FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003090003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003090003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8336
Sequences flagged as poor quality0
Sequence length45-575
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGTGCGTACACTGACGACATGGTTCTACATAGGGCTCCCTGGACCCA1661.9913627639155471No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACACCATTTATGAACTCTCAA1531.8354126679462572No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGGGCTAATTGCGGGACT1361.6314779270633395No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGCCTGTGGATCCCTAGC1311.5714971209213051No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAGCTCTGTCAAACATAAA1271.5235124760076775No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGGAACTGCTGTCTGCAT1271.5235124760076775No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTAGGAGCTGGAGGCAGAG1211.4515355086372361No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATCGCATCACCAATGCCTT1191.4275431861804224No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAATCCCGTGGCCTAAAGAA1191.4275431861804224No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAACATGCTACTTACCAGGT1181.4155470249520155No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGGAAGCTAGAAGAAACA1181.4155470249520155No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAGGGAAAGGGCGTCATCAG1131.3555662188099808No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGTAGTGAAAGTAGGAGGA1101.3195777351247602No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATCCCACAGCATGACCTAC1101.3195777351247602No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATTCATGCGCCTTTCCATT1071.2835892514395395No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAGGTGAGTCACAGGTTCAG1071.2835892514395395No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGCCCAGGCCTTTCTCCAC1051.2595969289827254No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATAATCAGTGTGATTCGTG1041.2476007677543186No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGCCTTACTGAGGCTTGCT1041.2476007677543186No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGGAGAGAGTGAAGAAAC1021.2236084452975047No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAGTTGCTGTCCTGTCACT1011.211612284069098No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAATCTTCCCCTTGACTTT1011.211612284069098No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACACCTGCGCATGTACACTCA1011.211612284069098No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGTGCATCAGTGGCTTTT1011.211612284069098No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCACTATGGCTTAGCGTCT971.1636276391554703No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTAGCCCACCCTGGATTAA961.1516314779270633No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTAAGGACCCATTAGAACCA931.1156429942418424No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCTAACTCCTGGTGTCTCT921.1036468330134357No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAGTGTGTCACTCGTAATTA911.0916506717850287No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCCCTTTTGCCTGCAGGAG911.0916506717850287No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGGCTGACCTAAAGCCACC911.0916506717850287No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATCATGAGGGGAGGCAGCT901.0796545105566218No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGGAGGCAAAAGAAAGGC881.055662188099808No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAGGCCTAATGATTATTTTT881.055662188099808No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGAGCTCTGTGCCCTATCT851.0196737044145874No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTCCAGAGGAGGAGTATGT851.0196737044145874No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATTCTAGCAGTATCGACAA851.0196737044145874No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACACCCGAATTTTGCTAATCC841.0076775431861804No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCCCAGTCAAGCATATTGA841.0076775431861804No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCTTTTGCCTCCATCCTCA841.0076775431861804No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCTCCCCACCAGACCATGA830.9956813819577736No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACACCATGGACACTGGATTAA810.9716890595009597No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATTTCTGTCCTTTGCAAGC790.9476967370441458No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGTGGTTGTCATCAAGAAA770.923704414587332No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCATGAGAAAAGGTGGGCC770.923704414587332No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAGAAGGCGTACATTTGTC770.923704414587332No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAGCAAGGGATTGTGATTG760.9117082533589251No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAGTCTGCATGGAGCAGGA750.8997120921305182No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTATGGTGCTCTTCCCTCCT740.8877159309021113No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTGTGCCTGCCTTAAGTAA730.8757197696737045No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTATTTCCTCTGCCGTGTTT730.8757197696737045No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGGCGTTTCTAATAATGCC720.8637236084452975No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAGTGTCTGCCACATAGAA720.8637236084452975No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACACGATGAACAATGACATCT720.8637236084452975No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTACAAAGCCTCAAATGTAA710.8517274472168906No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAAAGCCAAAGGAGGATGG700.8397312859884838No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAGCCATGAAACGAACCTT700.8397312859884838No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAACATATGGCCAATAGCGT680.8157389635316697No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTACGATACACGTCTGCAG670.8037428023032629No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTACAAACCAGGATTCTAGC670.8037428023032629No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTAAACAGCAGATGGTCATA660.791746641074856No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATCATGAAAATGGTCAGAG640.7677543186180422No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAACAGCTTTTCCTCCATGA630.7557581573896353No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGAATGTCTGTGGTTTGGT620.7437619961612284No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTCCAAGCAAGGCAAACAC610.7317658349328215No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGGATCCTGGCTCCTTATC610.7317658349328215No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATCTGCGACATCCCTGGGA590.7077735124760077No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTGGTTAAGCATTTATGTG590.7077735124760077No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTACCTTATTTCCCCCCATT560.6717850287907869No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTATTCAGTTGCCTGAAGAG560.6717850287907869No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATAACTTGGGCTTTCTGAC550.6597888675623801No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTGAGTAGACACAGCTTGA550.6597888675623801No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTAGTGCCTTCCCATTGCCT510.6118042226487523No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAGCCCTACTCAGGTTAATG500.5998080614203455No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTGTGTTACTAATGACTGT480.5758157389635317No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATTTCCCTGTCATACCCCC480.5758157389635317No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTAGCAGAACACTCTTTTCT470.5638195777351248No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTTTCCCAAGGTCTCAGTG460.5518234165067178No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTAGGGTTTAGTGACTATTG440.527831094049904No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACACCAGAGAGAGTGGCACAG430.5158349328214972No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCTAGATTGACGCCTTTTG420.5038387715930902No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAACAAACAATAAAACCTTT390.4678502879078695No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGAAACTTGTTTTCCAAA380.45585412667946257No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCTCTGTGCCACTCTCTCT370.44385796545105566No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTACTGTCGCAAACAAGACT370.44385796545105566No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTAGATTCCATAGAGATATT360.43186180422264875No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTAGGAAATGCTCTTATTAT270.32389635316698656No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAACAGGGATATTACAGCAC210.2519193857965451No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGGCGTTTCTAATGTGGCA200.23992322456813817No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAACAGGGATATTACAGTGC190.22792706333973128No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGGTTGCCACCTTGTTAC180.21593090211132437No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGCGCAAAGATTTAAAAA160.19193857965451055No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCCCAGAGGAGGAGTATGT160.19193857965451055No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTAGGTGCTGTAATATCCCT140.16794625719769674No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCCCAAGCAAGGCAAACAC130.15595009596928983No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTACCTTATTTCCCCCATTT120.14395393474088292No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGGTTAAGCATTTATGTG120.14395393474088292No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGCAGAACACTCTTTTCT110.131957773512476No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCCAAAGCCTCAAATGTAA110.131957773512476No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGTGCCTGCCTTAAGTAA100.11996161228406908No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGGAGAGACCAAGTCTCTGCTACCGTAACGCACTCGT100.11996161228406908No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAATCTTCCCCTTGACTGG100.11996161228406908No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGAGTAGACACAGCTTGA100.11996161228406908No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATTTGGGCTTTGTAGACCA90.10796545105566219No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)