FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003057999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003057999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13456121
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA11725368.71377419986042No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG4470913.32258456950558Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA3249222.414678048748224No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT2498581.8568352647839599Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT2465631.8323482673795812Illumina RNA PCR Primer (100% over 31bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT2232131.6588212903257928Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT1662331.2353708769414307Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC1274060.9468256119278355Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1195800.8886662062566173Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC1144130.8502673244391902Illumina RNA PCR Primer (100% over 32bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA1103750.8202586763302738No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1092470.8118758741839495Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA1091860.8114225488905755No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1069920.7951177014534871No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA1048240.7790060746332469No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1047920.7787682646432802Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG1002910.7453188032420338No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT942020.7000680210886926No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA924110.6867580932127468No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA916240.6809094537720045No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA817130.6072552409420219No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC807580.6001580990539548No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT795070.590861214758696Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG718360.5338537012263788Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA687120.5106375009558847No Hit
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC650470.48340082554251707Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA626760.4657805915984257No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC610500.45369687148324545No Hit
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC567150.42148104940495107No Hit
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC524220.389577352938488Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG521910.38786066207341624Illumina RNA PCR Primer (100% over 33bp)
AGCGCCGAATCCTAACCACTAGACTACCAGGGAGATCGTCGGACTGTAGA506180.37617081475411823No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC481920.35814184488977174No Hit
AGGTTTCAATTTCTATCGCCTATGATCGTCGGACTGTAGAACTCTGAACG444870.33060790698894577Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC434070.3225818198275714Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT392040.2913469639578895No Hit
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA382610.28433900081605984Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG376830.28004355787228724Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT356140.2646676557085062Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA341280.25362435429943No Hit
CCACGGGAACGTGGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA335510.24933634291784387Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG319940.23776540059352916Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA317080.23563997380820226No Hit
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA316170.23496370164923455No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC314990.23408677731123254Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA306070.22745782384091226No Hit
AGCGACGCTCAGACAGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAG302770.2250054083193812Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGCCGAATCCTAACCACTAGACTACCAGGGAGATCGTCGGACTGTAGAA294370.21876289608275667No Hit
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT279730.20788308904178257Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT279700.20786079435522317Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA276770.20568334663459104No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG274100.20369911953080685Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT271010.20140276681519137Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT267360.19869024661713433Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG262830.19532374894666893Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGCGACGCTCAGACAGGCGATCGTCGGACTGTAGAACTCTGAACGTGTA261460.19430562492712425Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG259010.1924848921914421No Hit
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG253830.18863534297885698Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG247390.1838494169307782Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAG241050.17913780650456398No Hit
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT238210.17702724284361Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC237910.17680429597801625Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA236410.1756895616500476No Hit
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA236110.17546661478445386No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA235070.17469373231706226No Hit
AGCGACGCTCAGACAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT232370.17268721052671868Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC229690.170695551860748Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTCCAGAGTGCTAACCATTACACCATGGAACCGATCGTCGGACTGTAGA226900.16862214601072628No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT225490.16757429574243574Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG221320.16447533431068284Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT219460.1630930637440017Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT214740.15958536639199367Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT210400.15636006840307098Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG209430.1556392068709846Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG206550.15349891696128476Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA205350.1526071294989098No Hit
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA203020.15087557550946518No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT200900.14930008432593614Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACTACAGGGAGATCGTCGGACTGTAGAA199740.14843802311230703No Hit
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG196700.14617882820762387No Hit
AAGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAG189150.14056799875684828Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA183070.13604960894748197No Hit
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG181730.13505377961449663Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG178850.1329134897047968Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG176930.13148662976499692Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT174000.12930918204436478Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG172600.1282687633382607Illumina DpnII expression Sequencing Primer (96% over 26bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT171010.1270871449506139Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG166400.1236611947826569Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG165420.12293290168838404Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA165280.12282885981777364No Hit
AGCGACGCTCAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT164160.12199652485289038Illumina RNA PCR Primer (100% over 31bp)
GTCCATTGCGCCAGAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAG162200.12053993866434465Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT161990.12038387585842905Illumina DpnII expression Sequencing Primer (96% over 28bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT159520.11854827999837397Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAACCCTTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG157300.11689847319298036Illumina RNA PCR Primer (100% over 34bp)
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT157110.11675727351143766Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGTG156150.11604384354153771Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGATCGTCGGACTGTAGAACT155880.11584319136250336No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCCGTCGGACTGTAG155710.11571685480533357No Hit
TAATGATCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT152230.11313067116444629Illumina RNA PCR Primer (100% over 35bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTG150590.11191189496586722No Hit
AACTCAGTAATGGTAACGGTTTGATCGTCGGACTGTAGAACTCTGAACGT150440.11180042153307035Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAATGATCCTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG148350.11024722503610067Illumina RNA PCR Primer (100% over 34bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT145280.10796573544485816Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCAGCACGCTTCCGCTGCGCACTCTGCTGATCGTCGGACTGTAGAACT145260.10795087232048522No Hit
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT145140.10786169357424773Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG144110.10709624266904258Illumina RNA PCR Primer (100% over 33bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT141390.10507485775432608Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA140720.10457694308783341No Hit
GTCTGATGCTCTACCGACTGAGCTATCCGGATCGTCGGACTGTAGAACTC140210.10419793341632407Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCGACGCTCAGACAGGCGTAGATCGTCGGACTGTAGAACTCTGAACGTG139400.103595976879221Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTGATCGTCGGACTGTAGAACT138340.10280823128745646No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGCGGACTGTAGAA138220.10271905254121898No Hit
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC135090.1003929735768577Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAAC135090.1003929735768577No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)