FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003058019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003058019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18161826
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG10007105.509963590665388Illumina DpnII expression Sequencing Primer (96% over 26bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA5681053.128016973623687Illumina RNA PCR Primer (100% over 45bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT4367642.4048462968426194Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT4357572.3993017001704566Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT3883522.138287196452603Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT3002611.653253367805638Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT2849961.5692034490364568Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA2285501.2584087084635653No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2275631.2529742328772449Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG2030111.117789587897164Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC1843871.0152448327607588Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT1747040.9619297090501803Illumina RNA PCR Primer (100% over 31bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC1416900.7801528326501972Illumina RNA PCR Primer (100% over 32bp)
AGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC1270060.6993019314247367Illumina RNA PCR Primer (100% over 39bp)
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1167310.6427272235732244Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA1131270.6228834039044312Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC1059120.5831572221868Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG995030.5478689202286158Illumina DpnII expression Sequencing Primer (95% over 24bp)
GGTGTCAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG977370.5381452283487355Illumina RNA PCR Primer (100% over 36bp)
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG974050.5363172183237522Illumina RNA PCR Primer (100% over 43bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG918550.5057586170024975Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT837730.4612586862135999Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG822960.45312624402414164Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT813580.44796156509813495Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA781970.43055692748075003No Hit
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG759780.4183389930065402Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA726790.40017451989684294No Hit
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT721320.39716270819905447Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG719110.3959458702004964Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG700460.3856770789456963Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT647510.3565225214689316Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC625190.3442330082889243Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG604370.33276940325273463Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC598400.3294822888403402Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG582100.32050742034418783Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC579540.31909787044540566Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAACCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG545570.30039380401508087Illumina RNA PCR Primer (100% over 36bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG522400.28763627622024346Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT515530.2838536169215584Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT509530.28054998434628764Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT491130.27041884444879055Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC487580.2684641951750887Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT483140.26601950706938826Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT480610.26462647533348244Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC468610.25801921018294083Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG460710.2536694272921676Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG437110.2406751391627692Illumina DpnII expression Sequencing Primer (96% over 26bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT428460.23591240220008713Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCG425560.23431564645537292Illumina RNA PCR Primer (100% over 43bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT408490.22491681177872752Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG387060.2131173374307187Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT383860.21135540005724093Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT381540.21007799546146955Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGCGGATCGTCGGACTGTAGAACTCTGAACGTGTA381230.20990730777841393Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAATGATCCTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG372460.20507849816422644Illumina RNA PCR Primer (100% over 33bp)
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT364560.20072871527345323Illumina DpnII expression Sequencing Primer (96% over 28bp)
ATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT364100.2004754367760158Illumina RNA PCR Primer (100% over 46bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG347680.19143449562835807Illumina DpnII expression Sequencing Primer (96% over 26bp)
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG345440.19020113946692363Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG344510.18968907641775667No Hit
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC336760.18542188434136525Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT335360.1846510367404687Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG332170.18289460542128308Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG329680.18152359790254569Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG326550.17980020290911278Illumina RNA PCR Primer (100% over 40bp)
ACAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG321370.17694806678579567Illumina RNA PCR Primer (100% over 40bp)
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT319120.1757092045700691Illumina DpnII expression Sequencing Primer (96% over 28bp)
CACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGT318920.17559908348422676Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT310420.17091893733592645No Hit
TGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT305740.16834210392721524Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATCAGTCTCATTGCTTTATAAGATCGTCGGACTGTAGAACTCTGAACGT302860.16675636029108526Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG302110.16634340621917643Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT294670.16224690182584064Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA293030.1613439089219333No Hit
AGCGACGCTCAGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT291720.16062261580966583Illumina RNA PCR Primer (100% over 31bp)
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC288940.15909193271645702No Hit
GGTCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC287690.15840367592994228Illumina RNA PCR Primer (100% over 39bp)
AGGTTTCAATTTCTATCGCCTATGATCGTCGGACTGTAGAACTCTGAACG282930.15578279408689413Illumina DpnII expression Sequencing Primer (96% over 26bp)
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT278490.15333810598119374Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT270910.1491645168277683Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGACGCTCAGACAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT269610.14844872976979298Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAACCCTTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG268690.1479421727749181Illumina RNA PCR Primer (100% over 34bp)
CCGCTAAGAGTCGTACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAG267730.14741359156287479Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT262960.14478720366553452Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC261860.14418153769340153No Hit
TGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCC260290.143317087169539Illumina RNA PCR Primer (100% over 42bp)
CCGCTAAGAGTCGTACGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT257860.14197911597655433Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA253770.1397271397710781No Hit
AACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG252660.13911596774465299Illumina DpnII expression Sequencing Primer (96% over 26bp)
TCAGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTG245340.1350855360028226Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGTCAGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG243170.133890722221433Illumina RNA PCR Primer (100% over 37bp)
AACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAAC242450.1334942863124005Illumina DpnII expression Sequencing Primer (100% over 26bp)
CAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT238740.13145154017002475Illumina RNA PCR Primer (100% over 31bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA232980.12828005289776478No Hit
ACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTGTA228880.12602257063799643Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAGATGTCCAGCCACAATTCTGATCGTCGGACTGTAGAACTCTGAACGT228220.12565917105471663Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT226010.12444233305615857Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT224470.12359440069517239Illumina RNA PCR Primer (100% over 38bp)
AGCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGT222330.12241610507665913Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG215780.11880963951532185Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACCTATCCTGAATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT212110.11678891759011457Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGTCAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT209770.11550050088575896Illumina RNA PCR Primer (100% over 38bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT209390.11529127082265847Illumina RNA PCR Primer (100% over 44bp)
ACAGGTTAAAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT208420.11475718355632301Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTCACCGACAGGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT206720.11382115432666297Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTG203540.11207022906176946Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCGATTTCAGATGGTGCTAGATCGTCGGACTGTAGAACTCTGAACGTG201760.11109015139777244Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT200380.11033031590546016Illumina RNA PCR Primer (100% over 44bp)
AACTATACAACCTCCTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT198330.10920157477557599Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA197570.10878311464937501No Hit
CGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT196400.10813890629719722Illumina RNA PCR Primer (100% over 44bp)
AGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGTA195830.10782506120254648Illumina DpnII expression Sequencing Primer (96% over 30bp)
TACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGT193330.106448547629517Illumina DpnII expression Sequencing Primer (96% over 28bp)
CCACGGGAACGTGGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA192220.1058373756030919Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT192190.10582085744021553Illumina RNA PCR Primer (100% over 39bp)
GCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC186320.10258880357074229Illumina RNA PCR Primer (100% over 41bp)
AGTTATCACAGTACTGTACGATCGTCGGACTGTAGAACTCTGAACGTGTA185690.10224192215033884Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC183850.10122880816058914Illumina RNA PCR Primer (100% over 39bp)
AGTCAGTTATCACAGTACTGTAGATCGTCGGACTGTAGAACTCTGAACGT182450.10045796055969261Illumina DpnII expression Sequencing Primer (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)