FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003090013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003090013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14845
Sequences flagged as poor quality0
Sequence length47-676
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGTGCGTACACTGACGACATGGTTCTACATAGGGCTCCCTGGACCCA2221.495453014482991No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGGGCTAATTGCGGGACT2001.3472549680026944No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAATCCCGTGGCCTAAAGAA1931.3001010441226002No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAGCTCTGTCAAACATAAA1901.2798922196025597No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAGGTGAGTCACAGGTTCAG1881.2664196699225327No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGGAGAGAGTGAAGAAAC1841.239474570562479No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCTAACTCCTGGTGTCTCT1791.2057931963624116No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAGTGTGTCACTCGTAATTA1771.1923206466823846No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAATCTTCCCCTTGACTTT1761.1855843718423713No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATCGCATCACCAATGCCTT1751.1788480970023578No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAACATGCTACTTACCAGGT1731.1653755473223308No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATTCATGCGCCTTTCCATT1721.1586392724823174No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACACCATTTATGAACTCTCAA1671.12495789828225No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACACGATGAACAATGACATCT1661.1182216234422366No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATCTGCGACATCCCTGGGA1591.0710676995621422No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGGAACTGCTGTCTGCAT1581.0643314247221287No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACACCTGCGCATGTACACTCA1561.0508588750421017No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGCCCAGGCCTTTCTCCAC1561.0508588750421017No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACACCATGGACACTGGATTAA1541.0373863253620748No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGTGCATCAGTGGCTTTT1541.0373863253620748No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATAATCAGTGTGATTCGTG1531.0306500505220613No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGGAAGCTAGAAGAAACA1511.0171775008420343No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCTTTTGCCTCCATCCTCA1511.0171775008420343No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGCCTGTGGATCCCTAGC1501.0104412260020208No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATTCTAGCAGTATCGACAA1480.9969686763219939No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAGAAGGCGTACATTTGTC1470.9902324014819804No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAGGCCTAATGATTATTTTT1470.9902324014819804No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAGTCTGCATGGAGCAGGA1440.9700235769619401No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGCGCAAAGATTTAAAAA1430.9632873021219266No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGGAGGCAAAAGAAAGGC1420.9565510272819131No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAGGGAAAGGGCGTCATCAG1400.9430784776018861No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGCCTTACTGAGGCTTGCT1400.9430784776018861No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCCCTTTTGCCTGCAGGAG1380.9296059279218591No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAGCCCTACTCAGGTTAATG1370.9228696530818457No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATCCCACAGCATGACCTAC1330.8959245537217919No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAACATATGGCCAATAGCGT1320.8891882788817784No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGGCTGACCTAAAGCCACC1310.8824520040417649No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATTTCTGTCCTTTGCAAGC1300.8757157292017514No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGAAACTTGTTTTCCAAA1300.8757157292017514No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGTAGTGAAAGTAGGAGGA1300.8757157292017514No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCACTATGGCTTAGCGTCT1290.868979454361738No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGAGCTCTGTGCCCTATCT1270.855506904681711No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAGTGTCTGCCACATAGAA1210.8150892556416302No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACACCCGAATTTTGCTAATCC1180.7948804311215897No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTAGGAGCTGGAGGCAGAG1180.7948804311215897No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAGCAAGGGATTGTGATTG1170.7881441562815762No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGGCGTTTCTAATAATGCC1170.7881441562815762No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATCATGAGGGGAGGCAGCT1160.7814078814415627No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATAACTTGGGCTTTCTGAC1160.7814078814415627No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGAATGTCTGTGGTTTGGT1160.7814078814415627No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAACAGCTTTTCCTCCATGA1150.7746716066015493No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATCATGAAAATGGTCAGAG1140.7679353317615358No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATTTCCCTGTCATACCCCC1110.7477265072414955No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGTGGTTGTCATCAAGAAA1100.740990232401482No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTAGCCCACCCTGGATTAA1090.7342539575614685No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAGCCATGAAACGAACCTT1090.7342539575614685No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAGTTGCTGTCCTGTCACT1070.7207814078814416No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCCCAGTCAAGCATATTGA1060.7140451330414281No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCTCCCCACCAGACCATGA1060.7140451330414281No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCATGAGAAAAGGTGGGCC1050.7073088582014146No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTGTGCCTGCCTTAAGTAA1020.6871000336813742No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTGGTTAAGCATTTATGTG1000.6736274840013472No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTATTTCCTCTGCCGTGTTT990.6668912091613337No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTACGATACACGTCTGCAG980.6601549343213203No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTAGATTCCATAGAGATATT970.6534186594813068No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAACAAACAATAAAACCTTT970.6534186594813068No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTATGGTGCTCTTCCCTCCT970.6534186594813068No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGGATCCTGGCTCCTTATC960.6466823846412934No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCTAGATTGACGCCTTTTG940.6332098349612664No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTCCAGAGGAGGAGTATGT860.5793196362411587No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTAAGGACCCATTAGAACCA860.5793196362411587No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAAAGCCAAAGGAGGATGG830.5591108117211183No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTACCTTATTTCCCCCCATT820.5523745368811048No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGGAGAGACCAAGTCTCTGCTACCGTAACGCACTCGT790.5321657123610644No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTATTCAGTTGCCTGAAGAG780.5254294375210509No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTACAAAGCCTCAAATGTAA780.5254294375210509No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTTTCCCAAGGTCTCAGTG770.5186931626810374No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGGCATCTTTTGGTTTGC740.49848433816099696No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTGAGTAGACACAGCTTGA740.49848433816099696No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTCCAAGCAAGGCAAACAC730.49174806332098353No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTAGGGTTTAGTGACTATTG710.47827551364095655No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTAGCAGAACACTCTTTTCT700.47153923880094306No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAACAGGGATATTACAGTGC700.47153923880094306No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTAAACAGCAGATGGTCATA690.46480296396092957No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTTGTGTTACTAATGACTGT660.4445941394408892No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTACAAACCAGGATTCTAGC640.43112158976086223No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCTCCATGTAGAACCATGTCGTCAGTGTACGCACTCGT570.3839676658807679No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTAGTGCCTTCCCATTGCCT560.3772313910407545No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATGGTTGCCACCTTGTTAC550.370495116200741No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTACTGTCGCAAACAAGACT540.36375884136072756No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAATCTTCCCCTTGACTGG540.36375884136072756No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTAGGAAATGCTCTTATTAT500.3368137420006736No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCCCAGAGGAGGAGTATGT500.3368137420006736No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGTAGAACCATGTCGTCAGTGTACGCACTCGT460.30986864264061975No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGGTTAAGCATTTATGTG420.28292354328056585No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAGACCAAGTCTCTGCTACCGTAACGCACTCGT410.2761872684405524No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCAGGAGCTGGAGGCAGAG370.24924216908049848No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAACAGGGATATTACCTACC370.24924216908049848No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGTGCCTGCCTTAAGTAA350.23576961940047153No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTAGGTGCACTGTAATATCC350.23576961940047153No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCCCAGTCAAGGGAAGATT350.23576961940047153No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCCAAAGCCTCAAATGTAA340.2290333445604581No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGTGTTACTAATGACTGT330.2222970697204446No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGGTGCACTGTAATATCC330.2222970697204446No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGATTCCATAGAGATATT330.2222970697204446No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAGTCTGCATGGAGAGGAA330.2222970697204446No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCTGGTGCTCTTCCCTCCT330.2222970697204446No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCCCAAGCAAGGCAAACAC310.20882452004041765No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCCTGTCGCAAACAAGACT310.20882452004041765No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCCCTTATTTCCCCCCATT300.20208824520040417No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGGGTTTAGTGACTATTG290.19535197036039068No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGGAAATGCTCTTATTAT280.18861569552037724No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCCAAACCAGGATTCTAGC260.1751431458403503No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCCCTGATTTCTTTAGGCC250.1684068710003368No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGCAGAACACTCTTTTCT250.1684068710003368No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACACCATGTATGAAAGCCACT230.15493432132030988No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCTTCAGTTGCCTGAAGAG230.15493432132030988No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCACGATACACGTCTGCAG230.15493432132030988No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGTGGTTGTAGAACCATGTCGTCAGTGTACGCACTCGT220.1481980464802964No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGAGTAGACACAGCTTGA210.14146177164028292No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCAGCCCACCCTGGATTAA200.13472549680026946No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTACCTGATTTCTTTAGGCC200.13472549680026946No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCAACAGCAGATGGTCATA200.13472549680026946No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCTTTCCTCTGCCGTGTTT190.12798922196025597No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGTGCACTTTTCTTTGAG190.12798922196025597No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTACAAAGCCTCAAATGTAG180.12125294712024251No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCCCAGTCAAGGGGAAGAT170.11451667228022905No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCGGCATCTTTGCGCATGT170.11451667228022905No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAAAAAGCCAAAGGAGGATG160.10778039744021556No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTCAGGACCCATTAGAACCA160.10778039744021556No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACATACACTCTGAATATCTCT150.10104412260020208No Hit
ACGAGTGCGTACACTGACGACATGGTTCTACAACCACAGACCAAGTCTCTGCTACCGTAACGCACTCGT150.10104412260020208No Hit
ACGAGTGCGTTACGGTAGCAGAGACTTGGTCTGGCGTTTCTATGTGGCAG150.10104412260020208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)