FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003058025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003058025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20578824
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA13278736.4526184781015665No Hit
AGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAA4912762.3872889918296596No Hit
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA4850832.357194949526756No Hit
ACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG4629572.2496766579081484Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAGTTCTTCAACTGGCAGCTTGATCGTCGGACTGTAGAACTCTGAACGT4568822.2201560205772695Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAG3738711.8167753414869579No Hit
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT3303971.605519343573763Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGA2609751.2681725641853976No Hit
CTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT2315971.125414163608183Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGA2062421.0022049850856396No Hit
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT2051010.9966604505680208Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAAC1883230.9151300385289267No Hit
CAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1829120.8888360190067227Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAAC1783870.8668473961388659No Hit
GAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG1680550.8166404455376071No Hit
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT1671540.8122621584207145Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAA1551230.7537991480951487No Hit
TCATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACG1476470.7174705415625305Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACT1441830.700637704078717No Hit
CAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGA1426800.6933340797316697No Hit
TCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACGTG1281080.6225234250509164Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACGT1277350.6207108822156212Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAG1207280.5866613174785886No Hit
GCAGGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAG1162780.5650371469234589No Hit
AAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGA1130070.549142166724396No Hit
GAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAG1120740.5446083799540732No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC1114620.5416344490822216No Hit
AGAGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTA955300.464215059130687No Hit
AACCAGGAATCCTAACCGCTAGACCATGTGGGAGATCGTCGGACTGTAGA953210.46319945201922136No Hit
GGAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT945190.4593022419551282Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATAAGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGT936990.45531756333598067No Hit
GAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG895590.4351997956734554No Hit
AGCCTATCCTGGATTACTTGAAGATCCGTCGGACTGTAGAACTCTGAACG880070.4276580624820932Illumina DpnII expression Sequencing Primer (95% over 24bp)
GGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGAA860130.4179684903277272No Hit
CAGGCGAACGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAG783730.3808429480712795No Hit
GCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTAGAA769950.37414674424544375No Hit
AGCTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC703810.3420069096271002Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTG702810.341520973210131Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAG631090.3066696133850992No Hit
GCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAGAAC588080.2857694880912534No Hit
CAGCTGCTTTTGGGATTCCGTTGGATCGTCGGACTGTAGAACTCTGAACG572840.27836381709664265Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAACTC566300.275185792929664Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG559180.27172592564084325Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA545640.2651463465550801No Hit
AGAGCGCCGAATCCTAACCACTAGACCACCAGGGAGATCGTCGGACTGTA521760.2535421849178554No Hit
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT508480.24708894930050426Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTG506420.24608792028154766Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT499060.2425114282526543Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTGTACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT494890.24048507339389266Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAACGTGATAACCGCTACACTACGGAAACGATCGTCGGACTGTAGA489080.23766178281130157No Hit
AGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGTGT470070.22842413152471686Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAACT446890.21716012537937057No Hit
AGCTTCCAGTCGGGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC445070.2162757211004866Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGACGCTCAGACAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC439620.21362736762800438Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG438880.21326777467944716Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAACGTGATGACCACTACACTACGGAAACGATCGTCGGACTGTAGA423850.20596415033239993No Hit
AGCTTCCAGTCAAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC421010.20458409090820742Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAACG416210.2022515961067552Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGA412020.2002155225196542No Hit
CAGGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAA407600.1980676835566503No Hit
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT400980.1948507844763141Illumina RNA PCR Primer (100% over 31bp)
AGGCGAAAGTGATAACCACTACACTACGGAAACGATCGTCGGACTGTAGA395340.19211010308460777No Hit
CAGGCGAGAATTCTACCACTGAACCACCCATGCGATCGTCGGACTGTAGA394050.19148324510671746No Hit
AGAGCGCCGAATCCTAACCACTAGACCACAGGGAGATCGTCGGACTGTAG388810.1889369382817988No Hit
GGCGAGAATTCTACCACTGAACCACAATGCGATCGTCGGACTGTAGAACT385090.18712925481067336No Hit
AACGCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGA383460.18633717845101352No Hit
ACAGGCCATCTGTGTTATATTGATCGTCGGACTGTAGAACTCTGAACGTG379510.18441772960398514Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGAGCGCCGAATCCTAACCACTAGACCAGGGAGATCGTCGGACTGTAGAA371650.18059826936660714No Hit
AGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGAA361350.17559312427182427No Hit
AACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGTG355700.17284758351594823Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG350500.17032071414770836Illumina DpnII expression Sequencing Primer (96% over 29bp)
CAATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAGA343920.16712325252405094No Hit
ATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGTG337790.16414446228802967Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCAATTGAGATAACTCACTACCTTCGGACCAGCCGATCGTCGGACTGTAG337360.1639355096287329No Hit
AAGCCAGTCAAATTTAGCAGTGGGGGGGATCGTCGGACTGTAGAACTCTG333260.16194317031915914Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACG329110.15992653418873692Illumina DpnII expression Sequencing Primer (96% over 26bp)
CGCCAATATTTACGTGCTGCTAGATCGTCGGACTGTAGAACTCTGAACGT326200.1585124592153565Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGCGTACCAAAAGTAATAATGGATCGTCGGACTGTAGAACTCTGAACGTG323220.15706436869278828Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGCGAACGTGATAACCACTACACTACAGAAGCGATCGTCGGACTGTAGA318240.1546444053362816No Hit
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT309330.15031471186108594Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGACGCTCAGACAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA296240.14395380416295897Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAATCAGCTAACTACACTGCCTGATCGTCGGACTGTAGAACTCTGAACG293760.14274868184887532Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACAAACCCTTGTGTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC293510.14262719774463303Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCATAGCCCTGTACAATGCTGCTGATCGTCGGACTGTAGAACTCTGAACG288840.1403578746773868Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCTGAGAGTGTAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAAC288040.13996912554381144Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAGGCCGGGACAAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGT280730.13641693033576652Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCACACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT277180.1346918560555258Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGGCGAGAATTCTACCACTGAACCACAAATGCGATCGTCGGACTGTAGA275730.13398724825092045No Hit
ACTAGACTGTGAGCTCCTCGAGATCGTCGGACTGTAGAACTCTGAACGTG270430.13141178524098363Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGTCAGAGCGGTCAAGTTAAGTTGAAATCTCCTGATCGTCGGACTGTAGA262320.12747084089936334No Hit
GCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGTG262160.12739309107264826Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACAAACCCTTGTGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT261890.1272618882400666Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAACG251620.1222713212377928Illumina DpnII expression Sequencing Primer (96% over 26bp)
GTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGAA245820.11945289001937137No Hit
TCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGTGT244050.11859278256133587Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACAAGTTAGGGTCTCAGGGAGATCGTCGGACTGTAGAACTCTGAACGT243380.1182672051619665Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACCAGGAATCCTAACCGCTAGACCATATGGGAGATCGTCGGACTGTAGA243250.11820403342776051No Hit
AACAATACAACTTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT238220.11575977325040537Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACG237190.11525925874092707Illumina DpnII expression Sequencing Primer (96% over 26bp)
AAGCCAAGCTCAGACGGATCCGAGATCGTCGGACTGTAGAACTCTGAACG237090.11521066509923017Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTGGACTGTAGAAC232750.1131017010495838No Hit
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC232030.11275182682936595Illumina RNA PCR Primer (100% over 32bp)
AACTCACCGACAGCGTTGAATGTTGATCGTCGGACTGTAGAACTCTGAAC227710.11065258150805897Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCTCAGAACCCAGAGGTCTCAGATCGTCGGACTGTAGAACTCTGAACGT224330.10901011641870303Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT223490.1086019298284489Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA220680.1072364484967654No Hit
GAGGCCGGGACGAGTGCAATAGATCGTCGGACTGTAGAACTCTGAACGTG219310.10657071560551759Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGGCCTCGATCAGAAGGACTTGGGCCCCCCACGATCGTCGGACTGTAGAA218940.10639091913123898No Hit
GAGCGCCGAATCCTAACCACTAGACACAGGGAGATCGTCGGACTGTAGAA216430.10517121872464627No Hit
AGCGACGCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTG216010.10496712542951919No Hit
AAGAGACCGGTTCACTGTGAGATCGTCGGACTGTAGAACTCTGAACGTGT213280.10364051901119326Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCCACCGACAGCAATGAATGTTGATCGTCGGACTGTAGAACTCTGAACGT211940.10298936421245453Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACATGCGATCGTCGGACTGTAGAACT210750.10241109987626114No Hit
AGTCTGATGCTCTACCGACTGAGCTATCCGGGCGATCGTCGGACTGTAGA209530.10181825744755872No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA209360.10173564825667394Illumina RNA PCR Primer (100% over 45bp)
CAACAAAATCACTGATGCTGGAGATCGTCGGACTGTAGAACTCTGAACGT206100.10015149553735433Illumina DpnII expression Sequencing Primer (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)