FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003090012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003090012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7962
Sequences flagged as poor quality0
Sequence length46-606
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTATGCGACACTGACGACATGGTTCTACATAGGGCTCCCTGGACCCA1351.6955538809344386No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATGGGCTAATTGCGGGACT1351.6955538809344386No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCCCTTTTGCCTGCAGGAG1201.5071590052750565No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCGGAACTGCTGTCTGCAT1161.456920371765888No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATGGAGAGAGTGAAGAAAC1121.4066817382567194No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAATCCCGTGGCCTAAAGAA1101.3815624215021352No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTTAGGAGCTGGAGGCAGAG1091.3690027631248431No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTGCCTTACTGAGGCTTGCT1041.3062044712383825No Hit
TCTCTATGCGACACTGACGACATGGTTCTACACCATTTATGAACTCTCAA1031.29364481286109No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATCGCATCACCAATGCCTT1021.281085154483798No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAAGCTCTGTCAAACATAAA1021.281085154483798No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAACATGCTACTTACCAGGT1011.268525496106506No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATGCCTGTGGATCCCTAGC1001.2559658377292138No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAGGGAAAGGGCGTCATCAG971.2182868625973373No Hit
TCTCTATGCGACACTGACGACATGGTTCTACACCTGCGCATGTACACTCA971.2182868625973373No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATTCTAGCAGTATCGACAA941.180607887465461No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTGTAGTGAAAGTAGGAGGA941.180607887465461No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAGGTGAGTCACAGGTTCAG941.180607887465461No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATCATGAGGGGAGGCAGCT911.1429289123335846No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATGGAAGCTAGAAGAAACA911.1429289123335846No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAAATCTTCCCCTTGACTTT911.1429289123335846No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATTCATGCGCCTTTCCATT891.1178095955790002No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAGTGTGTCACTCGTAATTA881.1052499372017082No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAAGCAAGGGATTGTGATTG881.1052499372017082No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTGAGCTCTGTGCCCTATCT881.1052499372017082No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAAGAAGGCGTACATTTGTC881.1052499372017082No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCGTGCATCAGTGGCTTTT861.080130620447124No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTAAGGACCCATTAGAACCA841.0550113036925395No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAACAGCTTTTCCTCCATGA841.0550113036925395No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTGCCCAGGCCTTTCTCCAC821.0298919869379553No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCCCAGTCAAGCATATTGA821.0298919869379553No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCTCCCCACCAGACCATGA821.0298919869379553No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTTCCAGAGGAGGAGTATGT811.0173323285606632No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTGTGGTTGTCATCAAGAAA811.0173323285606632No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATTTCTGTCCTTTGCAAGC811.0173323285606632No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATCCCACAGCATGACCTAC811.0173323285606632No Hit
TCTCTATGCGACACTGACGACATGGTTCTACACGATGAACAATGACATCT811.0173323285606632No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTGGCTGACCTAAAGCCACC801.004772670183371No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTTAGCCCACCCTGGATTAA790.9922130118060788No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAAGTCTGCATGGAGCAGGA780.9796533534287867No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATAATCAGTGTGATTCGTG750.9419743782969104No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCTTTTGCCTCCATCCTCA740.9294147199196181No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTGAATGTCTGTGGTTTGGT740.9294147199196181No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATGGAGGCAAAAGAAAGGC730.916855061542326No Hit
TCTCTATGCGACACTGACGACATGGTTCTACACCCGAATTTTGCTAATCC730.916855061542326No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAACATATGGCCAATAGCGT730.916855061542326No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTACAAAGCCTCAAATGTAA730.916855061542326No Hit
TCTCTATGCGACACTGACGACATGGTTCTACACCATGGACACTGGATTAA730.916855061542326No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAGGCCTAATGATTATTTTT720.9042954031650339No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAAGCCATGAAACGAACCTT720.9042954031650339No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTGGATCCTGGCTCCTTATC710.8917357447877419No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAAGTTGCTGTCCTGTCACT700.8791760864104498No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATCATGAAAATGGTCAGAG700.8791760864104498No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCTAACTCCTGGTGTCTCT700.8791760864104498No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCATGAGAAAAGGTGGGCC700.8791760864104498No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCACTATGGCTTAGCGTCT700.8791760864104498No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATAACTTGGGCTTTCTGAC670.8414971112785732No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTTACGATACACGTCTGCAG670.8414971112785732No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAAGTGTCTGCCACATAGAA670.8414971112785732No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTGGCGTTTCTAATAATGCC640.8038181361466967No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAACAGGGATATTACCTACC640.8038181361466967No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATGAAACTTGTTTTCCAAA630.7912584777694046No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAGCCCTACTCAGGTTAATG610.7661391610148204No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTTGGTTAAGCATTTATGTG610.7661391610148204No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATCTGCGACATCCCTGGGA580.728460185882944No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTACAAACCAGGATTCTAGC570.7159005275056519No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTATTTCCTCTGCCGTGTTT570.7159005275056519No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTAAACAGCAGATGGTCATA560.7033408691283597No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTTGTGTTACTAATGACTGT560.7033408691283597No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTTCCAAGCAAGGCAAACAC550.6907812107510676No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTTGTGCCTGCCTTAAGTAA550.6907812107510676No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTAGTGCCTTCCCATTGCCT540.6782215523737753No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTATTCAGTTGCCTGAAGAG520.6531022356191912No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTTGAGTAGACACAGCTTGA510.640542577241899No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTATGGTGCTCTTCCCTCCT510.640542577241899No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTAGGGTTTAGTGACTATTG500.6279829188646069No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTACCTTATTTCCCCCCATT490.6154232604873147No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTAGCAGAACACTCTTTTCT480.6028636021100227No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTTTTCCCAAGGTCTCAGTG470.5903039437327305No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATGCGCAAAGATTTAAAAA450.5651846269781462No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAACAGGGATATTACAGTGC450.5651846269781462No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCTAGATTGACGCCTTTTG440.5526249686008541No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAACAAACAATAAAACCTTT430.540065310223562No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATTTCCCTGTCATACCCCC430.540065310223562No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTACTGTCGCAAACAAGACT390.48982667671439334No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTAGGTGCACTGTAATAATC360.45214770158251694No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAAAAGCCAAAGGAGGATGG310.3893494096960563No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTAGGAAATGCTCTTATTAT300.37678975131876413No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTAGATTCCATAGAGATATT280.35167043456417985No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCGGCATCTTTTGGTTTGC260.3265511178095956No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATGGTTGCCACCTTGTTAC230.28887214267771916No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTAGGTGCACTGTAATATCC210.26375282592313487No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTACCTTATTTCCCCCATTT200.25119316754584275No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCGGTTAAGCATTTATGTG160.20095453403667418No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCGGGTTTAGTGACTATTG130.1632755589047978No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCCCAGAGGAGGAGTATGT130.1632755589047978No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCGCAGAACACTCTTTTCT130.1632755589047978No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCGGTGCACTGTAATATCC110.13815624215021352No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCGTGTTACTAATGACTGT110.13815624215021352No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCGGAAATGCTCTTATTAT110.13815624215021352No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCTCCATGTAGAACCATGTCGTCAGTGTCGCATAGAGA110.13815624215021352No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAGACCAAGTCTCTGCTACCGTACGCATAGAGA110.13815624215021352No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCGATTCCATAGAGATATT110.13815624215021352No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATGGAGAGACCAAGTCTCTGCTACCGTACGCATAGAGA110.13815624215021352No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCCCAAGCAAGGCAAACAC100.12559658377292138No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCGTGCCTGCCTTAAGTAA100.12559658377292138No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCGTGCCTTCCCATTGCCT100.12559658377292138No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCTTCCCAAGGTCTCAGTG100.12559658377292138No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCAGGAGCTGGAGGCAGAG100.12559658377292138No Hit
TCTCTATGCGACACTGACGACATGGTTCTACAAATCTTCCCCTTGACTGG100.12559658377292138No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCTGGTGCTCTTCCCTCCT100.12559658377292138No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCTTCAGTTGCCTGAAGAG100.12559658377292138No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCGGTGCACTGTAATAATC90.11303692539562923No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCCCTTATTTCCCCCCATT90.11303692539562923No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCCAAACCAGGATTCTAGC80.10047726701833709No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCTTTCCTCTGCCGTGTTT80.10047726701833709No Hit
TCTCTATGCGACACTGACGACATGGTTCTACATCATGAAATTCCAGTTGA80.10047726701833709No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTCCAAAGCCTCAAATGTAA80.10047726701833709No Hit
TCTCTATGCGTACGGTAGCAGAGACTTGGTCTACCTGATTTCTTTAGGCC80.10047726701833709No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)