FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003058015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003058015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23073432
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA273684711.861464735718554No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA7180333.111947108691936No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT5941742.5751435677189245No Hit
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA4789332.075690343768539No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA4624092.0040755098764675No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG3719861.6121832244115224Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA3111371.348464329017027No Hit
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT2823591.2237407941740093Illumina RNA PCR Primer (100% over 31bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT2705561.1725867222526756Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC2503181.0848754532919074No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT2460641.0664386641744497Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC2444861.0595996295652939Illumina RNA PCR Primer (100% over 32bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC2340101.0141967610193403Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2252580.9762656894735036Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG2198020.9526194456030642No Hit
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA2008590.8705206923703418No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1771690.767848493453423Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA1766530.7656121551401629No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT1742760.7553102633366375Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT1736800.7527272059050427Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC1603570.6949854707353461Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA1521570.6594467611060201No Hit
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA1314680.5697808631156388No Hit
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG1269660.550269244731343Illumina RNA PCR Primer (100% over 43bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1212810.5256305173846699Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1135740.4922284643220826Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG1116590.48392887542694124No Hit
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC1029560.4462101693410846Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG984510.42668554898985117Illumina DpnII expression Sequencing Primer (96% over 26bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA962100.41697307968749514Illumina RNA PCR Primer (100% over 45bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA949900.41168561313288804No Hit
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA927740.40208149355501166No Hit
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA781050.3385062092193307No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC779080.33765241339043106No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA776790.3366599299141974No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG774710.3357584602065267Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA765710.3318578701252592No Hit
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA733520.31790675960125914No Hit
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC720270.31216422420383755Illumina RNA PCR Primer (100% over 39bp)
AGCGCCGAATCCTAACCACTAGACACAGGGAGATCGTCGGACTGTAGAAC691350.29963032807603135No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG680960.295127313526657Illumina DpnII expression Sequencing Primer (95% over 24bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC648150.28090749568594736Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA624590.2706966176509849No Hit
AGAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAA614090.2661459292228395No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT538560.23341131046304683Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTA520750.2256924760911164No Hit
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC515630.22347347373377313No Hit
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA499560.21650875344422105No Hit
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA472060.20459028375145924Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAG465560.2017731909149883No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT443620.19226441909465397Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA438210.1899197310569143No Hit
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT430250.18646987582948216No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC429630.1862011685127726Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT428040.18551206426508202Illumina RNA PCR Primer (100% over 38bp)
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC424370.18392149030972074No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC415910.18025493563332928Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG400370.17351991675967407Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG396260.17173864728922858Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG372990.16165345493466252Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCCGTCGGACTGTAG371880.16117238215797286No Hit
AGTCCCATGCTCTACCGACTGAGCTAGCCGGATCGTCGGACTGTAGAACT366830.15898371772348388No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT366790.15896638176756714Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTGGACTGTAGAA364840.15812125391662582No Hit
AGTCCAGAGTGCTAACCATTACACCATGGAACCGATCGTCGGACTGTAGA361810.15680805525593247No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT357600.1549834458956951Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGT353570.15323684833708312No Hit
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGCGGACTGTAGAA351060.1521490171033074No Hit
AGGCGAGAATTCTACCACTGAACCACATGCGATCGTCGGACTGTAGAACT349090.15129522127440773No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGGA348940.15123021143971993No Hit
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG348890.15120854149482402Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT348250.1509311662001561Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACAACAGGGAGATCGTCGGACTGTAGAA344840.1494532759582536No Hit
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT335740.14550934598719428Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA328120.14220684638505446No Hit
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG326610.14155241404919736Illumina RNA PCR Primer (100% over 33bp)
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG324960.14083730586763166Illumina RNA PCR Primer (100% over 40bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG321510.13934207966981244Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGACGCTCAGACAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT314620.13635596126315322Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT313720.13596590225502647Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA305900.13257672287330294Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCC303080.13135453798117244Illumina RNA PCR Primer (100% over 42bp)
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC300810.13037072248289722No Hit
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG292380.12671716977344333Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG290940.1260930753604405Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT287460.12458484719568377Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGTGTCAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT287140.12444615954834981Illumina RNA PCR Primer (100% over 35bp)
CTCGGGGCAGCTCAGTACAGGAGATCGTCGGACTGTAGAACTCTGAACGT286790.12429446993407829Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACGACAGGGAGATCGTCGGACTGTAGAA279080.1209529644311258No Hit
ATCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC277530.12028119613935195Illumina RNA PCR Primer (100% over 39bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG272350.11803618984813355Illumina DpnII expression Sequencing Primer (96% over 29bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT270310.11715205609637959Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG267430.115903867270374No Hit
AGCGCCGAATCCTAACCACTAGACCACAGGGGAGATCGTCGGACTGTAGA262660.11383655452730222No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC259690.11254935980048394Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG259400.11242367412008754Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA257380.11154820834629196No Hit
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA248540.10771696208869144No Hit
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT241830.10480885548365758Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT241250.10455748412286478Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA239840.10394639167679953No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTGGACTGTAGAAC238730.10346531890010988No Hit
TGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC236240.10238615564429254Illumina RNA PCR Primer (100% over 41bp)
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA236180.10236015171041742No Hit
AAGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT232000.10054854431711761Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)