FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003090006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003090006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7377
Sequences flagged as poor quality0
Sequence length49-558
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCGAGTATACACTGACGACATGGTTCTACATGGGCTAATTGCGGGACT1381.8706791378609189No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCGGAACTGCTGTCTGCAT1261.7080113867425781No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATAGGGCTCCCTGGACCCA1261.7080113867425781No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCTCCCCACCAGACCATGA1061.4368984682120103No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAGGGAAAGGGCGTCATCAG1021.3826758845058966No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAGGTGAGTCACAGGTTCAG1021.3826758845058966No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATGGAGAGAGTGAAGAAAC1011.3691202385793684No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTTAGGAGCTGGAGGCAGAG1011.3691202385793684No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAGTGTGTCACTCGTAATTA1011.3691202385793684No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTGAGCTCTGTGCCCTATCT1001.35556459265284No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATGCCTGTGGATCCCTAGC1001.35556459265284No Hit
ACGCGAGTATACACTGACGACATGGTTCTACACCTGCGCATGTACACTCA991.3420089467263114No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATCCCACAGCATGACCTAC981.3284533007997832No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATCGCATCACCAATGCCTT971.3148976548732547No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAATCCCGTGGCCTAAAGAA961.3013420089467262No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAACATGCTACTTACCAGGT921.2471194252406126No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTTAGCCCACCCTGGATTAA911.2335637793140843No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTGCCTTACTGAGGCTTGCT901.2200081333875559No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATTCATGCGCCTTTCCATT891.2064524874610274No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATCATGAGGGGAGGCAGCT881.1928968415344992No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCTAACTCCTGGTGTCTCT871.1793411956079707No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTGGCTGACCTAAAGCCACC871.1793411956079707No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATTCTAGCAGTATCGACAA841.1386742578283855No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCGTGCATCAGTGGCTTTT841.1386742578283855No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATGGAAGCTAGAAGAAACA791.0708960281957436No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATAACTTGGGCTTTCTGAC791.0708960281957436No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAAGAAGGCGTACATTTGTC781.0573403822692151No Hit
ACGCGAGTATACACTGACGACATGGTTCTACACGATGAACAATGACATCT781.0573403822692151No Hit
ACGCGAGTATACACTGACGACATGGTTCTACACCATTTATGAACTCTCAA781.0573403822692151No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAGGCCTAATGATTATTTTT781.0573403822692151No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCCCTTTTGCCTGCAGGAG771.0437847363426866No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAAATCTTCCCCTTGACTTT761.0302290904161582No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTGTGGTTGTCATCAAGAAA761.0302290904161582No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTAAGGACCCATTAGAACCA751.01667344448963No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTGAATGTCTGTGGTTTGGT741.0031177985631015No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTGTAGTGAAAGTAGGAGGA741.0031177985631015No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTTCCAGAGGAGGAGTATGT730.9895621526365731No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAAGTTGCTGTCCTGTCACT720.9760065067100446No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATGGAGGCAAAAGAAAGGC710.9624508607835163No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATTTCTGTCCTTTGCAAGC710.9624508607835163No Hit
ACGCGAGTATACACTGACGACATGGTTCTACACCATGGACACTGGATTAA710.9624508607835163No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATAATCAGTGTGATTCGTG710.9624508607835163No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATCATGAAAATGGTCAGAG700.9488952148569879No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTGGCGTTTCTAATAATGCC690.9353395689304594No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAACAGCTTTTCCTCCATGA680.9217839230039311No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTTCCAAGCAAGGCAAACAC680.9217839230039311No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCTTTTGCCTCCATCCTCA680.9217839230039311No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTGCCCAGGCCTTTCTCCAC680.9217839230039311No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAAGCCATGAAACGAACCTT670.9082282770774027No Hit
ACGCGAGTATACACTGACGACATGGTTCTACACCCGAATTTTGCTAATCC660.8946726311508743No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTGGATCCTGGCTCCTTATC650.8811169852243459No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAAGCTCTGTCAAACATAAA650.8811169852243459No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTATTTCCTCTGCCGTGTTT650.8811169852243459No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTAAACAGCAGATGGTCATA630.8540056933712891No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCACTATGGCTTAGCGTCT630.8540056933712891No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAAGTGTCTGCCACATAGAA620.8404500474447607No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCATGAGAAAAGGTGGGCC620.8404500474447607No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTTGTGCCTGCCTTAAGTAA570.7726718178121188No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAACATATGGCCAATAGCGT570.7726718178121188No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTTGAGTAGACACAGCTTGA560.7591161718855903No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATGCGCAAAGATTTAAAAA550.745560525959062No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAAAAGCCAAAGGAGGATGG540.7320048800325335No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATCTGCGACATCCCTGGGA540.7320048800325335No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAAGTCTGCATGGAGCAGGA520.7048935881794768No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTTACGATACACGTCTGCAG510.6913379422529483No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAACAAACAATAAAACCTTT500.67778229632642No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCCCAGTCAAGCATATTGA500.67778229632642No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAGCCCTACTCAGGTTAATG500.67778229632642No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATGAAACTTGTTTTCCAAA490.6642266503998916No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAACAGGGATATTACCTACC490.6642266503998916No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTACAAACCAGGATTCTAGC480.6506710044733631No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTATGGTGCTCTTCCCTCCT460.6235597126203063No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTTGGTTAAGCATTTATGTG450.6100040666937779No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTATTCAGTTGCCTGAAGAG420.5693371289141927No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTAGCAGAACACTCTTTTCT400.5422258370611359No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTACAAAGCCTCAAATGTAA390.5286701911346076No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTAGGGTTTAGTGACTATTG390.5286701911346076No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTTGTGTTACTAATGACTGT360.4880032533550223No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTTTTCCCAAGGTCTCAGTG360.4880032533550223No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTAGTGCCTTCCCATTGCCT360.4880032533550223No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTACTGTCGCAAACAAGACT350.47444760742849396No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATGGTTGCCACCTTGTTAC340.46089196150196554No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTAGGAAATGCTCTTATTAT320.4337806696489087No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATTTCCCTGTCATACCCCC310.42022502372238035No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTACCTTATTTCCCCCCATT300.40666937779585194No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCTAGATTGACGCCTTTTG280.37955808594279516No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCGGCATCTTTTGGTTTGC270.36600244001626675No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTAGGTGCACTGTAATAATC250.33889114816321No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTAGATTCCATAGAGATATT230.31177985631015315No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCGAGTAGACACAGCTTGA180.24400162667751116No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCAGGAGCTGGAGGCAGAG170.23044598075098277No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATGGAGAGACCAAGTCTCTGCTACCGTAATACTCGCGT160.21689033482445436No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCGGTTAAGCATTTATGTG150.20333468889792597No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCGTGCCTGCCTTAAGTAA140.18977904297139758No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTGTGCCTGCCTTAAGTAAT130.1762233970448692No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCCAAAGCCTCAAATGTAA130.1762233970448692No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCGCAGAACACTCTTTTCT120.16266775111834078No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCTCCATGTAGAACCATGTCGTCAGTGTATACTCGCGT120.16266775111834078No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCTGGTGCTCTTCCCTCCT110.1491121051918124No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCTTCCCAAGGTCTCAGTG110.1491121051918124No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTACCTGATTTCTTTAGGCC100.13555645926528398No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCAGCCCACCCTGGATTAA100.13555645926528398No Hit
ACGCGAGTATACACTGACGACATGGTTCTACAACAGGGATATTACAGTGC100.13555645926528398No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTAGGTGCACTGTAATATCC100.13555645926528398No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCCCAGTCAAGGGAAGATT100.13555645926528398No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCGTGTTACTAATGACTGT100.13555645926528398No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTACCTTATTTCCCCCCCAT100.13555645926528398No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCCCAGAGGAGGAGTATGT100.13555645926528398No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCTTCAGTTGCCTGAAGAG100.13555645926528398No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCCTGTCGCAAACAAGACT90.12200081333875558No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTGTTCAGTTGCCTGAAGAG90.12200081333875558No Hit
ACGCGAGTATACACTGACGACATGGTTCTACATAATCAGTGTGATTCGGA90.12200081333875558No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCAACAGCAGATGGTCATA90.12200081333875558No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCCCAAGCAAGGCAAACAC90.12200081333875558No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCGGGTTTAGTGACTATTG80.10844516741222718No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCAGGACCCATTAGAACCA80.10844516741222718No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTGCCTTATTTCCCCCCATT80.10844516741222718No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCGGTGCACTGTAATAATC80.10844516741222718No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCACGATACACGTCTGCAG80.10844516741222718No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCCCTGATTTCTTTAGGCC80.10844516741222718No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCCAAGCAAGGCAAACACA80.10844516741222718No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTACCTTATTTCCCCCATTT80.10844516741222718No Hit
ACGCGAGTATTACGGTAGCAGAGACTTGGTCTCGGTGCACTGTAATATCC80.10844516741222718No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)