FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003057998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13456121
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC12618099.377212050932062No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC4609873.425853557648597RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA3584072.6635239085617615No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA2997642.22771480726132RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC2616351.9443567726538726RNA PCR Primer, Index 1 (100% over 36bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA2297861.70766894857738RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1799911.3376143095027164RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1327250.9863540911976044RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC1279730.9510393076875573No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA1277420.9493226168224854RNA PCR Primer, Index 1 (100% over 37bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT1275610.9479775040667366RNA PCR Primer, Index 1 (100% over 35bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1221410.9076984370161356No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1213250.901634282271986RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1172990.8717148129093072No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC1152190.8562571635614752No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA1113970.8278537328848337No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1092800.8121211157361025RNA PCR Primer, Index 1 (100% over 33bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA1049660.7800613564637239No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1033510.7680593835325946No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAAG998860.7423090205565185No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA910310.6765025373954351RNA PCR Primer, Index 1 (100% over 28bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA893200.663787134494406No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA886950.6591424081278698No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA800320.5947627849065864No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG742550.55183065015542RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA693310.515237637949302No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGG683850.5082073801209129Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC673140.5002481770192168No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA638100.4742079831178688No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT567430.42168913314617196RNA PCR Primer, Index 1 (100% over 35bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC560460.4165093343022109RNA PCR Primer, Index 1 (100% over 38bp)
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC537290.3992904047161882No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA526390.39119000193294934No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG500780.372157771173431No Hit
ATAGGCGATAGAAATTGAAACCTTGGAATTCTCGGGTGCCAAGGAACTCC479310.3562022071591063RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC466960.3470242278588309RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC411510.3058162155349228No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC407720.30299965346625524RNA PCR Primer, Index 1 (100% over 31bp)
TCTTTGGTTATCTAGCTGTATGATGGAATTCTCGGGTGCCAAGGAACTCC390960.2905443552417521RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC384040.2854017142087233RNA PCR Primer, Index 1 (100% over 26bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC382470.2842349589454494RNA PCR Primer, Index 1 (100% over 33bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG373660.27768775265918016RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA369490.2745887912274273RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA355800.2644149825941666RNA PCR Primer, Index 1 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC349410.25966621435702014No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC348660.25910884719303584RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC346930.25782318693477857No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC340510.25305212401107274No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA319850.23769851653385102RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA318420.23663580314118754No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA304740.22646942607011336RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT297150.22082887037059196RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC294980.21921622137613062No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG291780.21683812147646414RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA290770.21608753369563188RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC285410.21210421636369053No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC278140.20670147065413577No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA277190.20599547224642226No Hit
GCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC274240.20380316140141724RNA PCR Primer, Index 1 (100% over 31bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA269890.20057043185030812RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA266910.1983558263187437No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC266450.19801397445816665No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG262620.19516768614075336RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC261440.19429076180275132RNA PCR Primer, Index 1 (100% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC254170.18888801609319653No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTTGGAATTCTCGGGTGCC248980.185031035318425No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA247360.18382712224421882RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG243920.18127066485207735RNA PCR Primer, Index 1 (100% over 29bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA243210.18074302393683883RNA PCR Primer, Index 1 (100% over 34bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG239630.178082524674087RNA PCR Primer, Index 1 (100% over 29bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA238150.17698265347049125RNA PCR Primer, Index 1 (100% over 34bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC237060.17617261319216734RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA234790.17448564857584142RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA228340.1696922909655762RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC226360.16822084165265755RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA224380.16674939233973893RNA PCR Primer, Index 1 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC223470.16607312018077125No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGAATTCTCGGGTGCC220080.16355382059956208No Hit
NTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC218380.16229045502786427No Hit
TCCCTGTAGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA213340.1585449476858896No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA211280.15701404587547926RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC204090.15167075266341615No Hit
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC197250.14658756412787904RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA195370.145190430436825RNA PCR Primer, Index 1 (100% over 32bp)
TCCAGCATCAGTGATTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG193140.14353319206924492RNA PCR Primer, Index 1 (100% over 29bp)
TGAGACCTCTGGGTTCTGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCA190780.1417793433932409RNA PCR Primer, Index 1 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATTGGAATTCTCGGGTGCCAAGGAACTCCAG189220.1406200196921535RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGAGATGGAATTCTCGGGTGCCAAGGAACTCC186510.13860606633962344RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC185490.13784804699660475RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA184500.13711232234014542RNA PCR Primer, Index 1 (100% over 28bp)
TTATAAAGCAATGAGACTGATTTGGAATTCTCGGGTGCCAAGGAACTCCA182610.1357077570869049RNA PCR Primer, Index 1 (100% over 28bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG181380.13479367493797062RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCC175110.1301340854470616No Hit
GTCTCTGGCGCAATGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA170570.12676015621440978RNA PCR Primer, Index 1 (100% over 32bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG169620.1260541578066963RNA PCR Primer, Index 1 (100% over 29bp)
CGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCAAG168780.12542990658303385No Hit
TCTTTGGTTATCTAGCTGTATGTGGAATTCTCGGGTGCCAAGGAACTCCA166650.12384698383731835RNA PCR Primer, Index 1 (100% over 28bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC166210.1235199951011142RNA PCR Primer, Index 1 (100% over 36bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA165880.1232747535489611RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGAGTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAAG164760.12244241858407783No Hit
TCCAGCATCAGTGATTTTGTTGTGGAATTCTCGGGTGCCAAGGAACTCCA161610.12010147649534364RNA PCR Primer, Index 1 (100% over 28bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC161350.11990825587849575RNA PCR Primer, Index 1 (100% over 38bp)
AGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA161170.11977448775913951RNA PCR Primer, Index 1 (100% over 40bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG160510.11928400465483328RNA PCR Primer, Index 1 (100% over 39bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA158730.1179611865856438No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG157100.1167498419492512RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA155890.11585062292468981RNA PCR Primer, Index 1 (100% over 28bp)
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATTCTCGGGTGCCAAG154010.11445348923363576No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA153230.11387382738309204RNA PCR Primer, Index 1 (100% over 28bp)
AAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG151640.11269220899544528RNA PCR Primer, Index 1 (100% over 39bp)
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG151420.1125287146273432Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA150010.11148086435905265No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA148050.11002427817050693No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTTTGGAATTCTCGGGTGCCA146130.10859741823070704No Hit
AACATTCATTGCTGTCGGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCA146080.10856026041977476RNA PCR Primer, Index 1 (100% over 28bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT145890.10841906073823207RNA PCR Primer, Index 1 (100% over 35bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG141120.10487420557529172RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)