FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003127398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003127398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4574265
Sequences flagged as poor quality0
Sequence length35-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC452070.9882899219874668TruSeq Adapter, Index 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG183350.40082942286902923No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77740.1699508008390419No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC77530.16949171069013275TruSeq Adapter, Index 1 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57340.1253534720878655No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)