FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003058013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003058013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25025926
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA318703512.734933364703469No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG8683863.4699455276899647Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA8355513.3387415914200336No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA5801112.318040099695012No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT3639891.45444767957837Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT3268431.3060176075003178Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2979711.1906492491027105Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA2857401.1417759326867665No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC2650131.0589538225278856No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT2599091.0385589728028446No Hit
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC2525611.009197421905587Illumina RNA PCR Primer (100% over 32bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT2487000.9937694213592736Illumina RNA PCR Primer (100% over 31bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA2452770.98009160580112No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG2423710.9684796478659771No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT2375190.9490917538875484Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2308720.9225312981425742Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2050990.8195460979146186Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA1873470.748611659764358No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG1781030.7116739656306823Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC1772240.7081616080859505Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA1624020.6489350284181292No Hit
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA1328840.5309853469557929No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA1306030.5218707991064946No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA1230770.4917979858167886No Hit
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA1224980.48948438511326214No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA1142050.4563467501662076No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1113250.4448386844906358Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT1040370.41571688496161946Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC1028490.41096980787044607Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA996830.3983189273395918No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG993690.3970642285124635No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC962430.3845731822271032No Hit
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC944580.37744057902193107Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG920410.36778259473795294Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG788200.31495338074603113Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGGA777270.31058590998790614No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA772600.3087198451717631No Hit
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG761390.30424049044179224Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAAACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTA737920.2948622160874287No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC734340.29343169959025694No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA696880.27846322249973887No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG695660.27797572805098203Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA677950.2708990668317328No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT677520.27072724501782675No Hit
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC673700.2692008279733585Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA617650.24680405432350436No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT542290.2166912824724248Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA536580.2144096486179972No Hit
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG516760.20648986175376688No Hit
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA516170.20625410624166315No Hit
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC502870.2009396175789859No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG499880.19974485659391786Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT498520.19920142015923806Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC466120.1862548462742198Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG458630.18326195002734363Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC457170.18267855503129035Illumina DpnII expression Sequencing Primer (100% over 26bp)
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC457030.18262261304536745No Hit
GTCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGT454850.18175151640742485No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT448680.17928607317067907Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT445730.17810729561016045Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA443110.17706038130217439Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA441540.17643303188861023No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT438760.17532218388242657Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG438540.17523427504740485Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCCGTCGGACTGTAG437200.17469883032499978No Hit
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG424290.1695401800516792Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG423290.16914059443794408Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTG417760.1669308859939888No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC411430.1644015090590454Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA394410.15760056191327346No Hit
AGTCCAGAGTGCTAACCATTACACCATGGAACCGATCGTCGGACTGTAGA392210.15672147356305616No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT388270.15514710624493974Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG385040.15385644471257529Illumina DpnII expression Sequencing Primer (96% over 26bp)
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG382730.1529334019448471No Hit
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT366970.14663593267238145Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT364080.1454811302486869Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGCGGACTGTAGAA354560.14167707520592843No Hit
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT350700.14013467473691082Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG349530.13966715956884074Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGTTTCAATTTCTATCGCCTATGATCGTCGGACTGTAGAACTCTGAACG348760.13935947864626466Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA341760.13656237935011875No Hit
AACGCAGCCTTAGACCGCTCGGCCATCCTGACGATCGTCGGACTGTAGAA333960.13344561156298473No Hit
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT332110.13270637817757472Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA332050.13268240304075063Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT327450.130844309217569Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACATGCGATCGTCGGACTGTAGAACT326550.1304846821652074No Hit
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG320910.12823101930374126Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGCCGAATCCTAACCACTAGACACAGGGAGATCGTCGGACTGTAGAAC316390.12642489232965845No Hit
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA304700.12175373650509475No Hit
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA302370.1208227020250919No Hit
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT300790.12019135675539039Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG299770.11978377942938057No Hit
CACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGT291870.11662705308087301Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG282200.11276306019605427Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA280430.11205579365974311No Hit
AACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAAC280110.11192792626334785Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACCAGGAATCCTAACCGCTAGACCATATGGGAGATCGTCGGACTGTAGA277590.11092097051673533No Hit
AGCGACGCTCAGACAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT270430.10805993752239176Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG270000.10788811570848567Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT267050.10670933814796704Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAAC266910.10665339616204413Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCGATCG265700.10616989756942462No Hit
CGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA264440.10566641969611834No Hit
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG259920.10386029272203554No Hit
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG259790.10380834659224998Illumina RNA PCR Primer (100% over 33bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG257840.10302915464546647Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAA255010.10189832735859602No Hit
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT254630.1017464848253767Illumina DpnII expression Sequencing Primer (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)