCCAGATCGTCGGACTGTAGAAC170770.12738788884841631No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC168310.12555282293187886Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT168300.12554536331433194Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG166510.1242100917734368Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA163910.12227059121124272No Hit
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT158460.11820509964818204Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACG152080.11344586365325966Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG151690.11315493856893055Illumina DpnII expression Sequencing Primer (96% over 26bp)
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA149410.11145414576823728No Hit
AACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGTG148610.11085737636448527Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGTG148520.11079023980656316Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT140830.10505379391299685Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA139900.10436004948113511No Hit
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT134900.10063024070768496Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG134380.10024234059524614Illumina DpnII expression Sequencing Primer (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)