FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003090004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003090004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7943
Sequences flagged as poor quality0
Sequence length47-556
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCTCGACAACACTGACGACATGGTTCTACATAGGGCTCCCTGGACCCA1622.039531663099585No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCGGAACTGCTGTCTGCAT1481.8632758403625833No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAATCCCGTGGCCTAAAGAA1421.7877376306181543No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATGGGCTAATTGCGGGACT1361.7121994208737252No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATCGCATCACCAATGCCTT1301.6366612111292964No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAGGTGAGTCACAGGTTCAG1201.5107641948885813No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAAGCTCTGTCAAACATAAA1181.485584791640438No Hit
ACGCTCGACAACACTGACGACATGGTTCTACACCTGCGCATGTACACTCA1161.4604053883922952No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATGCCTGTGGATCCCTAGC1151.4478156867682235No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAAATCTTCCCCTTGACTTT1141.435225985144152No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTTAGGAGCTGGAGGCAGAG1061.33450837215158No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATGGAGAGAGTGAAGAAAC1061.33450837215158No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTGAGCTCTGTGCCCTATCT1011.2715598640312225No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAAGTTGCTGTCCTGTCACT981.233790759159008No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATGGAAGCTAGAAGAAACA971.2212010575349364No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAGTGTGTCACTCGTAATTA961.2086113559108649No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTTAGCCCACCCTGGATTAA941.183431952662722No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAGGGAAAGGGCGTCATCAG921.1582525494145788No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTGTAGTGAAAGTAGGAGGA921.1582525494145788No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATTCATGCGCCTTTCCATT921.1582525494145788No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTGCCTTACTGAGGCTTGCT911.1456628477905073No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCTAACTCCTGGTGTCTCT911.1456628477905073No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCGTGCATCAGTGGCTTTT891.1204834445423644No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCCCAGTCAAGCATATTGA891.1204834445423644No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATCATGAGGGGAGGCAGCT881.1078937429182927No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATCCCACAGCATGACCTAC881.1078937429182927No Hit
ACGCTCGACAACACTGACGACATGGTTCTACACGATGAACAATGACATCT871.0953040412942214No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCATGAGAAAAGGTGGGCC861.08271433967015No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATAATCAGTGTGATTCGTG851.0701246380460783No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCCCTTTTGCCTGCAGGAG851.0701246380460783No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAGCCCTACTCAGGTTAATG841.0575349364220068No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAAGTGTCTGCCACATAGAA841.0575349364220068No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTGGCTGACCTAAAGCCACC841.0575349364220068No Hit
ACGCTCGACAACACTGACGACATGGTTCTACACCATTTATGAACTCTCAA841.0575349364220068No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATTCTAGCAGTATCGACAA831.0449452347979353No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTGCCCAGGCCTTTCTCCAC831.0449452347979353No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAACAGGGATATTACAGTGC821.0323555331738639No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATTTCTGTCCTTTGCAAGC811.0197658315497924No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATAACTTGGGCTTTCTGAC801.0071761299257207No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCTTTTGCCTCCATCCTCA780.9819967266775778No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTTCCAGAGGAGGAGTATGT760.9568173234294347No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAACATATGGCCAATAGCGT730.9190482185572202No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAACATGCTACTTACCAGGT720.9064585169331486No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTATGGTGCTCTTCCCTCCT720.9064585169331486No Hit
ACGCTCGACAACACTGACGACATGGTTCTACACCATGGACACTGGATTAA720.9064585169331486No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAAGAAGGCGTACATTTGTC720.9064585169331486No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTGAATGTCTGTGGTTTGGT710.8938688153090771No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCTCCCCACCAGACCATGA710.8938688153090771No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATGGAGGCAAAAGAAAGGC700.8812791136850056No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTAAACAGCAGATGGTCATA690.8686894120609341No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTGGCGTTTCTAATAATGCC670.8435100088127913No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTAAGGACCCATTAGAACCA670.8435100088127913No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAGGCCTAATGATTATTTTT650.8183306055646482No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTGTGGTTGTCATCAAGAAA640.8057409039405766No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTTGTGCCTGCCTTAAGTAA640.8057409039405766No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAAGCCATGAAACGAACCTT640.8057409039405766No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTTCCAAGCAAGGCAAACAC630.7931512023165052No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTATTTCCTCTGCCGTGTTT630.7931512023165052No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCACTATGGCTTAGCGTCT610.7679717990683621No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTAGCAGAACACTCTTTTCT610.7679717990683621No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAACAGCTTTTCCTCCATGA610.7679717990683621No Hit
ACGCTCGACAACACTGACGACATGGTTCTACACCCGAATTTTGCTAATCC610.7679717990683621No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATCATGAAAATGGTCAGAG590.742792395820219No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTAGGGTTTAGTGACTATTG580.7302026941961476No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAAAAGCCAAAGGAGGATGG570.717612992572076No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTAGGTGCACTGTAATATCC570.717612992572076No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATCTGCGACATCCCTGGGA560.7050232909480045No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTTGAGTAGACACAGCTTGA560.7050232909480045No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAAGTCTGCATGGAGCAGGA550.692433589323933No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTTGGTTAAGCATTTATGTG540.6798438876998615No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTAGTGCCTTCCCATTGCCT540.6798438876998615No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATTTCCCTGTCATACCCCC530.66725418607579No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTATTCAGTTGCCTGAAGAG520.6546644844517185No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAAGCAAGGGATTGTGATTG510.642074782827647No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTACAAACCAGGATTCTAGC510.642074782827647No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTGGATCCTGGCTCCTTATC500.6294850812035755No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTACAAAGCCTCAAATGTAA500.6294850812035755No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTTACGATACACGTCTGCAG470.591715976331361No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATGAAACTTGTTTTCCAAA460.5791262747072894No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTACTGTCGCAAACAAGACT440.5539468714591463No Hit
ACGCTCGACAACACTGACGACATGGTTCTACAACAAACAATAAAACCTTT420.5287674682110034No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCTAGATTGACGCCTTTTG420.5287674682110034No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTTTTCCCAAGGTCTCAGTG410.5161777665869319No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTTGTGTTACTAATGACTGT400.5035880649628603No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTAGATTCCATAGAGATATT340.4280498552184313No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTACCTTATTTCCCCCCATT320.4028704519702883No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATGCGCAAAGATTTAAAAA310.3902807503462168No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCGGTGCACTGTAATATCC290.3651013470980738No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATGGTTGCCACCTTGTTAC240.3021528389777162No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTACCTTATTTCCCCCATTT230.2895631373536447No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTAGGAAATGCTCTTATTAT190.23920433085735868No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCGGCATCTTTTGGTTTGC190.23920433085735868No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTACCTGATTTCTTTAGGCC190.23920433085735868No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCGGTTAAGCATTTATGTG170.21402492760921565No Hit
ACGCTCGACAACACTGACGACATGGTTCTACACCATTTATGAAAGCCACT150.18884552436107266No Hit
ACGCTCGACAACACTGACGACATGGTTCTACACGAGACCAAGTCTCTGCTACCGTATGTCGAGCGT140.17625582273700113No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCCCAGAGGAGGAGTATGT130.16366612111292964No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCGATTCCATAGAGATATT130.16366612111292964No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTACCTGATTCCCATTAACC120.1510764194888581No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCAGGAGCTGGAGGCAGAG120.1510764194888581No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCGTGTAGAACCATGTCGTCAGTGTTGTCGAGCGT120.1510764194888581No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCTTCCCAAGGTCTCAGTG100.1258970162407151No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCGGGTTTAGTGACTATTG100.1258970162407151No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCCAAAGCCTCAAATGTAA100.1258970162407151No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCGTGCCTGCCTTAAGTAA90.11330731461664358No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCCCAAGCAAGGCAAACAC90.11330731461664358No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCTTTCCTCTGCCGTGTTT80.10071761299257208No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTGTAGAACCATGTCGTCAGTGTTGTCGAGCGT80.10071761299257208No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATGGAGAGACCAAGTCTCTGCTACCGTATGTCGAGCGT80.10071761299257208No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTGCTTTGGAAACAAGTTTC80.10071761299257208No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCGCAGAACACTCTTTTCT80.10071761299257208No Hit
ACGCTCGACAACACTGACGACATGGTTCTACATCATGAAATTCCAGTTGA80.10071761299257208No Hit
ACGCTCGACATACGGTAGCAGAGACTTGGTCTCCCAGTCAAGGGAAGATT80.10071761299257208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)