FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003090005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003090005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9778
Sequences flagged as poor quality0
Sequence length48-597
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGACTACAGACACTGACGACATGGTTCTACATAGGGCTCCCTGGACCCA1651.6874616485988956No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCGGAACTGCTGTCTGCAT1631.6670075680098178No Hit
ACGACTACAGACACTGACGACATGGTTCTACAATCCCGTGGCCTAAAGAA1581.615872366537124No Hit
ACGACTACAGACACTGACGACATGGTTCTACATGGGCTAATTGCGGGACT1521.5545101247698916No Hit
ACGACTACAGACACTGACGACATGGTTCTACATCGCATCACCAATGCCTT1451.4829208427081202No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTTAGGAGCTGGAGGCAGAG1431.4624667621190428No Hit
ACGACTACAGACACTGACGACATGGTTCTACAGTGTGTCACTCGTAATTA1381.411331560646349No Hit
ACGACTACAGACACTGACGACATGGTTCTACAGGTGAGTCACAGGTTCAG1351.3806504397627326No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTGCCCAGGCCTTTCTCCAC1331.3601963591736552No Hit
ACGACTACAGACACTGACGACATGGTTCTACATGCCTGTGGATCCCTAGC1331.3601963591736552No Hit
ACGACTACAGACACTGACGACATGGTTCTACACCTGCGCATGTACACTCA1281.3090611577009614No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTGCCTTACTGAGGCTTGCT1281.3090611577009614No Hit
ACGACTACAGACACTGACGACATGGTTCTACATGGAAGCTAGAAGAAACA1251.278380036817345No Hit
ACGACTACAGACACTGACGACATGGTTCTACAAATCTTCCCCTTGACTTT1241.2681529965228062No Hit
ACGACTACAGACACTGACGACATGGTTCTACAGGGAAAGGGCGTCATCAG1241.2681529965228062No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTGTAGTGAAAGTAGGAGGA1231.2579259562282676No Hit
ACGACTACAGACACTGACGACATGGTTCTACATTCATGCGCCTTTCCATT1231.2579259562282676No Hit
ACGACTACAGACACTGACGACATGGTTCTACAACATGCTACTTACCAGGT1231.2579259562282676No Hit
ACGACTACAGACACTGACGACATGGTTCTACAAGCTCTGTCAAACATAAA1211.23747187563919No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTGGCTGACCTAAAGCCACC1161.1863366741664962No Hit
ACGACTACAGACACTGACGACATGGTTCTACATGGAGAGAGTGAAGAAAC1151.1761096338719574No Hit
ACGACTACAGACACTGACGACATGGTTCTACAAGTTGCTGTCCTGTCACT1151.1761096338719574No Hit
ACGACTACAGACACTGACGACATGGTTCTACACGATGAACAATGACATCT1141.1658825935774186No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTTAGCCCACCCTGGATTAA1141.1658825935774186No Hit
ACGACTACAGACACTGACGACATGGTTCTACAAGCAAGGGATTGTGATTG1111.1352014726938024No Hit
ACGACTACAGACACTGACGACATGGTTCTACATCCCACAGCATGACCTAC1111.1352014726938024No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTGAGCTCTGTGCCCTATCT1081.1045203518101863No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTATTTCCTCTGCCGTGTTT1071.0942933115156472No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCTAACTCCTGGTGTCTCT1061.0840662712211087No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCACTATGGCTTAGCGTCT1061.0840662712211087No Hit
ACGACTACAGACACTGACGACATGGTTCTACATAACTTGGGCTTTCTGAC1031.0533851503374925No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCCCTTTTGCCTGCAGGAG1031.0533851503374925No Hit
ACGACTACAGACACTGACGACATGGTTCTACAAGAAGGCGTACATTTGTC1011.0329310697484149No Hit
ACGACTACAGACACTGACGACATGGTTCTACACCATGGACACTGGATTAA1011.0329310697484149No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTTCCAGAGGAGGAGTATGT1011.0329310697484149No Hit
ACGACTACAGACACTGACGACATGGTTCTACACCATTTATGAACTCTCAA1001.022704029453876No Hit
ACGACTACAGACACTGACGACATGGTTCTACAAGTGTCTGCCACATAGAA1001.022704029453876No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCTTTTGCCTCCATCCTCA970.9920229085702597No Hit
ACGACTACAGACACTGACGACATGGTTCTACAACATATGGCCAATAGCGT970.9920229085702597No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTGGCGTTTCTAATAATGCC950.9715688279811823No Hit
ACGACTACAGACACTGACGACATGGTTCTACATTCTAGCAGTATCGACAA930.9511147473921048No Hit
ACGACTACAGACACTGACGACATGGTTCTACATCATGAGGGGAGGCAGCT920.9408877070975659No Hit
ACGACTACAGACACTGACGACATGGTTCTACATGGAGGCAAAAGAAAGGC920.9408877070975659No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTAAGGACCCATTAGAACCA920.9408877070975659No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTACAAAGCCTCAAATGTAA900.9204336265084885No Hit
ACGACTACAGACACTGACGACATGGTTCTACAAGTCTGCATGGAGCAGGA900.9204336265084885No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCTCCCCACCAGACCATGA890.9102065862139497No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTTGTGCCTGCCTTAAGTAA890.9102065862139497No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTGTGGTTGTCATCAAGAAA890.9102065862139497No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCGTGCATCAGTGGCTTTT880.899979545919411No Hit
ACGACTACAGACACTGACGACATGGTTCTACAGGCCTAATGATTATTTTT870.8897525056248721No Hit
ACGACTACAGACACTGACGACATGGTTCTACATTTCTGTCCTTTGCAAGC870.8897525056248721No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCCCAGTCAAGCATATTGA870.8897525056248721No Hit
ACGACTACAGACACTGACGACATGGTTCTACACCCGAATTTTGCTAATCC860.8795254653303334No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTTCCAAGCAAGGCAAACAC850.8692984250357947No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTGAATGTCTGTGGTTTGGT830.8488443444467172No Hit
ACGACTACAGACACTGACGACATGGTTCTACATAATCAGTGTGATTCGTG820.8386173041521783No Hit
ACGACTACAGACACTGACGACATGGTTCTACATGCGCAAAGATTTAAAAA810.8283902638576396No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTTGGTTAAGCATTTATGTG800.8181632235631009No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTAGGGTTTAGTGACTATTG790.807936183268562No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCATGAGAAAAGGTGGGCC790.807936183268562No Hit
ACGACTACAGACACTGACGACATGGTTCTACATCATGAAAATGGTCAGAG790.807936183268562No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTATTCAGTTGCCTGAAGAG780.7977091429740233No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTATGGTGCTCTTCCCTCCT760.7772550623849458No Hit
ACGACTACAGACACTGACGACATGGTTCTACAAGCCATGAAACGAACCTT750.767028022090407No Hit
ACGACTACAGACACTGACGACATGGTTCTACAACAGCTTTTCCTCCATGA750.767028022090407No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTTACGATACACGTCTGCAG740.7568009817958683No Hit
ACGACTACAGACACTGACGACATGGTTCTACAGCCCTACTCAGGTTAATG720.7363469012067908No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTTTTCCCAAGGTCTCAGTG700.7158928206177132No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTAGTGCCTTCCCATTGCCT690.7056657803231745No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTAAACAGCAGATGGTCATA660.6749846594395582No Hit
ACGACTACAGACACTGACGACATGGTTCTACATCTGCGACATCCCTGGGA650.6647576191450194No Hit
ACGACTACAGACACTGACGACATGGTTCTACAAAAGCCAAAGGAGGATGG610.6238494579668643No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTGGATCCTGGCTCCTTATC600.6136224176723257No Hit
ACGACTACAGACACTGACGACATGGTTCTACATTTCCCTGTCATACCCCC590.6033953773777869No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTACCTTATTTCCCCCCATT570.5829412967887093No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTACAAACCAGGATTCTAGC560.5727142564941705No Hit
ACGACTACAGACACTGACGACATGGTTCTACAACAGGGATATTACAGTGC560.5727142564941705No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCTAGATTGACGCCTTTTG550.5624872161996318No Hit
ACGACTACAGACACTGACGACATGGTTCTACATGAAACTTGTTTTCCAAA550.5624872161996318No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTAGCAGAACACTCTTTTCT540.5522601759050931No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTACTGTCGCAAACAAGACT530.5420331356105543No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTTGAGTAGACACAGCTTGA510.5215790550214767No Hit
ACGACTACAGACACTGACGACATGGTTCTACAACAAACAATAAAACCTTT480.4908979341378605No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTTGTGTTACTAATGACTGT430.4397627326651667No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTAGGAAATGCTCTTATTAT400.40908161178155045No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTAGGTGCACTGTAATATCC380.3886275311924729No Hit
ACGACTACAGACACTGACGACATGGTTCTACAACAGGGATATTACCTACC350.3579464103088566No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCGGCATCTTTTGGTTTGC340.34771937001431785No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTAGATTCCATAGAGATATT290.29658416854162406No Hit
ACGACTACAGACACTGACGACATGGTTCTACATGGTTGCCACCTTGTTAC260.26590304765800776No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCCCAGAGGAGGAGTATGT210.21476784618531397No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCAGGAGCTGGAGGCAGAG200.20454080589077522No Hit
ACGACTACAGACACTGACGACATGGTTCTACATGGAGAGACCAAGTCTCTGCTACCGTACTGTAGTCGT190.19431376559623645No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCCAAAGCCTCAAATGTAA180.1840867253016977No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCGGTGCACTGTAATATCC170.17385968500715893No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCCAGAGGAGGAGTATGTG160.16363264471262018No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCAGGACCCATTAGAACCA150.15340560441808143No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCGAGTAGACACAGCTTGA150.15340560441808143No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTACCTGATTTCTTTAGGCC140.14317856412354263No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCGGTTAAGCATTTATGTG140.14317856412354263No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCCCAAGCAAGGCAAACAC140.14317856412354263No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTAGGTGCACTGTAATAATC140.14317856412354263No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCAGCCCACCCTGGATTAA130.13295152382900388No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCTGGTGCTCTTCCCTCCT130.13295152382900388No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCGTGTTACTAATGACTGT130.13295152382900388No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTGGCGTTTCTATGTGGCAG110.11249744323992637No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCCTGTCGCAAACAAGACT110.11249744323992637No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCTTTCCTCTGCCGTGTTT110.11249744323992637No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCGATTCCATAGAGATATT100.10227040294538761No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCCCTTATTTCCCCCCATT100.10227040294538761No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTGGGTTTAGTGACTATTGT100.10227040294538761No Hit
ACGACTACAGTACGGTAGCAGAGACTTGGTCTCCCAGTCAAGGGAAGATT100.10227040294538761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)