FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003058003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003058003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16885071
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA292820617.341982156900613No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA7269934.30553712211219No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT2914221.725915159018283No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG2632261.5589274098995498No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA2487631.4732718624635928No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA2482491.4702277532620383No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA2398431.4204441307945936No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG2264401.3410663182879126Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA2204031.3053128411482546No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC2134441.2640989190984153No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2032571.203767517471499Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA2008881.1897373721437121No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT1764841.045207331375746Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA1528240.9050835498411585No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA1498860.8876835637824679No Hit
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC1341950.7947553196548597Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG1258150.7451256793649254No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA1189470.704450694936373No Hit
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT1104070.6538734720156048Illumina RNA PCR Primer (100% over 31bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT1047800.6205481753674592Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC1046780.6199440914403025No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA1045000.6188899057634996No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1044430.6185523294512649Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA1015490.6014129286160538No Hit
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA997330.5906578657560871No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC979750.5802463015997978No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA875620.5185764395068283No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT830530.49187237649163573Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA806340.4775461115917132No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT784240.4644576265033176Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG734740.43514178886188876Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC732810.4339987673134451No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA721240.4271465604142263No Hit
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA681670.40371165747541127No Hit
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA653500.387028280781289No Hit
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC595660.3527731686766375Illumina RNA PCR Primer (100% over 32bp)
GCAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAG567650.3361845502455986No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT563860.3339399638888104No Hit
AGTCCAGAGTGCTAACCATTACACCATGGAACCGATCGTCGGACTGTAGA543730.3220181899146293No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT542170.3210942968495661Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG505000.2990807678570022Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAAACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTA500650.2965045275794221No Hit
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC497810.2948225684096916Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA464010.2748048853333219No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC425510.25200367827887726Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA414280.24535283268871064No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG394350.2335495065433838Illumina DpnII expression Sequencing Primer (95% over 24bp)
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG385730.22844440511976524No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCCGTCGGACTGTAG384600.2277751748867387No Hit
AGCGCCGAATCCTAACCACTAGACACAGGGAGATCGTCGGACTGTAGAAC377580.22361765609395426No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT372550.22063869319826965Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACATGCGATCGTCGGACTGTAGAACT353290.2092321672796046No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG343880.2036591969320117Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGCGGACTGTAGAA330920.1959837776222558No Hit
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC316960.18771611916822856No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG307050.18184702924850005No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA294710.17453879820819232Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA291390.1725725642492116No Hit
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG288710.17098536334256456Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG284570.16853349328528142No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA281570.16675677585246754No Hit
GCAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAG278640.16502151515975266No Hit
GTCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGT271850.16100021137015058No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC269300.1594900015522588Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACCAGGAATCCTAACCGCTAGACCATATGGGAGATCGTCGGACTGTAGA261950.1551370438418648No Hit
GGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAAC261670.15497121688146887No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT261010.1545803390462498Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT257190.15231798551513348No Hit
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC256040.15163691049922146Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA252220.14937455696810514No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTGGACTGTAGAAC241040.1427533233351521No Hit
AGAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAA240830.14262895311485513No Hit
TCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTA235000.13917619890375352No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT223660.13246020700771705Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT216350.1281309388630939Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT210010.12437614268841393Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA209570.12411555746493455No Hit
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG208400.12342263766613715Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG207110.12265864917002718Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG203940.12078125108268718Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAAGGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA202800.12010609845821792No Hit
GTCCATTGCGCCAGAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAG202770.12008833128388978Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT200740.11888608582101906Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA199570.11819316602222166No Hit
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA198970.11783782253565887No Hit
CAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGA198650.11764830600949204No Hit
ACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGAA189650.11231815371105043No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC189140.11201611174747206Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCCAGCACGCTTCCGCTGCGCACTCTGCTGATCGTCGGACTGTAGAACT186960.11072503041296067No Hit
AAGCGAGAATCATACCCCTAGACCAAAGCCGATCGTCGGACTGTAGAACT186750.1106006601926637No Hit
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA184050.10900161450313119No Hit
AGAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAG182750.10823170361557852No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGGA180970.10717751793877563No Hit
AGCGCCGAATCCTAACCACTAGACAACAGGGAGATCGTCGGACTGTAGAA176570.10457166570398194No Hit
AACGCAGCCTTAGACCGCTCGGCCATCCTGACGATCGTCGGACTGTAGAA176010.10424001178319002No Hit
GCGCCGAATCCTAACCACTAGACACAGGGAGATCGTCGGACTGTAGAACT175140.10372476372767399No Hit
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG174860.10355893676727802Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT174440.10331019632668409Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT174420.10329835154379867Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG173650.10284232740270978Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTGGACTGTAGA171640.10165192672272447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)