FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003090009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003090009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7888
Sequences flagged as poor quality0
Sequence length47-551
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGCAGTAACACTGACGACATGGTTCTACATAGGGCTCCCTGGACCCA1401.7748478701825559No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATGGGCTAATTGCGGGACT1271.6100405679513183No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTGCCCAGGCCTTTCTCCAC1141.445233265720081No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACACCATTTATGAACTCTCAA1111.4072008113590264No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAGGTGAGTCACAGGTTCAG1071.3564908722109534No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATGGAGAGAGTGAAGAAAC1041.3184584178498986No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCGGAACTGCTGTCTGCAT1041.3184584178498986No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATTCATGCGCCTTTCCATT1031.3057809330628805No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAAGCTCTGTCAAACATAAA1021.293103448275862No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTGAGCTCTGTGCCCTATCT1011.280425963488844No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAAATCTTCCCCTTGACTTT1001.2677484787018256No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAGGCCTAATGATTATTTTT1001.2677484787018256No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAGTGTGTCACTCGTAATTA991.2550709939148075No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCACTATGGCTTAGCGTCT981.2423935091277891No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATTCTAGCAGTATCGACAA971.2297160243407708No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACACCTGCGCATGTACACTCA971.2297160243407708No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCCCTTTTGCCTGCAGGAG971.2297160243407708No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAAGTCTGCATGGAGCAGGA971.2297160243407708No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAAGCAAGGGATTGTGATTG951.2043610547667343No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATGCCTGTGGATCCCTAGC931.1790060851926978No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTGGCTGACCTAAAGCCACC931.1790060851926978No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATCATGAGGGGAGGCAGCT921.1663286004056794No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATCGCATCACCAATGCCTT911.1536511156186613No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATGGAAGCTAGAAGAAACA901.140973630831643No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTATTTCCTCTGCCGTGTTT901.140973630831643No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAATCCCGTGGCCTAAAGAA891.1282961460446248No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAAGTGTCTGCCACATAGAA881.1156186612576064No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTTAGGAGCTGGAGGCAGAG871.1029411764705883No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATAACTTGGGCTTTCTGAC871.1029411764705883No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAGGGAAAGGGCGTCATCAG871.1029411764705883No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTGTAGTGAAAGTAGGAGGA851.0775862068965518No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACACCATGGACACTGGATTAA831.0522312373225153No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATCCCACAGCATGACCTAC801.0141987829614605No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACACGATGAACAATGACATCT801.0141987829614605No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAAGTTGCTGTCCTGTCACT791.0015212981744424No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTTCCAGAGGAGGAGTATGT791.0015212981744424No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATGGAGGCAAAAGAAAGGC791.0015212981744424No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAACATGCTACTTACCAGGT780.9888438133874239No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCTTTTGCCTCCATCCTCA780.9888438133874239No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTTGTGCCTGCCTTAAGTAA770.9761663286004058No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAAGCCATGAAACGAACCTT730.9254563894523328No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTGCCTTACTGAGGCTTGCT730.9254563894523328No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATGAAACTTGTTTTCCAAA730.9254563894523328No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCCCAGTCAAGCATATTGA720.9127789046653144No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTGGCGTTTCTAATAATGCC720.9127789046653144No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTGAATGTCTGTGGTTTGGT720.9127789046653144No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAACATATGGCCAATAGCGT710.9001014198782962No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTGTGGTTGTCATCAAGAAA700.8874239350912779No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCGTGCATCAGTGGCTTTT670.8493914807302232No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATGCGCAAAGATTTAAAAA660.8367139959432048No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTTAGCCCACCCTGGATTAA660.8367139959432048No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCTAACTCCTGGTGTCTCT640.8113590263691683No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTATTCAGTTGCCTGAAGAG640.8113590263691683No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCTCCCCACCAGACCATGA630.7986815415821502No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACACCCGAATTTTGCTAATCC630.7986815415821502No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAACAGGGATATTACCTACC620.7860040567951319No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTAAGGACCCATTAGAACCA620.7860040567951319No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTTCCAAGCAAGGCAAACAC610.7733265720081136No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACACGCAGCTGGGGACTTTTT610.7733265720081136No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAAAAGCCAAAGGAGGATGG600.7606490872210954No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTATGGTGCTCTTCCCTCCT590.7479716024340771No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATAATCAGTGTGATTCGTG580.7352941176470588No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTTACGATACACGTCTGCAG570.7226166328600405No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATTTCTGTCCTTTGCAAGC570.7226166328600405No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCATGAGAAAAGGTGGGCC570.7226166328600405No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTGGATCCTGGCTCCTTATC570.7226166328600405No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATCATGAAAATGGTCAGAG560.7099391480730223No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAAGAAGGCGTACATTTGTC550.697261663286004No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAACAGCTTTTCCTCCATGA550.697261663286004No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTTGAGTAGACACAGCTTGA540.6845841784989858No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAGCCCTACTCAGGTTAATG540.6845841784989858No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTTGGTTAAGCATTTATGTG520.6592292089249493No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTACAAAGCCTCAAATGTAA510.646551724137931No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATCTGCGACATCCCTGGGA510.646551724137931No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAACAAACAATAAAACCTTT500.6338742393509128No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTACTGTCGCAAACAAGACT470.595841784989858No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTAGTGCCTTCCCATTGCCT460.5831643002028397No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTACAAACCAGGATTCTAGC460.5831643002028397No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCGGCATCTTTTGGTTTGC450.5704868154158215No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTTTTCCCAAGGTCTCAGTG440.5578093306288032No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTACCTGATTCCCATTAACA410.5197768762677485No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTAGGGTTTAGTGACTATTG390.49442190669371194No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTAGGTGCACTGTAATAATC380.4817444219066937No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTAAACAGCAGATGGTCATA370.46906693711967545No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTTGTGTTACTAATGACTGT370.46906693711967545No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTACCTTATTTCCCCCCATT360.4563894523326572No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTAGCAGAACACTCTTTTCT310.39300202839756593No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATGGTTGCCACCTTGTTAC310.39300202839756593No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTAGATTCCATAGAGATATT270.3422920892494929No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTACCTTATTTCCCCCATTT230.29158215010141986No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAACAGGGATATTACAGTGC200.2535496957403651No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCCCAGAGGAGGAGTATGT190.24087221095334685No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTAGGTGCACTGTAATATCC170.21551724137931033No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTAGGAAATGCTCTTATTAT170.21551724137931033No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCGTGTTACTAATGACTGT160.2028397565922921No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCGGTTAAGCATTTATGTG150.19016227180527384No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCGGAAATGCTCTTATTAT140.17748478701825557No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCCAAACCAGGATTCTAGC140.17748478701825557No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTGTTCAGTTGCCTGAAGAG130.16480730223123732No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCTCCATGTAGAACCATGTCGTCAGTGTTACTGCTAGT130.16480730223123732No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACATGGAGAGACCAAGTCTCTGCTACCGTATACTGCTAGT130.16480730223123732No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCCCAAGCAAGGCAAACAC120.15212981744421905No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCGTGCCTGCCTTAAGTAA120.15212981744421905No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCAGCCCACCCTGGATTAA110.1394523326572008No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCTTCCCAAGGTCTCAGTG110.1394523326572008No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTGGTTAAGCATTTATGTGC110.1394523326572008No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCGATTCCATAGAGATATT110.1394523326572008No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCCAGAGGAGGAGTATGTG100.12677484787018256No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCCCTGATTTCTTTAGGCC100.12677484787018256No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCGGTGCACTGTAATAATC90.1140973630831643No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCGAGTAGACACAGCTTGA90.1140973630831643No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCCTGTCGCAAACAAGACT90.1140973630831643No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTGTGCCTGCCTTAAGTAAT90.1140973630831643No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCAGGAGCTGGAGGCAGAG90.1140973630831643No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCTGGTGCTCTTCCCTCCT90.1140973630831643No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCGGGTTTAGTGACTATTG80.10141987829614604No Hit
ACTAGCAGTAACACTGACGACATGGTTCTACAAATCTTCCCCTTGACTGG80.10141987829614604No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTCACGATACACGTCTGCAG80.10141987829614604No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTGTTTCCTCTGCCGTGTTT80.10141987829614604No Hit
ACTAGCAGTATACGGTAGCAGAGACTTGGTCTACCTGATTTCTTTAGGCC80.10141987829614604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)