FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003058017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003058017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24373651
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA286054911.736235166409825No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA8467783.474153297755843No Hit
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT4388321.8004360528506786No Hit
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT3888361.5953129057275826Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG3822771.5684026984713944Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA3411171.399531814088911No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT3283531.3471637876492117Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA3265911.3399346696151513No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA3209441.3167662079021318No Hit
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC3043921.248856808526552No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2531921.0387939008398865Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG2301940.9444379096098487No Hit
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC2174730.8922463031902771Illumina RNA PCR Primer (100% over 32bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA2091890.8582587811731611No Hit
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT2000850.820906970400126Illumina RNA PCR Primer (100% over 31bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1822870.7478854932320151Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC1677710.6883293766699129Illumina DpnII expression Sequencing Primer (96% over 30bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT1650360.6771082428315726Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA1569240.6438264008949665No Hit
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA1496610.6140278286580866No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1486450.6098593928336793Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1435270.5888613076473442Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT1417300.5814885919224821Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC1407190.5773406700539038Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC1287210.528115381647173Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA1261150.517423507869215No Hit
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA1145360.4699172889609357No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG1101440.45189783016093893No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA1100930.4516885878114854No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC1075510.44125929266813574No Hit
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA993400.40757127440611995No Hit
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA911610.37401454546140833Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA886960.36390116523782173No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA854460.35056709394911745No Hit
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC824740.338373598604493Illumina RNA PCR Primer (100% over 39bp)
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA807180.33116909731742694No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG800470.3284161244452052Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG740730.3039060500209837Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA722990.296627698492934No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC697910.28633789824922No Hit
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT663900.27238430549448667Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT661660.2714652802733575No Hit
GCCCAGCACGCTTCCGCTGCGCCACTCTGCTGATCGTCGGACTGTAGAAC651440.2672722277019557No Hit
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG642630.26365766868492535Illumina DpnII expression Sequencing Primer (96% over 29bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA629950.2584553294867478Illumina RNA PCR Primer (100% over 45bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT617940.2535278772966758Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT617470.25333504611188534Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG605820.24855529440378052Illumina RNA PCR Primer (100% over 43bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG576630.23657924699094116Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC569830.23378934899822762Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGAA565480.23200463484112413No Hit
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC558840.22928038150706267Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG552220.22656433375533278Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGCCGAATCCTAACCACTAGACACAGGGAGATCGTCGGACTGTAGAAC528560.216857129857156No Hit
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA511790.20997674907218453No Hit
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG473770.19437793705998335Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG472010.19365584581481043Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT464700.1906567054726434Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC464690.19065260268147763Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTCCAGAGTGCTAACCATTACACCATGGAACCGATCGTCGGACTGTAGA462830.18988948352464718No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT446180.1830583362336648Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT432860.17759341840087886Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACATGCGATCGTCGGACTGTAGAACT429550.1762353945250139No Hit
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT419630.17216542568858478Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT403430.1655189040000614Illumina RNA PCR Primer (100% over 38bp)
TCTGATCTGAGGTCGCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA387920.1591554749019751Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAA378080.155118328394872No Hit
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG377010.15467932974013618Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA374590.15368645427802344No Hit
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT369320.15152428333367046Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACGCAGCCTTAGACCGCTCGGCCATCCTGACGATCGTCGGACTGTAGAA368640.15124529353439908No Hit
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA358640.1471425023686439No Hit
AAAACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTA356230.1461537296976969No Hit
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT356190.14613731853303388Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGTTTCAATTTCTATCGCCTATGATCGTCGGACTGTAGAACTCTGAACG353560.14505828445644028Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGCGGACTGTAGAA351150.14406951178549326No Hit
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG346650.14222325576090344Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC344420.14130833333094003Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA343310.14085292351154122Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCCGTCGGACTGTAG336170.137923530619192No Hit
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG324610.13318070403157903Illumina RNA PCR Primer (100% over 33bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT318680.13074774887028617Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG312780.12832710208249062Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG297460.12204162601655369Illumina RNA PCR Primer (100% over 40bp)
GCAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAG296130.12149595479150825No Hit
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG294880.12098310589578887Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC291510.11960046527292936Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTGGACTGTAGAAC280030.11489046101464241No Hit
AGGCGAATGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA279020.11447607910690114No Hit
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA275080.1128595793875936No Hit
AGAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAG268580.11019276512985272No Hit
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG267440.10972504693695663Illumina DpnII expression Sequencing Primer (96% over 29bp)
AAGCGAGAATCATACCCCTAGACCAAGCCGATCGTCGGACTGTAGAACTC267430.1097209441457909Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGCGAGAATCATACCCCTAGACCAAAGCCGATCGTCGGACTGTAGAACT267140.10960196320198398No Hit
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC260550.1068982238237513No Hit
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG258450.10603663767894272Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT254300.10433397934515433Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACTC249860.10251234006755901Illumina DpnII expression Sequencing Primer (100% over 21bp)
AACCAGGAATCCTAACCGCTAGACCATATGGGAGATCGTCGGACTGTAGA244090.10014502956491828No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)