FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003090008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003090008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7416
Sequences flagged as poor quality0
Sequence length46-623
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATACGTCTACACTGACGACATGGTTCTACATAGGGCTCCCTGGACCCA1141.5372168284789645No Hit
TGATACGTCTACACTGACGACATGGTTCTACATGGAAGCTAGAAGAAACA1121.5102481121898599No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCGGAACTGCTGTCTGCAT1081.4563106796116505No Hit
TGATACGTCTACACTGACGACATGGTTCTACATGGGCTAATTGCGGGACT1051.4158576051779936No Hit
TGATACGTCTACACTGACGACATGGTTCTACATGCCTGTGGATCCCTAGC1031.3888888888888888No Hit
TGATACGTCTACACTGACGACATGGTTCTACAGTGTGTCACTCGTAATTA1031.3888888888888888No Hit
TGATACGTCTACACTGACGACATGGTTCTACATGGAGAGAGTGAAGAAAC1021.3754045307443366No Hit
TGATACGTCTACACTGACGACATGGTTCTACAAGCTCTGTCAAACATAAA1001.348435814455232No Hit
TGATACGTCTACACTGACGACATGGTTCTACAATCCCGTGGCCTAAAGAA991.3349514563106795No Hit
TGATACGTCTACACTGACGACATGGTTCTACAGGTGAGTCACAGGTTCAG981.3214670981661272No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTGTAGTGAAAGTAGGAGGA981.3214670981661272No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCCCTTTTGCCTGCAGGAG971.3079827400215749No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTGCCCAGGCCTTTCTCCAC941.267529665587918No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTGCCTTACTGAGGCTTGCT941.267529665587918No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCTAACTCCTGGTGTCTCT941.267529665587918No Hit
TGATACGTCTACACTGACGACATGGTTCTACAGGGAAAGGGCGTCATCAG931.2540453074433657No Hit
TGATACGTCTACACTGACGACATGGTTCTACACCTGCGCATGTACACTCA931.2540453074433657No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTTCCAGAGGAGGAGTATGT921.2405609492988134No Hit
TGATACGTCTACACTGACGACATGGTTCTACAAATCTTCCCCTTGACTTT921.2405609492988134No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCTCCCCACCAGACCATGA901.2135922330097086No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTGAATGTCTGTGGTTTGGT891.2001078748651564No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTGAGCTCTGTGCCCTATCT861.1596548004314995No Hit
TGATACGTCTACACTGACGACATGGTTCTACATTCATGCGCCTTTCCATT861.1596548004314995No Hit
TGATACGTCTACACTGACGACATGGTTCTACATCGCATCACCAATGCCTT861.1596548004314995No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCCCAGTCAAGCATATTGA841.132686084142395No Hit
TGATACGTCTACACTGACGACATGGTTCTACAACATGCTACTTACCAGGT841.132686084142395No Hit
TGATACGTCTACACTGACGACATGGTTCTACATTCTAGCAGTATCGACAA831.1192017259978424No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCGTGCATCAGTGGCTTTT801.0787486515641855No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTTAGGAGCTGGAGGCAGAG801.0787486515641855No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTTGTGCCTGCCTTAAGTAA791.0652642934196332No Hit
TGATACGTCTACACTGACGACATGGTTCTACATCCCACAGCATGACCTAC781.051779935275081No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCACTATGGCTTAGCGTCT751.0113268608414239No Hit
TGATACGTCTACACTGACGACATGGTTCTACATCATGAGGGGAGGCAGCT740.9978425026968716No Hit
TGATACGTCTACACTGACGACATGGTTCTACAAGTCTGCATGGAGCAGGA740.9978425026968716No Hit
TGATACGTCTACACTGACGACATGGTTCTACATTTCTGTCCTTTGCAAGC720.9708737864077669No Hit
TGATACGTCTACACTGACGACATGGTTCTACAAGTTGCTGTCCTGTCACT720.9708737864077669No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTTAGCCCACCCTGGATTAA710.9573894282632146No Hit
TGATACGTCTACACTGACGACATGGTTCTACATGGAGGCAAAAGAAAGGC700.9439050701186623No Hit
TGATACGTCTACACTGACGACATGGTTCTACAAGAAGGCGTACATTTGTC690.9304207119741101No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCTTTTGCCTCCATCCTCA690.9304207119741101No Hit
TGATACGTCTACACTGACGACATGGTTCTACACCATTTATGAACTCTCAA690.9304207119741101No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTTACGATACACGTCTGCAG680.9169363538295576No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTATGGTGCTCTTCCCTCCT680.9169363538295576No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTGGCTGACCTAAAGCCACC670.9034519956850053No Hit
TGATACGTCTACACTGACGACATGGTTCTACACCCGAATTTTGCTAATCC660.889967637540453No Hit
TGATACGTCTACACTGACGACATGGTTCTACAAGCCATGAAACGAACCTT660.889967637540453No Hit
TGATACGTCTACACTGACGACATGGTTCTACACGATGAACAATGACATCT660.889967637540453No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTGGCGTTTCTAATAATGCC640.8629989212513484No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTATTTCCTCTGCCGTGTTT640.8629989212513484No Hit
TGATACGTCTACACTGACGACATGGTTCTACAACAGCTTTTCCTCCATGA640.8629989212513484No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTAAGGACCCATTAGAACCA640.8629989212513484No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTGTGGTTGTCATCAAGAAA640.8629989212513484No Hit
TGATACGTCTACACTGACGACATGGTTCTACAACATATGGCCAATAGCGT610.8225458468176915No Hit
TGATACGTCTACACTGACGACATGGTTCTACATCATGAAAATGGTCAGAG600.8090614886731391No Hit
TGATACGTCTACACTGACGACATGGTTCTACATCTGCGACATCCCTGGGA600.8090614886731391No Hit
TGATACGTCTACACTGACGACATGGTTCTACATAATCAGTGTGATTCGTG600.8090614886731391No Hit
TGATACGTCTACACTGACGACATGGTTCTACAGGCCTAATGATTATTTTT590.7955771305285868No Hit
TGATACGTCTACACTGACGACATGGTTCTACAAGCAAGGGATTGTGATTG590.7955771305285868No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTTGAGTAGACACAGCTTGA590.7955771305285868No Hit
TGATACGTCTACACTGACGACATGGTTCTACAAGTGTCTGCCACATAGAA590.7955771305285868No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTTCCAAGCAAGGCAAACAC580.7820927723840345No Hit
TGATACGTCTACACTGACGACATGGTTCTACACCATGGACACTGGATTAA570.7686084142394822No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCATGAGAAAAGGTGGGCC560.7551240560949299No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTTGGTTAAGCATTTATGTG550.7416396979503775No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTAAACAGCAGATGGTCATA550.7416396979503775No Hit
TGATACGTCTACACTGACGACATGGTTCTACAGCCCTACTCAGGTTAATG540.7281553398058253No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTATTCAGTTGCCTGAAGAG530.7146709816612729No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTACAAAGCCTCAAATGTAA530.7146709816612729No Hit
TGATACGTCTACACTGACGACATGGTTCTACATGAAACTTGTTTTCCAAA500.674217907227616No Hit
TGATACGTCTACACTGACGACATGGTTCTACATAACTTGGGCTTTCTGAC490.6607335490830636No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTACCTTATTTCCCCCCATT480.6472491909385114No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTACAAACCAGGATTCTAGC470.633764832793959No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCTAGATTGACGCCTTTTG460.6202804746494067No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTTTTCCCAAGGTCTCAGTG450.6067961165048543No Hit
TGATACGTCTACACTGACGACATGGTTCTACAAAAGCCAAAGGAGGATGG450.6067961165048543No Hit
TGATACGTCTACACTGACGACATGGTTCTACAACAGGGATATTACAGTGC440.593311758360302No Hit
TGATACGTCTACACTGACGACATGGTTCTACATTTCCCTGTCATACCCCC410.5528586839266451No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTGGATCCTGGCTCCTTATC410.5528586839266451No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTAGGGTTTAGTGACTATTG410.5528586839266451No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCGGCATCTTTTGGTTTGC410.5528586839266451No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTTGTGTTACTAATGACTGT400.5393743257820928No Hit
TGATACGTCTACACTGACGACATGGTTCTACATGCGCAAAGATTTAAAAA400.5393743257820928No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTAGTGCCTTCCCATTGCCT390.5258899676375405No Hit
TGATACGTCTACACTGACGACATGGTTCTACAACAAACAATAAAACCTTT360.48543689320388345No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTAGCAGAACACTCTTTTCT340.4584681769147788No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTACTGTCGCAAACAAGACT330.4449838187702265No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTAGATTCCATAGAGATATT300.40453074433656955No Hit
TGATACGTCTACACTGACGACATGGTTCTACATGGTTGCCACCTTGTTAC270.3640776699029126No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTAGGTGCACTGTAATATCC270.3640776699029126No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCGCAGAACACTCTTTTCT210.28317152103559873No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCAGGAGCTGGAGGCAGAG190.2562028047464941No Hit
TGATACGTCTACACTGACGACATGGTTCTACAGACCAAGTCTCTGCTACCGTAAGACGTATCA180.24271844660194172No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTAGGAAATGCTCTTATTAT180.24271844660194172No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCGGTTAAGCATTTATGTG170.2292340884573894No Hit
TGATACGTCTACACTGACGACATGGTTCTACAGTTTGTTCACACAGACCA170.2292340884573894No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCGTGTTACTAATGACTGT160.2157497303128371No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTAGGTGCACTGTAATAATC160.2157497303128371No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTGTGTGAACAAACTGTAGA140.18878101402373249No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCGGTGCACTGTAATATCC140.18878101402373249No Hit
TGATACGTCTACACTGACGACATGGTTCTACAACAGGGATATTACCTACC140.18878101402373249No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCGAGTAGACACAGCTTGA140.18878101402373249No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTGTAGAACCATGTCGTCAGTGTAGACGTATCA140.18878101402373249No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCTGGTGCTCTTCCCTCCT120.16181229773462785No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCGTGCCTGCCTTAAGTAA120.16181229773462785No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCGGGTTTAGTGACTATTG120.16181229773462785No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCGTGTAGAACCATGTCGTCAGTGTAGACGTATCA110.1483279395900755No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCAGGACCCATTAGAACCA110.1483279395900755No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCTTCAGTTGCCTGAAGAG100.1348435814455232No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCCCAGAGGAGGAGTATGT100.1348435814455232No Hit
TGATACGTCTACACTGACGACATGGTTCTACATCATGAAATTCCAGTTGA100.1348435814455232No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCCTGTCGCAAACAAGACT100.1348435814455232No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCCAGAGGAGGAGTATGTG90.12135922330097086No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCCCTGATTTCTTTAGGCC90.12135922330097086No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCACGATACACGTCTGCAG90.12135922330097086No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCGATTCCATAGAGATATT90.12135922330097086No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTACCTGATTTCTTTAGGCC90.12135922330097086No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCGGAAATGCTCTTATTAT90.12135922330097086No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCCAAACCAGGATTCTAGC90.12135922330097086No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCAGTGTAGAACCATGTCGTCAGTGTAGACGTATCA80.10787486515641855No Hit
TGATACGTCTACACTGACGACATGGTTCTACATTTGAGGCTTTGTAGACC80.10787486515641855No Hit
TGATACGTCTACACTGACGACATGGTTCTACATGGAGAGACCAAGTCTCTGCTACCGTAAGACGTATCA80.10787486515641855No Hit
TGATACGTCTTACGGTAGCAGAGACTTGGTCTCTTTCCTCTGCCGTGTTT80.10787486515641855No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)