FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003058011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003058011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20024797
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA12068766.026907538688158No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA5115132.554397929726828No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA3637131.8163130442720592No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG3515161.7554035628925477Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2667281.3319885340161002Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG2335001.166054267616296No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT2049351.0234061299098314Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT1932160.9648836889582452No Hit
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG1843550.9206335524899454No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA1839180.9184512582075114No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT1759240.8785307536450931Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA1722710.8602883714626421No Hit
CAGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACG1715480.8566778479701942Illumina DpnII expression Sequencing Primer (96% over 26bp)
TATTCTCGCACGGACTACAACCACGACCAATGATCGTCGGACTGTAGAAC1692340.8451221752709903No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA1643220.8205925882794217No Hit
GTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGAA1501200.7496705210045325No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1421190.7097150597831279Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA1419010.7086264095461242No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1405670.7019646691050102Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA1369890.6840968225545557No Hit
AATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGT1301690.650039049084992No Hit
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA1242340.6204007960729889No Hit
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC1224860.6116716189432533No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1222510.6104980739629969Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG1191860.5951920511353997No Hit
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT1178160.5883505335909274Illumina RNA PCR Primer (100% over 31bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC1161570.5800658054111609No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG1127740.5631717515038979Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC996760.49776284873199966Illumina RNA PCR Primer (100% over 32bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC778920.3889777259664605Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG763670.38136216811586154No Hit
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG762150.38060310923501495No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA747890.3734819384186516No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG720510.3598088909465599Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAA717690.35840063697025243No Hit
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA701440.3502856982769913No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG699060.34909717187145517No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA680840.3399984529181494Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA644340.32177105216097823No Hit
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA611050.3051466639087527No Hit
CAATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAGA607040.3031441467296772No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA605370.30231018072243127No Hit
AGAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAG571240.28526631256236956No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA539510.26942095842469715No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC538680.2690064723252875Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCATAGGATGACAAAGGGAAGATCGTCGGACTGTAGAACTCTGAACGT519150.2592535644680942Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTGATCGTCGGACTGTAGAACT510030.25469921118301475No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG504980.2521773379275705Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAA486000.24269908953384145No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT473860.23663660610392206Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT457820.22862653738761995Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA451430.22543549380300834No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC450750.22509591482999802No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA439290.21937301037308893No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG434630.21704589564628296Illumina DpnII expression Sequencing Primer (95% over 24bp)
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG432150.21580743115648063No Hit
GTCAGAGCGGTCAAGTTAAGTTGAAATCTCCGATCGTCGGACTGTAGAAC416850.2081669042637486No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA416360.2079222076508441No Hit
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC401380.20044148262776396Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC383460.19149257792725688No Hit
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA381730.19062864906945123No Hit
CAACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAG380060.18979468306220534No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT373720.18662860851972682Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT365760.1826535370121355Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTG365010.18227900138013883No Hit
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG364510.18202931095880773Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG363560.1815548991582786Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGTGTTTATGGGGTGATGTGAGCCCGTCTAAGATCGTCGGACTGTAGAAC354690.17712539108386466No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC354550.17705547776589195Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTCAGAGCGGTCAAGTTAAGTTGAAATCTGATCGTCGGACTGTAGAACTC352620.17609167273955387Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGGA347490.17352984901669663No Hit
TGGCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGT341040.1703088425815253No Hit
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG340270.1699243193326754Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA331950.16576947072172568No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG325040.16231874909892968Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC323660.16162960353605582Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGACTAGTCAAGTGCAGTAGTGAGAAGGATCGTCGGACTGTAGAACTCTG320790.16019638051761523Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA317630.1586183370548026No Hit
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA312210.15591169288757334Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAG308770.1541938227888153No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG306600.15311016636023828Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA305610.15261577932600265No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT301420.15052337359524792Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAATCAGCTAACTACACTGCCTGATCGTCGGACTGTAGAACTCTGAACG295580.14760698947410056Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC284160.1419040602508979Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG271980.13582160158727202Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT262610.13114240309152697Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG260340.13000880857868374Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG259900.12978908100791234Illumina DpnII expression Sequencing Primer (96% over 26bp)
TATTCTCGCACGGACTACAACCACGACCAGATCGTCGGACTGTAGAACTC254760.12722226347662852Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG247050.12337203717970274Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGA246560.12312734056679825No Hit
AGCGCCGAATCCTAACCACTAGACACAGGGAGATCGTCGGACTGTAGAAC243310.12150435282814603No Hit
TACATCTGGACAGCCGAATGAAGATCGTCGGACTGTAGAACTCTGAACGT243060.12137950761748047Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT238420.11906238050752775Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAAACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTA234720.1172146713896775No Hit
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA229880.11479766811119234No Hit
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT226490.11310476705456739Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT226200.11295994661019534Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTA226040.1128800456753694No Hit
GGTGCATTGGCCGGGAAACGAACCCGGGCCTCCCGCGGATCGTCGGACTG221330.11052796190643031No Hit
AACAATCAATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGAC221070.11039812288733813No Hit
GGTATTCTCGCACGGACTACAACCACGACCAATGATCGTCGGACTGTAGA212280.10600856528033718No Hit
CAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGA211750.1057438934337262No Hit
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC211700.10571892439159308Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG208700.10422078186360642Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTGGACTGTAGAAC207860.10380130195577013No Hit
GTCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGT206290.10301727403279044No Hit
AAAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCT206280.10301228022436382Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG205830.10278755884516583Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG202890.10131937916773887Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCTAGACCAATGGGACTTAAACCCACAAACACTTGATCGTCGGACTGTAG201730.1007400973902507No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)