FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003058005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003058005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16977373
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA14770388.700038574872568No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG6933014.083676549958583Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT5654663.33070375493311Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT4273072.516920609566627Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT3331831.9625121036099047Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT3270111.926157833723745Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA2974281.751908260482938No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2395941.411254850794643Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT2213291.303670479525896Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1765211.039742721091184Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA1690830.9959314671356988No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG1513540.8915042392012003Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA1461850.861057832681181No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA1432190.8435875208726344No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT1375520.8102077983443021No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT1284850.7568014203375281Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG1255500.7395137045054025Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA1198670.7060397388924659No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC1063230.6262629677748142Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC986170.5808731421522045No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG963220.5673551497042564No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG918790.5411850231481632Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG874340.5150031162064944Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG779300.45902272395146176No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT772580.4550645143980756Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA767210.4519014808710394No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT734100.4323990525507097Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG717530.42263900310136315Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG698200.4112532604425903Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT684600.4032425982512135Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG662350.3901369192984097Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT660990.38933585307927204Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA642930.3786981649045468No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT626870.3692385152873769Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT609510.35901314060779604Illumina RNA PCR Primer (100% over 31bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC605380.3565804909864441Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA549680.3237721171585262No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA546650.3219873887438298No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC539530.31779357147893256Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG526730.310254124710578Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA508830.29971067962045717No Hit
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG507210.2987564683888373Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT500500.2948041490282389Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC492440.2900566536412907Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT481930.28386606102133705Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG481470.2835951121530993Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC451680.2660482278383116No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT449700.26488196966633176Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC426710.25134041644723243Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC422440.24882530412685167No Hit
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT409770.24136242986473821Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG401970.23676807949027215Illumina DpnII expression Sequencing Primer (96% over 29bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG396960.23381709290359584Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC395150.2327509680090082Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG383200.22571218762761472Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG380230.22396280036964494Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA375160.22097647262624198No Hit
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT371100.21858505435440453Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT351730.20717575092448048Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT349480.20585045754723066Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG348810.2054558146304496Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT340100.20032545671229582Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA339380.1999013628315759No Hit
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC333310.19632601580939524Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA324380.19106607364991038No Hit
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG322340.18986447432120387Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA320670.18888081212564511Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT315470.18581791187600108Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGT310640.1829729487595048Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG304650.17944472327962635Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA298680.1759282781853235No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA298490.17581636452235572No Hit
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC293260.17273579369434836Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT289120.17029725388020867Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT283470.16696929495511467Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG283000.16669245589408915Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGACCCAGTAGCCAGATGTAGCTGATCGTCGGACTGTAGAACTCTGAAC278860.16425391607994946Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT278070.1637885908497151Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA271340.15982449110354116No Hit
CACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGT264270.15566012480258282Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGT263150.15500042321035182No Hit
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC260130.15322158498844315Illumina RNA PCR Primer (100% over 32bp)
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT251210.14796753302174606Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC247030.14550543243645528No Hit
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG246060.14493408373604091Illumina DpnII expression Sequencing Primer (96% over 29bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT243140.14321414744201003Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT242240.14268403009111008Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG235690.13882595381511617Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT235580.1387611616944506Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT229490.13517403428669442Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT229320.1350739010093022Illumina DpnII expression Sequencing Primer (96% over 28bp)
CACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGT228810.13477350117712558Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT228360.1345084425016756Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT216360.1274402111563432Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC214820.12653312146702556Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT211770.12473661266675357Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACG207330.12212136706898058Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT201250.11854012985401216No Hit
GCAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAG201230.1185283494684366No Hit
GCAAGGTCGGTTCTACGGGTGGATCGTCGGACTGTAGAACTCTGAACGTG197350.11624295466677913Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT194960.11483519859050043Illumina DpnII expression Sequencing Primer (96% over 30bp)
TACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGT181620.10697768141160591Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACG181050.10664194042270261Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCCGTCGGACTGTAG181050.10664194042270261No Hit
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG180590.10637099155446487Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAGTTCGGATCTACGGGTTGATCGTCGGACTGTAGAACTCTGAACGTG179920.10597634863768382Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGGCCTCGATCAGAAGGACTTGGGCCCCCCACGATCGTCGGACTGTAGAA178380.10506925894836615No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGCGGACTGTAGAA174740.10292522877361533No Hit
AACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAAC172790.10177664117999882Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCAGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTG172080.10135843749206665Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG171920.10126419440746222Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGT171640.10109926900940447Illumina DpnII expression Sequencing Primer (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)